A building‐block approach to 3D printing a multichannel, organ‐regenerative scaffold

Multichannel scaffolds, formed by rapid prototyping technologies, retain a high potential for regenerative medicine and the manufacture of complex organs. This study aims to optimize several parameters for producing poly(lactic‐co‐glycolic acid) (PLGA) scaffolds by a low‐temperature, deposition manufacturing, three‐dimensional printing (3DP, or rapid prototyping) system. Concentration of the synthetic polymer solution, nozzle speed and extrusion rate were analysed and discussed. Polymer solution with a concentration of 12% w/v was determined as optimal for formation; large deviation of this figure failed to maintain the desired structure. The extrusion rate was also modified for better construct quality. Finally, several solid organ scaffolds, such as the liver, with proper wall thickness and intact contour were printed. This study gives basic instruction to design and fabricate scaffolds with de novo material systems, particularly by showing the approximation of variables for manufacturing multichannel PLGA scaffolds. Copyright © 2015 John Wiley & Sons, Ltd.

[1]  Brendon M. Baker,et al.  Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues , 2012 .

[2]  Anthony Atala,et al.  Engineering Complex Tissues , 2012, Science Translational Medicine.

[3]  Kai He,et al.  Rapid prototyping of tubular polyurethane and cell/hydrogel constructs: , 2011 .

[4]  Kai He,et al.  Optimizing the fabrication processes for manufacturing a hybrid hierarchical polyurethane–cell/hydrogel construct , 2013 .

[5]  F. Yuan,et al.  A review of three-dimensional in vitro tissue models for drug discovery and transport studies. , 2011, Journal of pharmaceutical sciences.

[6]  A. Khademhosseini,et al.  Microscale technologies for tissue engineering and biology. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[7]  Karl R Edminster,et al.  Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication. , 2009, Biomaterials.

[8]  Wei Xu,et al.  Rapid Prototyping of Polyurethane for the Creation of Vascular Systems , 2008 .

[9]  A. Compere,et al.  Separation of Alcohols from Solution by Lignin Gels , 2008 .

[10]  J A Barron,et al.  Biological Laser Printing: A Novel Technique for Creating Heterogeneous 3-dimensional Cell Patterns , 2004, Biomedical microdevices.

[11]  L L Hench,et al.  Biomaterials: a forecast for the future. , 1998, Biomaterials.

[12]  M. Toner,et al.  Cellular Micropatterns on Biocompatible Materials , 1998, Biotechnology progress.

[13]  Yongnian Yan,et al.  Gelatin-Based Hydrogels for Controlled Cell Assembly , 2010 .

[14]  M. Misra,et al.  Biofibres, biodegradable polymers and biocomposites: An overview , 2000 .

[15]  Wei Xu,et al.  A Polyurethane-Gelatin Hybrid Construct for Manufacturing Implantable Bioartificial Livers , 2008 .

[16]  Anthony Atala,et al.  Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system. , 2012, Biomaterials.

[17]  Xinru Zhao,et al.  In vitro vascularization of a combined system based on a 3D printing technique , 2016, Journal of tissue engineering and regenerative medicine.

[18]  David J Mooney,et al.  VEGF Scaffolds Enhance Angiogenesis and Bone Regeneration in Irradiated Osseous Defects , 2006, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[19]  Joshua M. Pearce,et al.  Building Research Equipment with Free, Open-Source Hardware , 2012, Science.

[20]  Xiaohong Wang,et al.  Overview on Biocompatibilities of Implantable Biomaterials , 2013 .

[21]  Kaija-Stiina Paloheimo,et al.  The Integrations of Biomaterials and Rapid Prototyping Techniques for Intelligent Manufacturing of Complex Organs , 2013 .

[22]  David L Kaplan,et al.  In vitro 3D model for human vascularized adipose tissue. , 2009, Tissue engineering. Part A.

[23]  D Guidolin,et al.  Chondrocyte aggregation and reorganization into three-dimensional scaffolds. , 1999, Journal of biomedical materials research.

[24]  Steve Edmondson,et al.  Design and additive manufacture for flow chemistry. , 2013, Lab on a chip.

[25]  Brian Derby,et al.  Printing and Prototyping of Tissues and Scaffolds , 2012, Science.

[26]  Vladimir Mironov,et al.  Organ printing: computer-aided jet-based 3D tissue engineering. , 2003, Trends in biotechnology.

[27]  Xiaohong Wang,et al.  Application of 3D biomimetic models in drug delivery and regenerative medicine. , 2015, Current pharmaceutical design.

[28]  D. Ingber,et al.  From 3D cell culture to organs-on-chips. , 2011, Trends in cell biology.

[29]  Dai Fukumura,et al.  Engineering vascularized tissue , 2005, Nature Biotechnology.

[30]  K. Burg,et al.  Biomaterial developments for bone tissue engineering. , 2000, Biomaterials.

[31]  Robert Langer,et al.  EDITORIAL: TISSUE ENGINEERING: PERSPECTIVES, CHALLENGES, AND FUTURE DIRECTIONS , 2007 .

[32]  Yongnian Yan,et al.  An cell-assembly derived physiological 3D model of the metabolic syndrome, based on adipose-derived stromal cells and a gelatin/alginate/fibrinogen matrix. , 2010, Biomaterials.

[33]  Xiaohong Wang,et al.  Overview on "Chinese-Finnish workshop on biomanufacturing and evaluation techniques". , 2011, Artificial organs.

[34]  Yuanwen Huang,et al.  Rapid prototyping of a hybrid hierarchical polyurethane-cell/hydrogel construct for regenerative medicine. , 2013, Materials science & engineering. C, Materials for biological applications.

[35]  W. Johnston,et al.  A forecast for the future. , 1983, The Internist.

[36]  Xiaohong Wang,et al.  Recent trends and challenges in complex organ manufacturing. , 2010, Tissue engineering. Part B, Reviews.

[37]  J. Vacanti,et al.  Tissue engineering : Frontiers in biotechnology , 1993 .

[38]  Xiaohong Wang,et al.  Rapid prototyping as a tool for manufacturing bioartificial livers. , 2007, Trends in biotechnology.

[39]  S. Hollister.  Porous scaffold design for tissue engineering , 2005, Nature materials.

[40]  R. Jain.  Normalization of Tumor Vasculature: An Emerging Concept in Antiangiogenic Therapy , 2005, Science.

[41]  Minna Kellomäki,et al.  A review of rapid prototyping techniques for tissue engineering purposes , 2008, Annals of medicine.

[42]  Cheng-Hsien Liu,et al.  Controlled release of growth factors for regenerative medicine. , 2015, Current pharmaceutical design.

[43]  Xinru Zhao,et al.  Preparation of an adipose-derived stem cell/fibrin–poly(d,l-lactic-co-glycolic acid) construct based on a rapid prototyping technique , 2013 .

[44]  Tongkui Cui,et al.  Rapid Prototyping a Double-layer Polyurethane—collagen Conduit and its Schwann Cell Compatibility , 2009 .

[45]  Xiaohong Wang,et al.  Intelligent freeform manufacturing of complex organs. , 2012, Artificial organs.

[46]  Wei Xu,et al.  Rapid prototyping of a double-layer polyurethane-collagen conduit for peripheral nerve regeneration. , 2009, Tissue engineering. Part C, Methods.