Potential value of Ginkgolide B on nervous autoimmune disease

Objectives: Inducted rates of bone marrow mesenchymal stem cells (BMSCs) have been used to replace old or damaged cells as stem cells, and treat human immune system conditions or cells that are hard to self-repair. Immunologic characteristics and the transformation tendency of inducted cells are especially important for the final success of a treatment. This preliminary study extracted bilobalide B from ginkgo leaves, and studied the immunologic characteristics and system of inducted cells to determine the most suitable seed cells for the clinical treatment of neurological autoimmune diseases (e.g. Guillain-Barré syndrome). This could be used as a substitute in the regulation and rehabilitation of a damaged immune system caused by neurologic defects and nerve damage. Methods: Cellular morphological changes in ginkgolide B (GB)-inducted cells were observed using an inverted phase contrast microscope at several time points. Inducted cells were stained with immunofluorescence to electrophysiologically estimate the rate of GB induction in BMSCs at different time points before or after induction using the patch clamp technique and whole-cell recording method. Results: In induction extension, differentiated nerve-like cells gradually shaped into neurons at neuronal proportions. Membrane potential in time variations were: peak current of undifferentiated cells (1.45 ± 0.15 PA/PF, n=10), and peak current of inducted neurons (2.18 ± 0.14 PA/PF, n=12); and this exhibited a remarkable variation (P<0.05). Conclusions: GB-inducted BMSCs in rats morphologically changed earlier than the membrane potential. Potential changes in the membrane were more specific with induction time, which reflect cell differentiation. Nerve-like cells inducted by bilobalide B with neural stem cell (NSC) immunology can seed cells with immunity to regulate and restore nerves.