Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner

[1]  T. Arakawa,et al.  Halophilic characterization of starch-binding domain from Kocuria varians α-amylase. , 2012, International journal of biological macromolecules.

[2]  J. Chon,et al.  Advances in the production and downstream processing of antibodies. , 2011, New biotechnology.

[3]  D. Ejima,et al.  Refolding single-chain antibody (scFv) using lauroyl-L-glutamate as a solubilization detergent and arginine as a refolding additive. , 2011, Protein expression and purification.

[4]  Taketo Yamada Therapeutic monoclonal antibodies. , 2011, The Keio journal of medicine.

[5]  T. Arakawa,et al.  Salt-dependent thermo-reversible α-amylase: cloning and characterization of halophilic α-amylase from moderately halophilic bacterium, Kocuria varians , 2011, Applied Microbiology and Biotechnology.

[6]  T. Arakawa,et al.  High solubility supports efficient refolding of thermally unfolded β-lactamase. , 2010, International journal of biological macromolecules.

[7]  T. Arakawa,et al.  Novel soluble expression technologies derived from unique properties of halophilic proteins , 2010, Applied Microbiology and Biotechnology.

[8]  Christian Bailly,et al.  Strategies and challenges for the next generation of therapeutic antibodies , 2010, Nature Reviews Immunology.

[9]  Abhinav A Shukla,et al.  Recent advances in large-scale production of monoclonal antibodies and related proteins. , 2010, Trends in biotechnology.

[10]  R. Kontermann Alternative antibody formats. , 2010, Current opinion in molecular therapeutics.

[11]  H. Hanagata,et al.  Brevibacillus expression system: host-vector system for efficient production of secretory proteins. , 2010, Current pharmaceutical biotechnology.

[12]  T. Arakawa,et al.  Halophilic β-lactamase as a new solubility- and folding-enhancing tag protein: production of native human interleukin 1α and human neutrophil α-defensin , 2010, Applied Microbiology and Biotechnology.

[13]  F. Brovko,et al.  Refolding of scFv mini-antibodies using size-exclusion chromatography via arginine solution layer. , 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[14]  M. Thiel,et al.  Efficient intraocular penetration of topical anti-TNF-alpha single-chain antibody (ESBA105) to anterior and posterior segment without penetration enhancer. , 2009, Investigative ophthalmology & visual science.

[15]  Scott M Glaser,et al.  Antibody therapeutics, antibody engineering, and the merits of protein stability. , 2008, Current opinion in drug discovery & development.

[16]  Hiroko Tokunaga,et al.  Engineering of halophilic enzymes: Two acidic amino acid residues at the carboxy‐terminal region confer halophilic characteristics to Halomonas and Pseudomonas nucleoside diphosphate kinases , 2008, Protein science : a publication of the Protein Society.

[17]  T. Ohkuri,et al.  Stable supply of large amounts of human Fab from the inclusion bodies in E. coli. , 2007, Journal of biochemistry.

[18]  T. Arakawa,et al.  Dimeric structure of nucleoside diphosphate kinase from moderately halophilic bacterium: contrast to the tetrameric Pseudomonas counterpart. , 2007, FEMS microbiology letters.

[19]  L. Weiner,et al.  Antibody constructs in cancer therapy , 2007, Cancer.

[20]  T. Arakawa,et al.  Contribution of halophilic nucleoside diphosphate kinase sequence to the heat stability of chimeric molecule. , 2006, Protein and peptide letters.

[21]  P. DasSarma,et al.  Post-genomics of the model haloarchaeon Halobacterium sp. NRC-1 , 2006, Saline systems.

[22]  H. Fukada,et al.  Opposing effects of NaCl on reversibility and thermal stability of halophilic beta-lactamase from a moderate halophile, Chromohalobacter sp. 560. , 2006, Biophysical chemistry.

[23]  D. Ejima,et al.  Arginine as an effective additive in gel permeation chromatography. , 2005, Journal of chromatography. A.

[24]  B Tidor,et al.  Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. , 2004, Journal of molecular biology.

[25]  D. Reilly,et al.  Production technologies for monoclonal antibodies and their fragments. , 2004, Current opinion in biotechnology.

[26]  T. Arakawa,et al.  Highly efficient renaturation of β‐lactamase isolated from moderately halophilic bacteria , 2004, FEBS letters.

[27]  M. Andersson,et al.  Accuracy in multiangle light scattering measurements for molar mass and radius estimations. Model calculations and experiments. , 2003, Analytical chemistry.

[28]  L. Stockwin,et al.  The role of therapeutic antibodies in drug discovery. , 2003, Biochemical Society transactions.

[29]  Kouhei Tsumoto,et al.  Practical considerations in refolding proteins from inclusion bodies. , 2003, Protein expression and purification.

[30]  Robert J. Moore,et al.  High-Level Production of Recombinant Chicken Interferon-γ by Brevibacillus choshinensis , 2001 .

[31]  Humphreys Dp,et al.  Therapeutic antibody production technologies: molecules, applications, expression and purification. , 2001 .

[32]  A. Plückthun,et al.  Stability engineering of antibody single-chain Fv fragments. , 2001, Journal of molecular biology.

[33]  D. Humphreys,et al.  Therapeutic antibody production technologies: molecules, applications, expression and purification. , 2001, Current opinion in drug discovery & development.

[34]  F. Frolow,et al.  Halophilic enzymes: proteins with a grain of salt. , 2000, Biophysical chemistry.

[35]  E Schwarz,et al.  Advances in refolding of proteins produced in E. coli. , 1998, Current opinion in biotechnology.

[36]  K. Tsumoto,et al.  Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment. , 1998, Journal of immunological methods.

[37]  J A McCammon,et al.  Electrostatic contributions to the stability of halophilic proteins. , 1998, Journal of molecular biology.

[38]  Antonio Ventosa,et al.  Biology of Moderately Halophilic Aerobic Bacteria , 1998, Microbiology and Molecular Biology Reviews.

[39]  D. Segal,et al.  Correct disulfide pairing and efficient refolding of detergent-solubilized single-chain Fv proteins from bacterial inclusion bodies. , 1995, Molecular immunology.

[40]  K. Tsumoto,et al.  Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties. , 1994, Biochemical and biophysical research communications.

[41]  S. Udaka,et al.  Screening and Characterization of Protein-hyperproducing Bacteria without Detectable Exoprotease Activity , 1989 .

[42]  P. K. Smith,et al.  Measurement of protein using bicinchoninic acid. , 1985, Analytical biochemistry.

[43]  U. K. Laemmli,et al.  Cleavage of structural proteins during , 1970 .