EFFECT OF GLYCOSIDASES ON THE FATE OF TRANSFUSED LYMPHOCYTES.

aration which contained 3.7 mg protein per ml,"2 was heated at 450 for 10 min and used for the experiments described in this paper. This fraction, like the preparations described previously by Morgan and his co-workers,'°0 II contains many glycosidases and liberates fucose, galactose, and N-acetylhexosamine from red cell stroma. It also removes the surface sugars of tumor cells as shown histochemically by Gasic and Gasic.13 The activities of various preparations were estimated from their ability to liberate N-acetylhexosamine from red cell stroma under the following conditions: enzyme protein, 0.4 mg, was incubated at 370 with 3 mg (dry weight) of red cell stromal in 0.4 ml of 0.03 M phosphate buffer, pH 7.1. At various times, aliquots were deproteinized' land N-ace-tylhexosamine (measured as N-acetyl-D-glucosamine) in the supernatant solutions determined.'6 The enzyme preparation used in this paper liberated 0.08 /mole of N-acetylhexosamine/hour/ mg of enzyme protein. Other preparations liberated 0.02-0.1 pmole under the same conditions.