A hybrid model combining hydrodynamic and biological effects for production of bacterial cellulose with a pilot scale airlift reactor

Abstract A hybrid model combining hydrodynamic and biological effects is developed to describe the cultivation of Acetobacter xylinum for bacterial cellulose production in a modified airlift reactor with wire-mesh draft tubes. The hydrodynamic part is essentially a modified tanks-in-series model whose parameters are determined using a tracer response method. The modified tanks-in-series model is based on that the materials in the riser and downcomer are exchangeable across the wire-mesh draft tube. In addition, the effect of oxygen transfer is also taken into consideration for the tanks-in-series model. An artificial neural network (ANN) model based on the data of a batch cultivation in a stirred tank reactor combined with system equations is utilized as the biological part. Simulation is carried out to demonstrate that the proposed hybrid model represents the cultivation system very well.

[1]  K. Thayanithy,et al.  A comparison of some methods of estimating volumetric mass‐transfer coefficients in an external‐loop airlift fermenter , 1987, Biotechnology and bioengineering.

[2]  Masatoshi Iguchi,et al.  Bacterial cellulose—a masterpiece of nature's arts , 2000 .

[3]  Campbell W. Robinson,et al.  New criteria for application of the well-mixed model to gas-liquid mass transfer studies , 1983 .

[4]  Wen-Teng Wu,et al.  A Modified Airlift Reactor with High Capabilities of Liquid Mixing and Mass Transfer , 2002 .

[5]  N. Slater,et al.  Tracer dispersion in a laboratory air-lift reactor , 1983 .

[6]  M. Schramm,et al.  Synthesis of cellulose by Acetobacter xylinum. I. Micromethod for the determination of celluloses. , 1954, The Biochemical journal.

[7]  Wen‐Teng Wu,et al.  Cultivation of Acetobacter xylinum for bacterial cellulose production in a modified airlift reactor , 2002, Biotechnology and applied biochemistry.

[8]  Kunihiko Watanabe,et al.  Research Progress in Production of Bacterial Cellulose by Aeration and Agitation Culture and Its Application as a New Industrial Material , 1997 .

[9]  T. Kanai,et al.  Simulation of airlift bioreactors: Steady-state performance of continuous culture processes , 1996 .

[10]  Lyle H. Ungar,et al.  A hybrid neural network‐first principles approach to process modeling , 1992 .

[11]  Y K Yang,et al.  Effects of pH and dissolved oxygen on cellulose production by Acetobacter xylinum BRC5 in agitated culture. , 1999, Journal of bioscience and bioengineering.

[12]  T. Kouda,et al.  Effect of Agitator Configuration on Bacterial Cellulose Productivity in Aerated and Agitated Culture , 1997 .

[13]  Mitsuo Kamiwano,et al.  Characterization of non-newtonian behavior during mixing of bacterial cellulose in a bioreactor , 1996 .

[14]  Rainer Jonas,et al.  Production and application of microbial cellulose , 1998 .

[15]  Aniruddha B. Pandit,et al.  Three Phase Sparged Reactors — Some Design Aspects , 1984 .

[16]  R Mayer,et al.  Cellulose biosynthesis and function in bacteria. , 1991, Microbiological reviews.

[17]  M. Schramm,et al.  Synthesis of cellulose by Acetobacter xylinum. II. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose. , 1954, The Biochemical journal.

[18]  T. Tsuchida,et al.  Screening of Bacterial Cellulose-producing Acetobacter Strains Suitable for Agitated Culture , 1995 .

[19]  K. Schügerl,et al.  Performance of continuous tower loop bioreactors—II model calculations for substrate limited growth , 1982 .

[20]  Yoshinori Kawase,et al.  Dynamic modeling and simulation of continuous airlift bioreactors , 2000 .

[21]  Yu Sam Kim,et al.  Cellulose production by Acetobacter xylinum BRC5 under agitated condition , 1998 .