The radio-frequency dielectric properties of yeast cells measured with a rapid, automated, frequency-domain dielectric spectrometer

Summary o (1) A computerized, rapid-scanning, frequency-domain dielectric spectrometer, capable of operating in the range 5 Hz-13 MHz, and based on commercially available components, is described. (2) Measurements of the passive electrical properties of yeast cells in the range 0.01–13 MHz confirm the well-docomented existence of a pronounced Maxwell-Wagner (β-) dispersion. The use of both complex permittivity (Cole/Cole) and complex conductivity diagrams indicated that dielectric relaxations centred at frequencies somewhat higher than those attainable contribute to the β-dispersion. (3) The data obtained at different concentrations of the suspended phase are compared with the predictions of several suspension equations, some previously untested, and are found to be in excellent accord with them, even at volume fractions as high as 0.4.

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