Timing of Plk1 and MPF activation during porcine oocyte maturation

A Polo‐like kinase 1 (Plk1) appears involved in an autocatalytic loop between CDC25C phosphatase and M phase promoting factor (MPF) in Xenopus oocytes and leads to activation of MPF that is required for germinal vesicle breakdown (GVBD). Although similar evidence for such a role of Plk1 in MPF activation during maturation of mammalian oocytes is absent, changes in Plk1 enzyme activity correlate with MPF activation, Plk1 co‐localizes with MPF, and microinjection of antibodies neutralizing Plk1 delays GVBD. In this study, we exploited the prolonged time required for maturation of porcine oocytes to define precisely the timing of Plk1 and MPF activation during maturation. GVBD typically occurs between 24 and 26 hr of culture in vitro and meiotic maturation is completed after 40–44‐hr culture. We find that Plk1 is activated before MPF, which is consistent with its role in activating MPF in mammalian oocytes. Mol. Reprod. Dev. 69: 11–16, 2004. © 2004 Wiley‐Liss, Inc.

[1]  IgM μ-Chain.  Antibodies , 2004, Nature Biotechnology.

[2]  T. Kishimoto.  Cell-cycle control during meiotic maturation. , 2003, Current opinion in cell biology.

[3]  H. Yoshida,et al.  Distinct regulators for Plk1 activation in starfish meiotic and early embryonic cycles , 2003, The EMBO journal.

[4]  F. Richard,et al.  Effect of cycloheximide, 6-DMAP, roscovitine and butyrolactone I on resumption of meiosis in porcine oocytes. , 2003, Theriogenology.

[5]  H. Niemann,et al.  Cell cycle dependent expression of Plk1 in synchronized porcine fetal fibroblasts , 2003, Molecular reproduction and development.

[6]  Zeng-Ming Yang,et al.  Characterization of polo‐like kinase‐1 in rat oocytes and early embryos implies its functional roles in the regulation of meiotic maturation, fertilization, and cleavage , 2003, Molecular reproduction and development.

[7]  D. Glover,et al.  Polar expeditions — provisioning the centrosome for mitosis , 2003, Nature Cell Biology.

[8]  R. Schultz,et al.  Chromosome condensation in pig oocytes: Lack of a requirement for either cdc2 kinase or MAP kinase activity , 2002, Molecular reproduction and development.

[9]  F. Uhlmann,et al.  Chromosome segregation: playing polo in prophase. , 2002, Developmental cell.

[10]  C. Jessus,et al.  From progesterone to active Cdc2 in Xenopus oocytes: a puzzling signalling pathway , 2001, Biology of the cell.

[11]  F. Taieb,et al.  The polo-like kinase Plx1 is required for activation of the phosphatase Cdc25C and cyclin B-Cdc2 in Xenopus oocytes. , 2001, Molecular biology of the cell.

[12]  M. Dorée,et al.  The interplay between cyclin-B-Cdc2 kinase (MPF) and MAP kinase during maturation of oocytes. , 2001, Journal of cell science.

[13]  R. Schultz,et al.  Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference. , 2000, Development.

[14]  E. Nigg,et al.  Characterization of polo-like kinase 1 during meiotic maturation of the mouse oocyte. , 2000, Developmental biology.

[15]  D. Longo,et al.  The polo‐like kinase PLK‐1 is required for nuclear envelope breakdown and the completion of meiosis in Caenorhabditis elegans , 2000, Genesis.

[16]  J. Maller,et al.  Mitotic Effects of a Constitutively Active Mutant of the Xenopus Polo-Like Kinase Plx1 , 1999, Molecular and Cellular Biology.

[17]  C. Jessus,et al.  Phosphatase 2A and polo kinase, two antagonistic regulators of cdc25 activation and MPF auto-amplification. , 1999, Journal of cell science.

[18]  D. Glover,et al.  Polo-like kinases: a team that plays throughout mitosis. , 1998, Genes & development.

[19]  E. Nigg.  Polo-like kinases: positive regulators of cell division from start to finish. , 1998, Current opinion in cell biology.

[20]  C. Jessus,et al.  MPF amplification in Xenopus oocyte extracts depends on a two-step activation of cdc25 phosphatase. , 1998, Experimental cell research.

[21]  J. Maller,et al.  Activated Polo-Like Kinase Plx1 Is Required at Multiple Points during Mitosis in Xenopus laevis , 1998, Molecular and Cellular Biology.

[22]  P. Kalab,et al.  Interplay between CDC2 kinase and MAP kinase pathway during maturation of mammalian oocytes. , 1998, Theriogenology.

[23]  A. Stutz,et al.  Acquisition of meiotic competence in growing mouse oocytes is controlled at both translational and posttranslational levels. , 1997, Developmental biology.

[24]  A. Kumagai,et al.  Purification and Molecular Cloning of Plx1, a Cdc25-Regulatory Kinase from Xenopus Egg Extracts , 1996, Science.

[25]  R. Schultz,et al.  Regulation of the acquisition of meiotic competence in the mouse: changes in the subcellular localization of cdc2, cyclin B1, cdc25C and wee1, and in the concentration of these proteins and their transcripts. , 1996, Journal of cell science.

[26]  K. Jones,et al.  Turning it on and off: M-phase promoting factor during meiotic maturation and fertilization. , 2004, Molecular human reproduction.