Comparative Serological Assays for the Study of H5 and H7 Avian Influenza Viruses

The nature of influenza virus to randomly mutate and evolve into new types is an important challenge in the control of influenza infection. It is necessary to monitor virus evolution for a better understanding of the pandemic risk posed by certain variants as evidenced by the highly pathogenic avian influenza (HPAI) viruses. This has been clearly recognized in Egypt following the notification of the first HPAI H5N1 outbreak. The continuous circulation of the virus and the mass vaccination programme undertaken in poultry have resulted in a progressive genetic evolution and a significant antigenic drift near the major antigenic sites. In order to establish if vaccination is sufficient to provide significant intra- and interclade cross-protection, lentiviral pseudotypes derived from H5N1 HPAI viruses (A/Vietnam/1194/04, A/chicken/Egypt-1709-01/2007) and an antigenic drift variant (A/chicken/Egypt-1709-06-2008) were constructed and used in pseudotype-based neutralization assays (pp-NT). pp-NT data obtained was confirmed and correlated with HI and MN assays. A panel of pseudotypes belonging to influenza Groups 1 and 2, with a combination of reporter systems, was also employed for testing avian sera in order to support further application of pp-NT as an alternative valid assay that can improve avian vaccination efficacy testing, vaccine virus selection, and the reliability of reference sera.

[1]  E. Böttcher-Friebertshäuser,et al.  The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes , 2013, Journal of molecular and genetic medicine : an international journal of biomedical research.

[2]  E. Böttcher-Friebertshäuser,et al.  The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses , 2013, Journal of molecular and genetic medicine : an international journal of biomedical research.

[3]  C. Macken,et al.  Egyptian H5N1 Influenza Viruses—Cause for Concern? , 2012, PLoS pathogens.

[4]  N. Toft,et al.  Serological diagnosis of avian influenza in poultry: is the haemagglutination inhibition test really the ‘gold standard’? , 2012, Influenza and other respiratory viruses.

[5]  J. Peiris,et al.  Evaluation of serological tests for H5N1 avian influenza on field samples from domestic poultry populations in Vietnam: consequences for surveillance. , 2012, Veterinary microbiology.

[6]  Adolfo García-Sastre,et al.  Hemagglutinin stalk antibodies elicited by the 2009 pandemic influenza virus as a mechanism for the extinction of seasonal H1N1 viruses , 2012, Proceedings of the National Academy of Sciences.

[7]  R. Donis,et al.  Continued evolution of highly pathogenic avian influenza A (H5N1): updated nomenclature , 2011, Influenza and other respiratory viruses.

[8]  J. Weir,et al.  Determination of H5N1 vaccine potency using reference antisera from heterologous strains of influenza , 2011, Influenza and other respiratory viruses.

[9]  M. Beer,et al.  Multiple dose vaccination with heterologous H5N2 vaccine: immune response and protection against variant clade 2.2.1 highly pathogenic avian influenza H5N1 in broiler breeder chickens. , 2011, Vaccine.

[10]  M. Beer,et al.  Highly pathogenic avian influenza virus H5N1 from Egypt escapes vaccine-induced immunity but confers clinical protection against a heterologous clade 2.2.1 Egyptian isolate. , 2011, Vaccine.

[11]  M. Aly,et al.  Antigenic Drift in H5N1 Avian Influenza Virus in Poultry Is Driven by Mutations in Major Antigenic Sites of the Hemagglutinin Molecule Analogous to Those for Human Influenza Virus , 2011, Journal of Virology.

[12]  Dorothy Scott,et al.  Cross-Neutralizing Antibodies to Pandemic 2009 H1N1 and Recent Seasonal H1N1 Influenza A Strains Influenced by a Mutation in Hemagglutinin Subunit 2 , 2011, PLoS pathogens.

[13]  V. Deubel,et al.  Heterosubtypic Antibody Response Elicited with Seasonal Influenza Vaccine Correlates Partial Protection against Highly Pathogenic H5N1 Virus , 2011, PloS one.

[14]  G. Van Domselaar,et al.  Universal antibodies against the highly conserved influenza fusion peptide cross-neutralize several subtypes of influenza A virus. , 2010, Biochemical and biophysical research communications.

[15]  C. Davis,et al.  Antigenic and Genetic Diversity of Highly Pathogenic Avian Influenza A (H5N1) Viruses Isolated in Egypt , 2010, Avian diseases.

[16]  I. Monne,et al.  Evaluation of the protection induced by avian influenza vaccines containing a 1994 Mexican H5N2 LPAI seed strain against a 2008 Egyptian H5N1 HPAI virus belonging to clade 2.2.1 by means of serological and in vivo tests , 2010, Avian pathology : journal of the W.V.P.A.

[17]  R. Webster,et al.  Puzzling inefficiency of H5N1 influenza vaccines in Egyptian poultry , 2010, Proceedings of the National Academy of Sciences.

[18]  K. Subbarao,et al.  Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccine. , 2010, The Journal of clinical investigation.

[19]  Tara Anderson,et al.  FAO‐OIE‐WHO Joint Technical Consultation on Avian Influenza at the Human‐Animal Interface , 2010, Influenza and other respiratory viruses.

[20]  John Steel,et al.  Influenza Virus Vaccine Based on the Conserved Hemagglutinin Stalk Domain , 2010, mBio.

[21]  R. Hai,et al.  Broadly Protective Monoclonal Antibodies against H3 Influenza Viruses following Sequential Immunization with Different Hemagglutinins , 2010, PLoS pathogens.

[22]  L. Rudenko,et al.  Phenotypic characteristics of novel swine‐origin influenza A/California/07/2009 (H1N1) virus , 2009, Influenza and other respiratory viruses.

[23]  S. Cleaveland,et al.  A robust lentiviral pseudotype neutralisation assay for in-field serosurveillance of rabies and lyssaviruses in Africa , 2009, Vaccine.

[24]  K. Subbarao,et al.  Attacking the flu: Neutralizing antibodies may lead to 'universal' vaccine , 2009, Nature Medicine.

[25]  R. Rappuoli,et al.  Pseudoparticle neutralization is a reliable assay to measure immunity and cross-reactivity to H5N1 influenza viruses. , 2009, Vaccine.

[26]  N. Temperton,et al.  The Use of Retroviral Pseudotypes for the Measurement of Antibody Responses to SARS Coronavirus , 2009, Molecular Biology of the SARS-Coronavirus.

[27]  Gavin J. D. Smith,et al.  Continuing progress towards a unified nomenclature for the highly pathogenic H5N1 avian influenza viruses: Divergence of clade 2·2 viruses , 2009 .

[28]  M. Aly,et al.  Highly Pathogenic Avian Influenza Virus Subtype H5N1 in Africa: A Comprehensive Phylogenetic Analysis and Molecular Characterization of Isolates , 2009, PloS one.

[29]  I. Monne,et al.  Introduction into Nigeria of a Distinct Genotype of Avian Influenza Virus (H5N1) , 2009, Emerging infectious diseases.

[30]  C. Russell,et al.  Continuing progress towards a unified nomenclature for the highly pathogenic H5N1 avian influenza viruses: divergence of clade 2·2 viruses , 2009, Influenza and other respiratory viruses.

[31]  Boguslaw Stec,et al.  Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses , 2009, Nature Structural &Molecular Biology.

[32]  I. Capua,et al.  Avian influenza vaccines and vaccination in birds. , 2008, Vaccine.

[33]  R. Weiss,et al.  Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison , 2008, The Journal of general virology.

[34]  I. Capua,et al.  Control and prevention of avian influenza in an evolving scenario. , 2007, Vaccine.

[35]  I. Capua,et al.  Animal and Human Health Implications of Avian Influenza Infections , 2007, Bioscience reports.

[36]  R. Weiss,et al.  A sensitive retroviral pseudotype assay for influenza H5N1‐neutralizing antibodies , 2007, Influenza and other respiratory viruses.

[37]  G. Cattoli,et al.  Confirmation of H5N1 avian influenza in Africa , 2006, Veterinary Record.

[38]  N. Sargison,et al.  Sheep scab control in UK flocks , 2006, Veterinary Record.

[39]  D. Suarez,et al.  Development and Application of Reference Antisera against 15 Hemagglutinin Subtypes of Influenza Virus by DNA Vaccination of Chickens , 2006, Clinical and Vaccine Immunology.

[40]  Charles J. Russell,et al.  The Genesis of a Pandemic Influenza Virus , 2005, Cell.

[41]  A. Panlilio,et al.  Updated U.S. Public Health Service guidelines for the management of occupational exposures to HIV and recommendations for postexposure prophylaxis. , 2005, MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports.

[42]  R. Weiss,et al.  Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes , 2005, Emerging infectious diseases.

[43]  D. Suarez,et al.  Effect of Vaccine Use in the Evolution of Mexican Lineage H5N2 Avian Influenza Virus , 2004, Journal of Virology.

[44]  Malbea A Lapete,et al.  Morbidity and Mortality Weekly Report Prevention and Control of Influenza Recommendations of the Advisory Committee on Immunization Practices (acip) Centers for Disease Control and Prevention Epidemiology Program Office Early Release 1 Prevention and Control of Influenza Recommendations of the Advis , 2022 .

[45]  M. Thobaben,et al.  Prevention and Control of Influenza , 2003, MMWR. Morbidity and mortality weekly report.

[46]  Y. Takeuchi,et al.  Restriction of lentivirus in monkeys , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[47]  Kathryn L. Parsley,et al.  High-level transduction and gene expression in hematopoietic repopulating cells using a human imunodeficiency virus type 1-based lentiviral vector containing an internal spleen focus forming virus promoter , 2002 .

[48]  M. Zambon,et al.  Safety and antigenicity of non-adjuvanted and MF59-adjuvanted influenza A/Duck/Singapore/97 (H5N3) vaccine: a randomised trial of two potential vaccines against H5N1 influenza , 2001, The Lancet.

[49]  Yoshihiro Kawaoka,et al.  Pandemic Threat Posed by Avian Influenza A Viruses , 2001, Clinical Microbiology Reviews.

[50]  C. Bridges,et al.  Antibody response in individuals infected with avian influenza A (H5N1) viruses and detection of anti-H5 antibody among household and social contacts. , 1999, The Journal of infectious diseases.

[51]  Keiji Fukuda,et al.  Detection of Antibody to Avian Influenza A (H5N1) Virus in Human Serum by Using a Combination of Serologic Assays , 1999, Journal of Clinical Microbiology.

[52]  Luigi Naldini,et al.  Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo , 1997, Nature Biotechnology.

[53]  F. Gage,et al.  In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector , 1996, Science.

[54]  Yamamura Ken-ichi,et al.  Efficient selection for high-expression transfectants with a novel eukaryotic vector , 1991 .

[55]  H. Niwa,et al.  Efficient selection for high-expression transfectants with a novel eukaryotic vector. , 1991, Gene.

[56]  R. Webster,et al.  Do commercial avian influenza H5 vaccines induce cross-reactive antibodies against contemporary H5N1 viruses in Egypt? , 2013, Poultry science.

[57]  S. White,et al.  RNAi-mediated chromatin silencing in fission yeast. , 2008, Current topics in microbiology and immunology.

[58]  M. Osterholm,et al.  Preparing for the next pandemic. , 2005, The New England journal of medicine.

[59]  J. Banks,et al.  Additional Glycosylation at the Receptor Binding Site of the Hemagglutinin (HA) for H5 and H7 Viruses May Be an Adaptation to Poultry Hosts, but Does It Influence Pathogenicity? , 2003, Avian diseases.

[60]  I. Capua,et al.  Changes in the haemagglutinin and the neuraminidase genes prior to the emergence of highly pathogenic H7N1 avian influenza viruses in Italy , 2001, Archives of Virology.

[61]  R. Webster.  Influenza virus: transmission between species and relevance to emergence of the next human pandemic. , 1997, Archives of virology. Supplementum.

[62]  R. Harpaz,et al.  Prevention of plague: recommendations of the Advisory Committee on Immunization Practices (ACIP). , 1996, MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports.

[63]  G. Ada,et al.  The immune response to influenza infection. , 1986, Current topics in microbiology and immunology.