论文信息 - Separation of myoglobin and hemoglobin in isolated perfused pig heart

Separation of myoglobin and hemoglobin in isolated perfused pig heart

For the quantitative determination of hemoglobin concentration in heart muscle it is important to distinguish between myoglobin and hemoglobin, two dyes with very similar optical absorption properties. With an isolated perfused pig heart model and EMPHO II SSK we measured tissue spectra in the visible range before and after adding erythrocytes to the perfusate. By calculating light intensity differences we were able to show spatial hemoglobin distribution in heart muscle.

Michael Hiller | Manfred D. Kessler | Tobias Trinks | Robert Rauh

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