Imaging proliferation in vivo with [F-18]FLT and positron emission tomography

Positron emission tomography (PET) is now regularly used in the diagnosis and staging of cancer. These uses and its ability to monitor treatment response would be aided by the development of imaging agents that can be used to measure tissue and tumor proliferation. We have developed and tested [F-18]FLT (3'-deoxy-3'-fluorothymidine); it is resistant to degradation, is retained in proliferating tissues by the action of thymidine kinase 1 (TK), and produces high-contrast images of normal marrow and tumors in canine and human subjects.

[1]  F Daghighian,et al.  Imaging of brain tumor proliferative activity with iodine-131-iododeoxyuridine. , 1994, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[2]  S. Pauwels,et al.  Production of [2-11C]thymidine for quantification of cellular proliferation with PET. , 1991, International journal of radiation applications and instrumentation. Part A, Applied radiation and isotopes.

[3]  P. Conti,et al.  Synthesis of 2'-fluoro-5-[11C]-methyl-1-beta-D-arabinofuranosyluracil ([11C]-FMAU): a potential nucleoside analog for in vivo study of cellular proliferation with PET. , 1995, Nuclear medicine and biology.

[4]  M. Reivich,et al.  THE [14C]DEOXYGLUCOSE METHOD FOR THE MEASUREMENT OF LOCAL CEREBRAL GLUCOSE UTILIZATION: THEORY, PROCEDURE, AND NORMAL VALUES IN THE CONSCIOUS AND ANESTHETIZED ALBINO RAT 1 , 1977, Journal of neurochemistry.

[5]  H. Kimura,et al.  Fluorine-18-fluorodeoxyglucose and carbon-11-methionine evaluation of lymphadenopathy in sarcoidosis. , 1998, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[6]  G. de Sousa,et al.  Comparative metabolism of 3'-azido-3'-deoxythymidine in cultured hepatocytes from rats, dogs, monkeys, and humans. , 1995, Drug metabolism and disposition: the biological fate of chemicals.

[7]  J. Sherley,et al.  Regulation of human thymidine kinase during the cell cycle. , 1988, The Journal of biological chemistry.

[8]  T. Hickish,et al.  Noninvasive monitoring of tumor metabolism using fluorodeoxyglucose and positron emission tomography in colorectal cancer liver metastases: correlation with tumor response to fluorouracil. , 1996, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[9]  H. Thierens,et al.  Kinetics of [methyl-11C]thymidine in patients with squamous cell carcinoma of the head and neck. , 1996, Acta oncologica.

[10]  C Flexner,et al.  Relationship between plasma concentrations of 3'-deoxy-3'-fluorothymidine (alovudine) and antiretroviral activity in two concentration-controlled trials. , 1994, The Journal of infectious diseases.

[11]  T Ido,et al.  Intratumoral distribution of fluorine-18-fluorodeoxyglucose in vivo: high accumulation in macrophages and granulation tissues studied by microautoradiography. , 1992, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[12]  T. Chou,et al.  Comparisons of anti-human immunodeficiency virus activities, cellular transport, and plasma and intracellular pharmacokinetics of 3'-fluoro-3'-deoxythymidine and 3'-azido-3'-deoxythymidine , 1992, Antimicrobial Agents and Chemotherapy.

[13]  A. Wolf,et al.  18F-5-Fluorouridine, a new probe for measuring the proliferation of tissue in vivo. , 1982, Advances in enzyme regulation.

[14]  M Schwaiger,et al.  Positron emission tomography in non-Hodgkin's lymphoma: assessment of chemotherapy with fluorodeoxyglucose. , 1998, Blood.

[15]  T K Lewellen,et al.  Carbon-11-thymidine and FDG to measure therapy response. , 1998, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[16]  A. Shields,et al.  Development of labeled thymidine analogs for imaging tumor proliferation. , 1996, Nuclear medicine and biology.