SSHSuite: an integrated software package for analysis of large-scale suppression subtractive hybridization data.

Suppression subtractive hybridization (SSH) is a widely used technique for the identification of differentially expressed genes. SSH as well as other types of sequencing projects generate large amounts of anonymous sequences. SSHSuite automates the handling and storage of these sequences and enables identification through similarity searches. SSHSuite also offers analysis tools for the retrieval and comparison of the resulting similarity data. SSHSuite consists of four programs: SSHHandler, SSHOverview, SSHAnalysis, and SSHCompare.

[1]  J. Kapfhammer,et al.  Identification of numerous genes differentially expressed in rat brain during postnatal development by suppression subtractive hybridization and expression analysis of the novel rat gene rMMS2. , 2003, Brain research. Molecular brain research.

[2]  M. Wigler,et al.  Cloning the differences between two complex genomes , 1993, Science.

[3]  P. Green,et al.  Base-calling of automated sequencer traces using phred. I. Accuracy assessment. , 1998, Genome research.

[4]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[5]  Robert D. Finn,et al.  Pfam 3.1: 1313 multiple alignments and profile HMMs match the majority of proteins , 1999, Nucleic Acids Res..

[6]  Gregory D. Schuler,et al.  Database resources of the National Center for Biotechnology Information: update , 2004, Nucleic acids research.

[7]  A. Mescher,et al.  Identification of genes expressed during Xenopus laevis limb regeneration by using subtractive hybridization , 2003, Developmental dynamics : an official publication of the American Association of Anatomists.

[8]  Mi-Ock Lee,et al.  Identification of genes that are induced after cadmium exposure by suppression subtractive hybridization. , 2003, Toxicology.

[9]  P Green,et al.  Base-calling of automated sequencer traces using phred. II. Error probabilities. , 1998, Genome research.

[10]  A. Pardee,et al.  Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. , 1992, Science.

[11]  Aaron P. Campbell,et al.  Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M. Pacyna‐Gengelbach,et al.  Gene expression profiles in human non-small and small-cell lung cancers. , 2003, European journal of cancer.

[13]  S. Minchin,et al.  A DNA expression array to detect toxic stress response in European flounder (Platichthys flesus). , 2003, Aquatic toxicology.

[14]  R. Yolken Subtraction libraries for the molecular characterization of gene-environmental interactions in bipolar disorder. , 2002, Bipolar disorders.