Effects of pubescence and waxes on the reflectance of leaves in the ultraviolet and photosynthetic wavebands: a comparison of a range of species

Total reflectance of ultraviolet and photosynthetically effective wavelengths was measured for a range of different leaf types. Two approaches were employed. Firstly, reflectance of monochromatic wavebands at 330 and 680 nm was measured for a total of 45 different species covering a wide range of genera. In the second, specific leaf types that displayed different degrees of reflectance were treated to remove hairs and waxes that contributed to their reflectance. Selected waxy and non-waxy leaves were also studied in more detail over the spectral range 270–500 nm. It was found that both pubescence (presence of hairs) and glaucousness (presence of a thick epicuticular wax layer) had marked effects on total reflectance. Pubescent leaves tended to be more effective in reflecting longer wavelengths than ultraviolet radiation. The extent of this effect depended on hair type. Glaucous leaves demonstrated that surface waxes were very effective reflectors of both UV and longer wavelength radiation.

[1]  Further increase in ultraviolet B , 1991, The Lancet.

[2]  Y. Manetas,et al.  Seasonal fluctuations in the concentration of UV-absorbing compounds in the leaves of some Mediterranean plants under field conditions. , 2001, Physiologia plantarum.

[3]  K. Percy Air Pollutants and the Leaf Cuticle , 1994, NATO ASI Series.

[4]  Yiannis Manetas,et al.  Ultraviolet‐B radiation absorbing capacity of leaf hairs , 1992 .

[5]  M. Holmes,et al.  Outdoor ultraviolet polychromatic action spectra for growth responses of Bellis perennis and Cynosurus cristatus. , 2000, Journal of photochemistry and photobiology. B, Biology.

[6]  G. W. Robertson,et al.  Effects of environment on the composition of epicuticular wax esters from kale and swede , 1995 .

[7]  J. Clark,et al.  Photosynthetic Action Spectra of Trees: II. The Relationship of Cuticle Structure to the Visible and Ultraviolet Spectral Properties of Needles from Four Coniferous Species. , 1975, Plant physiology.

[8]  M. G. Holmes,et al.  Effects of long-term exposure to elevated UV-B radiation on the photosynthetic performance of five broad-leaved tree species , 2004, Photosynthesis Research.

[9]  J. Ehleringer,et al.  Ecology and Ecophysiology of Leaf Pubescence in North American Desert Plants , 1984 .

[10]  Stephan D. Flint,et al.  Internal filters: Prospects for UV‐acclimation in higher plants , 1983 .

[11]  A. Wellburn,et al.  The influence of UV-B radiation on the physicochemical nature of tobacco (Nicotiana tabacum L.) leaf surfaces , 1996 .

[12]  G. W. Robertson,et al.  Ecological biochemistryEffects of environment on the composition of epicuticular wax from kale and swede , 1995 .

[13]  Y. Manetas,et al.  Trichome density and its UV-B protective potential are affected by shading and leaf position on the canopy , 1997 .

[14]  A. Webb Plants and UV-B: Monitoring changes in UV-B radiation , 1997 .

[15]  Janet F. Bornman,et al.  The effect of exposure to enhanced UV‐B radiation on the penetration of monochromatic and polychromatic UV‐B radiation in leaves of Brassica napus , 1993 .

[16]  H. Skaltsa,et al.  UV-B PROTECTIVE POTENTIAL AND FLAVONOID CONTENT OF LEAF HAIRS OF QUERCUS ILEX , 1994 .

[17]  Wilhelm Barthlott,et al.  Classification and terminology of plant epicuticular waxes , 1998 .

[18]  M. Tevini,et al.  Influence of Light, UV-B Radiation, and Herbicides on Wax Biosynthesis of Cucumber Seedling , 1987 .

[19]  Martyn M. Caldwell,et al.  Leaf epidermal transmittance of ultraviolet radiation and its implications for plant sensitivity to ulraviolet-radiation induced injury , 2004, Oecologia.

[20]  E. Zvereva,et al.  Effects of leaf pubescence in Salix borealis on host‐plant choice and feeding behaviour of the leaf beetle, Melasoma lapponica , 1998 .

[21]  A. Wellburn,et al.  Effects of UV-B Radiation on Wax Biosynthesis , 1994 .

[22]  D. Oosterhuis,et al.  Effect of water stress on the epicuticular wax composition and ultrastructure of cotton (Gossypium hirsutum L.) leaf, bract, and boll , 1996 .

[23]  M. Holmes Plants and UV-B: Action spectra for UV-B effects on plants: monochromatic and polychromatic approaches for analysing plant responses , 1997 .

[24]  H. N. Barber ADAPTIVE GENE SUBSTITUTIONS IN TASMANIAN EUCALYPTS: I. GENES CONTROLLING THE DEVELOPMENT OF GLAUCOUSNESS , 1955 .

[25]  Martyn M. Caldwell,et al.  Leaf Ultraviolet Optical Properties Along a Latitudinal Gradient in the Arctic‐Alpine Life Zone , 1980 .

[26]  E. DeLucia,et al.  Are some plant life forms more effective than others in screening out ultraviolet-B radiation? , 1992, Oecologia.

[27]  M. Tevini,et al.  Action of ultraviolet radiation (UV-B) upon cuticular waxes in some crop plants , 1985, Planta.

[28]  J. Farman,et al.  Large losses of total ozone in Antarctica reveal seasonal ClOx/NOx interaction , 1985, Nature.

[29]  A. J. Miller,et al.  Record Low Global Ozone in 1992 , 1993, Science.

[30]  Yiannis Manetas,et al.  Leaf hairs of Olea europeae protect underlying tissues against ultraviolet-B radiation damage , 1993 .

[31]  R. N. Kickert,et al.  Elevated ultraviolet (UV)-B radiation and agriculture , 1998 .

[32]  J. Kerr,et al.  Evidence for Large Upward Trends of Ultraviolet-B Radiation Linked to Ozone Depletion , 1993, Science.

[33]  David Rind,et al.  Increased polar stratospheric ozone losses and delayed eventual recovery owing to increasing greenhouse-gas concentrations , 1998, Nature.

[34]  M. Caldwell,et al.  The changing solar ultraviolet climate and the ecological consequences for higher plants. , 1989, Trends in Ecology & Evolution.

[35]  M. Holmes,et al.  Growth and stomatal responses of temperate meadow species to enhanced levels of UV-A and UV-B+A radiation in the natural environment. , 2000, Journal of photochemistry and photobiology. B, Biology.

[36]  M. Molina,et al.  Stratospheric sink for chlorofluoromethanes: chlorine atomc-atalysed destruction of ozone , 1974, Nature.

[37]  J. Bornman,et al.  Effects of Ultraviolet-B Radiation on Terrestrial Plants , 1993 .

[38]  Markus Riederer,et al.  Attenuation of UV radiation by plant cuticles from woody species , 1997 .

[39]  C. Cockell,et al.  Ultraviolet radiation screening compounds , 1999, Biological reviews of the Cambridge Philosophical Society.

[40]  K. Oyama,et al.  Variation in leaf trichomes of Wigandia urens: environmental factors and physiological consequences. , 2000, Tree physiology.

[41]  A. Teramura,et al.  Effects of UV-B radiation on photosynthesis and growth of terrestrial plants , 1994, Photosynthesis Research.

[42]  T. Mulroy,et al.  Spectral properties of heavily glaucous and non-glaucous leaves of a succulent rosette-plant , 2004, Oecologia.

[43]  K. Percy,et al.  Effect of UV-B Dose on Biosytnthesis of Epicuticular Waxes in Blue Spruce (Picea pungens Engelmann.) Primary Needles: Preliminary Investigation , 1999 .

[44]  S. Britz,et al.  Inhibitory effects of ambient levels of solar UV‐A and UV‐B radiation on growth of cucumber , 1997 .

[45]  Thomas C. Vogelmann,et al.  Penetration of blue nad UV radiation measured by fiber optics in spruce and fir needles , 1988 .

[46]  L. Björn,et al.  UV-B as an environmental factor in plant life: stress and regulation. , 1997, Trends in ecology & evolution.

[47]  D. Hofmann,et al.  Evidence from balloon measurements for chemical depletion of stratospheric ozone in the Arctic winter of 1989–90 , 1991, Nature.

[48]  R. Biggs,et al.  Some Morphological and Biochemical Characteristics of C3 and C4 Plants Irradiated with UV‐B , 1978 .

[49]  M. Blumthaler,et al.  Indication of increasing solar ultraviolet-B radiation flux in alpine regions. , 1990, Science.

[50]  Nigel D. Paul,et al.  Responses to ultraviolet-B radiation (280-315 nm) of pea (Pisum sativum) lines differing in leaf surface wax , 1996 .

[51]  Josep Peñuelas,et al.  Altitudinal differences in UV absorbance, UV reflectance and related morphological traits of Quercus ilex and Rhododendron ferrugineum in the Mediterranean region , 1999, Plant Ecology.

[52]  A. Bais,et al.  Variability of UV‐B at four stations in Europe , 1997 .

[53]  Baker,et al.  Ultraviolet-B radiation effects on water relations, leaf development, and photosynthesis in droughted pea plants , 1998, Plant physiology.

[54]  Park S. Nobel,et al.  Biophysical plant physiology and ecology , 1983 .

[55]  M. Seyfried 9 – Optical Radiation Interactions with Living Tissue , 1989 .