A semi‐automated method to assess intraepidermal nerve fibre density in human skin biopsies

Evaluation of intraepidermal nerve fibres (IENFs) in skin biopsies is used in the diagnosis of small‐fibre neuropathies. The number of IENFs is assessed manually under a microscope, with an inter‐rater variability of ~25%. Unless the images are digitized, there is no documentation. Our aim was to develop a method for standardized semi‐automated quantification (SAQ) and documentation of IENF density.

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