Seroepidemiological Study of Canine and Human Dirofilariasis in the Endemic Region of Northern Serbia

Dirofilariasis is a vector-borne zoonotic disease caused mainly by Dirofilaria immitis and Dirofilaria repens that affect dogs and humans all over the world. Serbia is considered an endemic country to both forms of dirofilariasis, although most of the population is concentrated in the north of the country. The aims of this study were to show the prevalence of D. immitis and D. repens in dogs and the seroprevalence in humans compared to previous studies in Northern Serbia. In total, 346 dog sera samples and 265 human samples were analyzed. Dog blood samples were analyzed using the modified Knott's method to check whether there were Dirofilaria spp. microfilariae and serum samples were checked by a commercial D. immitis antigen test. Human serum samples were analyzed with a non-commercial ELISA for detection of specific anti-D. immitis, anti-D. repens, and anti-Wolbachia IgG antibodies, and confirmed by western blotting. The overall prevalence for Dirofilaria spp. in dogs was 29.19%. The overall prevalence for D. immitis was 26.30%. The percentages of D. immitis and D. repens microfilaremia in dogs were 25.72 and 1.45%, respectively, while D. immitis./D. repens microfilaremia co-infections were also 1.45%. The overall seroprevalence for Dirofilaria spp. in humans was 3.77%. The overall seroprevalence for D. immitis was 1.51, 1.13% for D. repens, and for D. immitis/D. repens co-infections was 1.13%. The results indicate that D. immitis and D. repens are present in dogs and humans in the province of Vojvodina, in the northern part of Serbia. It is most likely associated with the presence of many rivers, the climate, and presence of mosquitoes in the area, so there could be a real public health risk.

[1]  S. Savić,et al.  Molecular survey of Dirofilaria species in stray dogs, red foxes and golden jackals from Vojvodina, Serbia. , 2019, Comparative immunology, microbiology and infectious diseases.

[2]  R. Farkas,et al.  The current situation of canine dirofilariosis in Hungary , 2019, Parasitology Research.

[3]  A. Fontes-Sousa,et al.  Exposure of humans to the zoonotic nematode Dirofilaria immitis in Northern Portugal , 2019, Epidemiology and Infection.

[4]  I. Tsachev,et al.  Human Dirofilariasis in Bulgaria Between 2009 and 2018 , 2019, Helminthologia.

[5]  A. Paulauskas,et al.  Dirofilaria repens in dogs and humans in Lithuania , 2019, Parasites & Vectors.

[6]  D. Otranto,et al.  Recent advances on Dirofilaria repens in dogs and humans in Europe , 2018, Parasites & Vectors.

[7]  L. Rinaldi,et al.  Seroepidemiological survey of human exposure to Dirofilaria spp. in Romania and Moldova. , 2018, Acta tropica.

[8]  V. Čabanová,et al.  Heartworm on the rise—new insights into Dirofilaria immitis epidemiology , 2018, Parasitology Research.

[9]  J. A. Montoya-Alonso,et al.  The Canary Islands as a model of risk of pulmonary dirofilariasis in a hyperendemic area , 2018, Parasitology Research.

[10]  C. Genchi,et al.  Subcutaneous dirofilariosis (Dirofilaria repens): an infection spreading throughout the old world , 2017, Parasites & Vectors.

[11]  A. Mihalca,et al.  Filarioid infections in wild carnivores: a multispecies survey in Romania , 2017, Parasites & Vectors.

[12]  J. González-Miguel,et al.  Current status of canine dirofilariosis in an endemic area of western Spain , 2017, Journal of Helminthology.

[13]  S. Savić,et al.  An appraisal of canine and human cases reveals an endemic status of dirofilariosis in parts of Serbia. , 2017, Molecular and cellular probes.

[14]  L. Kosić,et al.  DIROFILARIOSIS AND ANGIOSTRONGILOSIS IN PET AND HUNTING DOGS IN NOVI SAD, VOJVODINA, SERBIA , 2017 .

[15]  K. Bányai,et al.  First molecular identification of Dirofilaria spp. (Onchocercidae) in mosquitoes from Serbia , 2016, Parasitology Research.

[16]  J. González-Miguel,et al.  Human subcutaneous/ocular dirofilariasis in the Russian Federation and Belarus, 1997-2013. , 2015, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[17]  J. González-Miguel,et al.  Thirty cases of human subcutaneous dirofilariasis reported in Rostov-on-Don (Southwestern Russian Federation). , 2015, Enfermedades infecciosas y microbiologia clinica.

[18]  D. Ćirović,et al.  First records of Dirofilaria repens in wild canids from the region of Central Balkan. , 2014, Acta veterinaria Hungarica.

[19]  J. González-Miguel,et al.  Geo-environmental model for the prediction of potential transmission risk of Dirofilaria in an area with dry climate and extensive irrigated crops. The case of Spain. , 2014, Veterinary parasitology.

[20]  Z. Milošević,et al.  Seroreactivity to Dirofilaria antigens in people from different areas of Serbia , 2014, BMC Infectious Diseases.

[21]  J. González-Miguel,et al.  Delayed Diagnosis of Dirofilariasis and Complex Ocular Surgery, Russia , 2013, Emerging infectious diseases.

[22]  A. Ignjatović,et al.  Canine Dirofilaria infections in two uninvestigated areas of Serbia: epidemiological and genetic aspects. , 2012, Vector borne and zoonotic diseases.

[23]  J. González-Miguel,et al.  Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic , 2012, Clinical Microbiology Reviews.

[24]  L. Rossi,et al.  Subcutaneous Dirofilariosis in South‐East Serbia‐Case Report , 2011, Zoonoses and public health.

[25]  V. Kartashev,et al.  Canine and Human Dirofilariosis in the Rostov Region (Southern Russia) , 2011, Veterinary medicine international.

[26]  V. Arsić-Arsenijević,et al.  Human Dirofilaria repens infection in Serbia , 2009, Journal of Helminthology.

[27]  L. Kramer,et al.  Wolbachia in Dirofilaria repens, an Agent Causing Human Subcutaneous Dirofilariasis , 2008, The Journal of parasitology.

[28]  L. Rossi,et al.  Survey of canine dirofilariasis in Vojvodina, Serbia , 2008, Parasitology Research.

[29]  C. Genchi,et al.  Guideline for the laboratory diagnosis of canine and feline Dirofilaria infections. , 2007 .

[30]  C. Genchi,et al.  Immunopathology of Dirofilaria immitis Infection , 2007, Veterinary Research Communications.

[31]  L. Kramer,et al.  Immune response to and tissue localization of the Wolbachia surface protein (WSP) in dogs with natural heartworm (Dirofilaria immitis) infection. , 2005, Veterinary immunology and immunopathology.

[32]  V. Arsić-Arsenijević,et al.  Subcutaneous Dirofilaria repens infection of the eyelid in Serbia and Montenegro. , 2004, Parasite.

[33]  C. Bandi,et al.  Immunoglobulin G Antibodies against the Endosymbionts of Filarial Nematodes (Wolbachia) in Patients with Pulmonary Dirofilariasis , 2003, Clinical Diagnostic Laboratory Immunology.

[34]  A. Muro,et al.  Utility of antibodies against a 22 kD molecule of Dirofilaria immitis in the diagnosis of human pulmonary dirofilariasis , 1998, Tropical medicine & international health : TM & IH.

[35]  C. Genchi,et al.  Human humoral immune response to Dirofilaria species. , 1997, Parassitologia.

[36]  A. Muro,et al.  Evaluation of Dirofilaria immitis excretory/secretory products for seroepidemiological studies on human dirofilariosis. , 1995, Parasite.

[37]  A. Muro,et al.  Evaluation of a 22 kDa Dirofilaria immitis antigen for the immunodiagnosis of human pulmonary dirofilariosis. , 1994, Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit.

[38]  A. Muro,et al.  A seroepidemiologic survey of human dirofilariosis in Western Spain. , 1991, Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit.

[39]  Kraus Jf,et al.  Combination of filtration and histochemical stain for detection and differentiation of Dirofilaria immitis and Dipetalonema reconditum in the dog. , 1981 .

[40]  J. Kraus,et al.  Combination of filtration and histochemical stain for detection and differentiation of Dirofilaria immitis and Dipetalonema reconditum in the dog. , 1981, American journal of veterinary research.