Quantum dots for biomedical applications.

BACKGROUND Nanotechnology is an emerging field that could have a significant impact on cancer diagnosis, treatment and analysis. Quantum dots represent one of the most interesting nanotechnology-based platforms, and their unique properties make them a potentially versatile tool for molecular diagnostics. OBJECTIVES Here, the most promising uses of quantum dots for translational research are reviewed, ranging from multiplexed immunofluorescence, targeted drug delivery coupled with disease visualization, tumor and sentinel organs localization and long-term cell tracing. Finally, potential future directions for quantum dots as both a diagnostic and therapeutic tool in biomedical research will be explored. CONCLUSIONS The use of quantum dots in biomedical applications is accelerating owing to their unique physical attributes, imaging capabilities, and potential for therapeutic delivery.

[1]  S. Pathak,et al.  Hydroxylated quantum dots as luminescent probes for in situ hybridization. , 2001, Journal of the American Chemical Society.

[2]  Byron Ballou,et al.  Noninvasive imaging of quantum dots in mice. , 2004, Bioconjugate chemistry.

[3]  Shan Jiang,et al.  Quantum-dot based nanoparticles for targeted silencing of HER2/neu gene via RNA interference. , 2007, Biomaterials.

[4]  Nigel J Walker,et al.  Migration of intradermally injected quantum dots to sentinel organs in mice. , 2007, Toxicological sciences : an official journal of the Society of Toxicology.

[5]  S. Pittaluga,et al.  Multispectral imaging of clinically relevant cellular targets in tonsil and lymphoid tissue using semiconductor quantum dots , 2006, Modern Pathology.

[6]  Philippe Rostaing,et al.  Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking , 2003, Science.

[7]  Shuming Nie,et al.  Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry , 2007, Nature Protocols.

[8]  Marina Sedova,et al.  Application of quantum dots to multicolor microarray experiments: four-color genotyping. , 2007, Methods in molecular biology.

[9]  S. Gambhir,et al.  Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics , 2005, Science.

[10]  Nobuko Uchida,et al.  Long-term monitoring of transplanted human neural stem cells in developmental and pathological contexts with MRI , 2007, Proceedings of the National Academy of Sciences.

[11]  T. Mihaljevic,et al.  Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping , 2004, Nature Biotechnology.

[12]  Igor L. Medintz,et al.  Quantum dot bioconjugates for imaging, labelling and sensing , 2005, Nature materials.

[13]  Hong Wang,et al.  Detection of tumor marker CA125 in ovarian carcinoma using quantum dots. , 2004, Acta biochimica et biophysica Sinica.

[14]  Qiang Wang,et al.  Synthesis of Extremely Small CdSe and Highly Luminescent CdSe/CdS Core–Shell Nanocrystals via a Novel Two‐Phase Thermal Approach , 2005 .

[15]  Smita Dayal,et al.  Quantum Dot-based Energy Transfer: Perspectives and Potential for Applications in Photodynamic Therapy , 2006, Photochemistry and photobiology.

[16]  Chiming Wei,et al.  Nanomedicine in cancer treatment. , 2005, Nanomedicine : nanotechnology, biology, and medicine.

[17]  James A J Fitzpatrick,et al.  Sentinel lymph node imaging using quantum dots in mouse tumor models. , 2007, Bioconjugate chemistry.

[18]  P. Choyke,et al.  Simultaneous two-color spectral fluorescence lymphangiography with near infrared quantum dots to map two lymphatic flows from the breast and the upper extremity , 2007, Breast Cancer Research and Treatment.

[19]  M. Bawendi,et al.  Renal clearance of quantum dots , 2007, Nature Biotechnology.

[20]  Xiaobo Chen,et al.  Semiconductor quantum dots for photodynamic therapy. , 2003, Journal of the American Chemical Society.

[21]  D. Price,et al.  Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry , 2006, Nature Medicine.

[22]  U. Banin,et al.  Folate-mediated tumor cell uptake of quantum dots entrapped in lipid nanoparticles. , 2007, Journal of controlled release : official journal of the Controlled Release Society.

[23]  Sanjiv S Gambhir,et al.  Self-illuminating quantum dot conjugates for in vivo imaging , 2006, Nature Biotechnology.

[24]  Steven C Kazmierczak,et al.  From diagnostics to therapy: prospects of quantum dots. , 2007, Clinical biochemistry.

[25]  Ron C. Hardman.  A Toxicologic Review of Quantum Dots: Toxicity Depends on Physicochemical and Environmental Factors , 2005, Environmental health perspectives.

[26]  Robert Langer,et al.  Quantum dot-aptamer conjugates for synchronous cancer imaging, therapy, and sensing of drug delivery based on bi-fluorescence resonance energy transfer. , 2007, Nano letters.

[27]  D. Slaaf,et al.  Optical and magnetic resonance imaging of cell death and platelet activation using annexin a5-functionalized quantum dots. , 2007, Nano letters.

[28]  T. Soukka,et al.  Europium nanoparticles and time-resolved fluorescence for ultrasensitive detection of prostate-specific antigen. , 2001, Clinical chemistry.

[29]  Warren C. W. Chan,et al.  Quantum Dots in Biological and Biomedical Research: Recent Progress and Present Challenges , 2006 .

[30]  Igor L. Medintz,et al.  Hydrodynamic dimensions, electrophoretic mobility, and stability of hydrophilic quantum dots. , 2006, The journal of physical chemistry. B.

[31]  Victor S-Y Lin,et al.  A mesoporous silica nanosphere-based carrier system with chemically removable CdS nanoparticle caps for stimuli-responsive controlled release of neurotransmitters and drug molecules. , 2003, Journal of the American Chemical Society.

[32]  John V Frangioni,et al.  Self-illuminating quantum dots light the way , 2006, Nature Biotechnology.

[33]  Prem Gurnani,et al.  Pegylated, steptavidin-conjugated quantum dots are effective detection elements for reverse-phase protein microarrays. , 2005, Bioconjugate chemistry.

[34]  Osman Muhammad,et al.  QUANTUM DOTS ARE PHAGOCYTIZED BY MACROPHAGES AND COLOCALIZE WITH EXPERIMENTAL GLIOMAS , 2007, Neurosurgery.

[35]  M. Bawendi,et al.  (CdSe)ZnS Core-Shell Quantum Dots - Synthesis and Characterization of a Size Series of Highly Luminescent Nanocrystallites , 1997 .

[36]  Sanjiv S Gambhir,et al.  Peptide-labeled near-infrared quantum dots for imaging tumor vasculature in living subjects. , 2006, Nano letters.

[37]  S. Nie,et al.  In vivo cancer targeting and imaging with semiconductor quantum dots , 2004, Nature Biotechnology.

[38]  Noriaki Ohuchi,et al.  In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice. , 2007, Cancer research.

[39]  David Twomey,et al.  Semiautomated multiplexed quantum dot-based in situ hybridization and spectral deconvolution. , 2007, The Journal of molecular diagnostics : JMD.

[40]  S. Nie,et al.  Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules , 2001, Nature Biotechnology.

[41]  P. Hoet,et al.  Nanoparticles – known and unknown health risks , 2004, Journal of nanobiotechnology.

[42]  M. Bruchez,et al.  Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots , 2003, Nature Biotechnology.