Analysis of saffron volatile fraction by TD–GC–MS and e-nose

Saffron spice is valuable for its colour, taste and aroma. All these properties depend on the different postharvesting treatments that Crocus sativus L. stigmas received to convert it into a spice. No previous scientific data relate the volatile content with its postharvesting process or origin. In this paper, two different analytical methods are used, CG-MS to determine volatile markers and an electronic nose based on metal oxides to determine the volatile profile of the samples from different geographic origins. It is demonstrated by means of CG-MS that 3,5,5-trimethyl-2-cyclohexenone, 2,6,6-trimethylcyclohexane-1,4-dione and acetic acid are capable to differentiate saffron from its origin. The content of acetic acid is very high in samples from Iran or Morocco, while low or undetectable content was observed in Greek and Spanish samples. In turn, using an e-nose (specifically optimised for this particular application), samples from different countries could be discriminated using Principal Component Analysis with a 90% of confidence.

[1]  Giorgio Sberveglieri,et al.  An electronic nose for the recognition of the vineyard of a red wine , 1996 .

[2]  Gian Luigi Rana,et al.  Volatile organic compounds from saffron , 2004 .

[3]  Wolfgang Rödel,et al.  Analysis of the volatile components of saffron , 1991 .

[4]  H. Troy Nagle,et al.  Handbook of Machine Olfaction: Electronic Nose Technology , 2003 .

[5]  M. Negbi.  Saffron: Crocus sativus L. , 2003 .

[6]  M. Sánchez-Fernández,et al.  Note. Safranal Content in Spanish Saffron , 2001 .

[7]  D. E. Heinz,et al.  Monoterpene aldehydes and isophorone-related compounds of saffron , 1971 .

[8]  P. Variyar,et al.  Chemical investigation of gamma-irradiated saffron (Crocus sativus L.). , 2001, Journal of agricultural and food chemistry.

[9]  M. Désage,et al.  Identification and Isotopic Analysis of Safranal from Supercritical Fluid Extraction and Alcoholic Extracts of Saffron , 1996 .

[10]  Felix Escher,et al.  Use of preconcentration techniques applied to a MS-based Electronic Nose , 2000 .

[11]  Moschos G. Polissiou,et al.  Isolation and Identification of the Aroma Components from Saffron (Crocus sativus) , 1997 .

[12]  A. Zalacain,et al.  Influence of different drying and aging conditions on saffron constituents. , 2005, Journal of agricultural and food chemistry.

[13]  Eduard Llobet,et al.  Selective methane detection under varying moisture conditions using static and dynamic sensor signals , 1999 .

[14]  H. T. Nagle,et al.  Handbook of Machine Olfaction , 2002 .

[15]  G. Alonso,et al.  Method to determine the authenticity of aroma of saffron (Crocus sativus L.). , 1998, Journal of food protection.

[16]  Giuseppe Ferri,et al.  An electronic nose for food analysis , 1997 .

[17]  L. Craker,et al.  Medicinal and Aromatic Plants , 2006 .

[18]  J. Gardner,et al.  Electronic noses and their application in the food industry , 1997 .

[19]  P. Tarantilis,et al.  Qualitative determination of volatile compounds and quantitative evaluation of safranal and 4-hydroxy-2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde (HTCC) in Greek saffron. , 2004, Journal of agricultural and food chemistry.