Typing of Nosocomial Outbreaks of Acinetobacter baumannii by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been evaluated for the identification of multidrug-resistant Acinetobacter baumannii nosocomial outbreaks in comparison with the repetitive sequence-based PCR DiversiLab system. The results suggest that MALDI-TOF MS can be used for real-time detection of Acinetobacter outbreaks before results from DNA-based systems are available.

[1]  E Bergogne-Bérézin,et al.  Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features , 1996, Clinical microbiology reviews.

[2]  R. Bonomo,et al.  Interlaboratory reproducibility of DiversiLab rep-PCR typing and clustering of Acinetobacter baumannii isolates. , 2012, Journal of medical microbiology.

[3]  P. Liu,et al.  Use of different PCR-based DNA fingerprinting techniques and pulsed-field gel electrophoresis to investigate the epidemiology of Acinetobacter calcoaceticus-Acinetobacter baumannii complex. , 1997, Diagnostic microbiology and infectious disease.

[4]  J. Lay,et al.  Experimental factors affecting the quality and reproducibility of MALDI TOF mass spectra obtained from whole bacteria cells , 2003, Journal of the American Society for Mass Spectrometry.

[5]  F. Rodríguez-Valera,et al.  Development of a Multilocus Sequence Typing Scheme for Characterization of Clinical Isolates of Acinetobacter baumannii , 2005, Journal of Clinical Microbiology.

[6]  Harald Seifert,et al.  Acinetobacter baumannii: Emergence of a Successful Pathogen , 2008, Clinical Microbiology Reviews.

[7]  M. Zervos,et al.  The role of molecular biology and nucleic Acid technology in the study of human infection and epidemiology. , 2003, Archives of pathology & laboratory medicine.

[8]  Martin Aepfelbacher,et al.  MALDI-TOF MS fingerprinting allows for discrimination of major methicillin-resistant Staphylococcus aureus lineages. , 2011, International journal of medical microbiology : IJMM.

[9]  D H Persing,et al.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing , 1995, Journal of clinical microbiology.

[10]  Mercedes Treviño,et al.  Molecular and epidemiological analysis of nosocomial carbapenem-resistant Klebsiella spp. using repetitive extragenic palindromic-polymerase chain reaction and matrix-assisted laser desorption/ionization-time of flight. , 2011, Microbial drug resistance.

[11]  G. Fadda,et al.  Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to rpoB gene sequencing for species identification of bloodstream infection staphylococcal isolates. , 2011, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[12]  J. Lay,et al.  MALDI-TOF mass spectrometry of bacteria. , 2001, Mass spectrometry reviews.

[13]  J. Llovo,et al.  Susceptibility trends of Bacteroides fragilis group and characterisation of carbapenemase-producing strains by automated REP-PCR and MALDI TOF. , 2012, Anaerobe.

[14]  Gregor Tanner,et al.  Determining Confidence Intervals When Measuring Genetic Diversity and the Discriminatory Abilities of Typing Methods for Microorganisms , 2001, Journal of Clinical Microbiology.

[15]  G. Sensabaugh,et al.  Clonal Characterization of Staphylococcus aureus by Multilocus Restriction Fragment Typing, a Rapid Screening Approach for Molecular Epidemiology , 2003, Journal of Clinical Microbiology.

[16]  C. Vandenbroucke-Grauls,et al.  Comparison of Amplified Ribosomal DNA Restriction Analysis, Random Amplified Polymorphic DNA Analysis, and Amplified Fragment Length Polymorphism Fingerprinting for Identification ofAcinetobacter Genomic Species and Typing ofAcinetobacter baumannii , 1998, Journal of Clinical Microbiology.

[17]  A. Mellmann,et al.  Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in Comparison to 16S rRNA Gene Sequencing for Species Identification of Nonfermenting Bacteria , 2008, Journal of Clinical Microbiology.

[18]  A. Antoniadou,et al.  Acinetobacter baumannii: a universal threat to public health? , 2008, International journal of antimicrobial agents.

[19]  G. Kleinheinz,et al.  Discrimination and characterization of environmental strains of Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). , 2007, Journal of microbiological methods.

[20]  A. Fluit,et al.  Evaluation of the DiversiLab System for Detection of Hospital Outbreaks of Infections by Different Bacterial Species , 2010, Journal of Clinical Microbiology.

[21]  K. Towner.  Acinetobacter: an old friend, but a new enemy. , 2009, The Journal of hospital infection.

[22]  Etienne Carbonnelle,et al.  MALDI-TOF mass spectrometry tools for bacterial identification in clinical microbiology laboratory. , 2011, Clinical biochemistry.

[23]  Helen Sutton,et al.  Compilation of a MALDI-TOF mass spectral database for the rapid screening and characterisation of bacteria implicated in human infectious diseases. , 2004, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[24]  P. Higgins,et al.  Molecular Epidemiology of Acinetobacter baumannii Bloodstream Isolates Obtained in the United States from 1995 to 2004 Using rep-PCR and Multilocus Sequence Typing , 2012, Journal of Clinical Microbiology.

[25]  M. Yoshino,et al.  Rapid discrimination of Legionella by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. , 2011, Microbiological research.

[26]  G. Prévost,et al.  Rapid discrimination of environmental Vibrio by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. , 2012, Microbiological research.

[27]  J A Carriço,et al.  Illustration of a Common Framework for Relating Multiple Typing Methods by Application to Macrolide-Resistant Streptococcus pyogenes , 2006, Journal of Clinical Microbiology.

[28]  P. Hunter,et al.  Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity , 1988, Journal of clinical microbiology.