Peptides for functionalization of InP semiconductors.

The challenge is to achieve high specificity in molecular sensing by proper functionalization of micro/nano-structured semiconductors by peptides that reveal specific recognition for these structures. Here we report on surface modification of the InP semiconductors by adhesion peptides produced by the phage display technique. An M13 bacteriophage library has been used to screen 10(10) different peptides against the InP(001) and the InP(111) surfaces to finally isolate specific peptides for each orientation of the InP. MALDI-TOF/TOF mass spectrometry has been employed to study real affinity of the peptide towards the InP surfaces. The peptides serve for controlled placement of biotin onto InP to bind then streptavidin. Our Atomic Force Microscopy study revealed a total surface coverage of molecules when the InP surface was functionalized by its specific biotinylated peptide (YAIKGPSHFRPS). Finally, fluorescence microscopy has been employed to demonstrate the preferential attachment of the peptide onto a micro-patterned InP surface. Use of substrate specific peptides could present an alternative solution for the problems encountered in the actually existing sensing methods and molecular self-assembly due to the unwanted unspecific interactions.

[1]  D. Wright,et al.  Monoclonal Antibody Recognition of Histidine-Rich Peptide Encapsulated Nanoclusters , 2002 .

[2]  Claudine Boiziau,et al.  Selection by Phage Display of Peptides Targeting the HIV-1 TAR Element , 2005, RNA biology.

[3]  K. Schulten,et al.  Molecular biomimetics: nanotechnology through biology , 2003, Nature materials.

[4]  Moungi G Bawendi,et al.  Oligomeric ligands for luminescent and stable nanocrystal quantum dots. , 2003, Journal of the American Chemical Society.

[5]  Jeffrey J. Gray,et al.  The interaction of proteins with solid surfaces. , 2004, Current opinion in structural biology.

[6]  S. Pathak,et al.  Hydroxylated quantum dots as luminescent probes for in situ hybridization. , 2001, Journal of the American Chemical Society.

[7]  Jeffrey A. Hubbell,et al.  Functional biomaterials : Design of novel biomaterials : Biomaterials , 2001 .

[8]  B. Braden,et al.  X-ray crystal structure of an anti-Buckminsterfullerene antibody fab fragment: biomolecular recognition of C(60). , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[9]  C. J. Murray,et al.  Peptide Templates for Nanoparticle Synthesis Derived from Polymerase Chain Reaction‐Driven Phage Display , 2004 .

[10]  Shimon Weiss,et al.  Bioactivation and cell targeting of semiconductor CdSe/ZnS nanocrystals with phytochelatin-related peptides. , 2004, Journal of the American Chemical Society.

[11]  Zhivko Zhelev,et al.  Quantum dots as photosensitizers? , 2004, Nature Biotechnology.

[12]  S. Nie,et al.  Quantum dot bioconjugates for ultrasensitive nonisotopic detection. , 1998, Science.

[13]  Candan Tamerler,et al.  Molecular biomimetics: utilizing nature's molecular ways in practical engineering. , 2007, Acta biomaterialia.

[14]  Bettina Schmitt,et al.  Selection of D‐Amino‐Acid Peptides That Bind to Alzheimer's Disease Amyloid Peptide Aβ1–42 by Mirror Image Phage Display , 2003, Chembiochem : a European journal of chemical biology.

[15]  S. Brown,et al.  Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[16]  R. Doolittle,et al.  A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.

[17]  K. Oda,et al.  Toxicity of a low level of indium phosphide (InP) in rats after intratracheal instillation. , 1997, Industrial health.

[18]  Stephen Todryk,et al.  A novel method to identify and characterise peptide mimotopes of heat shock protein 70-associated antigens , 2006, Journal of immune based therapies and vaccines.

[19]  Xiaogang Peng,et al.  Conjugation Chemistry and Bioapplications of Semiconductor Box Nanocrystals Prepared via Dendrimer Bridging , 2003 .

[20]  C. Niemeyer.  REVIEW Nanoparticles, Proteins, and Nucleic Acids: Biotechnology Meets Materials Science , 2022 .

[21]  Milan Mrksich,et al.  Self-assembled monolayers of alkanethiolates presenting mannitol groups are inert to protein adsorption and cell attachment , 2000 .

[22]  Zoltán Bálint,et al.  Tailoring GaN semiconductor surfaces with biomolecules. , 2008, The journal of physical chemistry. B.

[23]  Erica Anderson,et al.  Identification of polypeptides with selective affinity to intact mouse cerebellar granule neurons from a random peptide-presenting phage library , 2004, Journal of Neuroscience Methods.

[24]  J. Matthew Mauro,et al.  Self-Assembly of CdSe−ZnS Quantum Dot Bioconjugates Using an Engineered Recombinant Protein , 2000 .