Expression Screening of Integral Membrane Proteins by Fusion to Fluorescent Reporters.

[1]  E. Gouaux,et al.  Neurotransmitter and psychostimulant recognition by the dopamine transporter , 2015, Nature.

[2]  D. Veprintsev,et al.  Large-scale production and protein engineering of G protein-coupled receptors for structural studies , 2015, Front. Pharmacol..

[3]  C. Hélix-Nielsen,et al.  High yield purification of full-length functional hERG K+ channels produced in Saccharomyces cerevisiae , 2015, Microbial Cell Factories.

[4]  R. Owens,et al.  Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli , 2015, Journal of visualized experiments : JoVE.

[5]  R. Owens,et al.  GFP-based expression screening of membrane proteins in insect cells using the baculovirus system. , 2015, Methods in molecular biology.

[6]  Edmund R. S. Kunji,et al.  Membrane protein expression in Lactococcus lactis. , 2015, Methods in enzymology.

[7]  C. Tate,et al.  Quality Control in Eukaryotic Membrane Protein Overproduction , 2014, Journal of molecular biology.

[8]  Mark S.P. Sansom,et al.  Crystal structure of the sodium–proton antiporter NhaA dimer and new mechanistic insights , 2014, The Journal of general physiology.

[9]  E. Gouaux,et al.  Screening and large-scale expression of membrane proteins in mammalian cells for structural studies , 2014, Nature Protocols.

[10]  Anna Hjelm,et al.  MemStar: A one‐shot Escherichia coli‐based approach for high‐level bacterial membrane protein production , 2014, FEBS letters.

[11]  D. Doyle,et al.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction of a lysine-specific permease from Pseudomonas aeruginosa. , 2014, Acta crystallographica. Section F, Structural biology communications.

[12]  Surajit Banerjee,et al.  X-ray structures of GluCl in apo states reveal a gating mechanism of Cys-loop receptors , 2014, Nature.

[13]  N. Yan,et al.  The recombinant expression systems for structure determination of eukaryotic membrane proteins , 2014, Protein & Cell.

[14]  E. Gouaux,et al.  Structure and Dynamics of AMPA Receptor GluA2 in Resting, Pre-Open, and Desensitized States , 2014, Cell.

[15]  Anna Hjelm,et al.  Bacterial-based membrane protein production. , 2014, Biochimica et biophysica acta.

[16]  Xianqiang Song,et al.  NMDA receptor structures reveal subunit arrangement and pore architecture , 2014, Nature.

[17]  E. Gouaux,et al.  X-Ray Structure of Acid-Sensing Ion Channel 1–Snake Toxin Complex Reveals Open State of a Na+-Selective Channel , 2014, Cell.

[18]  B. Poolman,et al.  Evolved Escherichia coli strains for amplified, functional expression of membrane proteins. , 2014, Journal of molecular biology.

[19]  P. A. Pedersen,et al.  Saccharomyces cerevisiae–Based Platform for Rapid Production and Evaluation of Eukaryotic Nutrient Transporters and Transceptors for Biochemical Studies and Crystallography , 2013, PloS one.

[20]  R. Tampé,et al.  Multicolour Fluorescence-Detection Size-Exclusion Chromatography for Structural Genomics of Membrane Multiprotein Complexes , 2013, PloS one.

[21]  R. Owens,et al.  Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology. , 2013, Current opinion in structural biology.

[22]  C. Brooks,et al.  Rapid expression screening of eukaryotic membrane proteins in Pichia pastoris , 2013, Protein science : a publication of the Protein Society.

[23]  Xin Huang,et al.  Rapid screening of membrane protein expression in transiently transfected insect cells. , 2013, Protein expression and purification.

[24]  D. Drew,et al.  Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21(DE3). , 2012, Journal of molecular biology.

[25]  Rachel Chen.  Bacterial expression systems for recombinant protein production: E. coli and beyond. , 2012, Biotechnology advances.

[26]  B. Byrne,et al.  Preparation of Pichia pastoris expression plasmids. , 2012, Methods in molecular biology.

[27]  R. Stroud,et al.  Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells , 2012, Nature Protocols.

[28]  R. Bill,et al.  Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review). , 2012, Methods in molecular biology.

[29]  D. Drew,et al.  Optimizing Saccharomyces cerevisiae induction regimes. , 2012, Methods in molecular biology.

[30]  D. Drew,et al.  Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins. , 2012, Methods in molecular biology.

[31]  D. Drew,et al.  Preparation of Saccharomyces cerevisiae expression plasmids. , 2012, Methods in molecular biology.

[32]  R. Stroud,et al.  Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells. , 2011, Methods.

[33]  J. de Gier,et al.  Consequences of the overexpression of a eukaryotic membrane protein, the human KDEL receptor, in Escherichia coli. , 2011, Journal of molecular biology.

[34]  Christopher G. Tate,et al.  Overcoming barriers to membrane protein structure determination , 2011, Nature Biotechnology.

[35]  S. Iwata,et al.  Benchmarking Membrane Protein Detergent Stability for Improving Throughput of High-Resolution X-ray Structures , 2011, Structure.

[36]  F. Kauff,et al.  Evolution of YidC/Oxa1/Alb3 insertases: three independent gene duplications followed by functional specialization in bacteria, mitochondria and chloroplasts , 2011, Biological chemistry.

[37]  B. Poolman,et al.  Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins. , 2010, Journal of molecular biology.

[38]  R. Stroud,et al.  Unlocking the eukaryotic membrane protein structural proteome. , 2010, Current opinion in structural biology.

[39]  B. Poolman,et al.  Amino Acid Accumulation Limits the Overexpression of Proteins in Lactococcus lactis , 2010, PloS one.

[40]  N. Rolland,et al.  Membrane protein expression in Lactococcus lactis. , 2010, Methods in molecular biology.

[41]  K. Brillet,et al.  Expression of membrane proteins in Drosophila Melanogaster S2 cells: Production and analysis of a EGFP-fused G protein-coupled receptor as a model. , 2010, Methods in molecular biology.

[42]  I. Mus-Veteau.  Heterologous expression of membrane proteins for structural analysis. , 2010, Methods in molecular biology.

[43]  A. Murphy,et al.  Functional expression and characterization of Arabidopsis ABCB, AUX 1 and PIN auxin transporters in Schizosaccharomyces pombe. , 2009, The Plant journal : for cell and molecular biology.

[44]  E. Gouaux,et al.  Crystal structure of the ATP-gated P2X4 ion channel in the closed state , 2009, Nature.

[45]  S. Palmer,et al.  Independent gene duplications of the YidC/Oxa/Alb3 family enabled a specialized cotranslational function , 2009, Proceedings of the National Academy of Sciences.

[46]  Zongli Hu,et al.  High human GLUT1, GLUT2, and GLUT3 expression in Schizosaccharomyces pombe , 2009, Biochemistry (Moscow).

[47]  K.,et al.  BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies. , 2008, Protein expression and purification.

[48]  R. Neutze,et al.  Effective high-throughput overproduction of membrane proteins in Escherichia coli. , 2008, Protein expression and purification.

[49]  Samuel Wagner,et al.  Tuning Escherichia coli for membrane protein overexpression , 2008, Proceedings of the National Academy of Sciences.

[50]  S. Thiem,et al.  A new Trichoplusia ni cell line for membrane protein expression using a baculovirus expression vector system , 2008, In Vitro Cellular & Developmental Biology - Animal.

[51]  Randall L. Kincaid,et al.  Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host , 2008, PloS one.

[52]  Jan Maarten van Dijl,et al.  Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes , 2008, Microbial cell factories.

[53]  G. Heijne,et al.  GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae , 2008, Nature Protocols.

[54]  K. V. van Wijk,et al.  Consequences of Membrane Protein Overexpression in Escherichia coli*S , 2007, Molecular & Cellular Proteomics.

[55]  G. von Heijne,et al.  High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae , 2007, Proceedings of the National Academy of Sciences.

[56]  A. Lüthi,et al.  Functional expression of mammalian receptors and membrane channels in different cells. , 2007, Journal of structural biology.

[57]  David J. Harvey,et al.  Glycoprotein Structural Genomics: Solving the Glycosylation Problem , 2007, Structure.

[58]  Jack Hoopes,et al.  Humanization of Yeast to Produce Complex Terminally Sialylated Glycoproteins , 2006, Science.

[59]  Samuel Wagner,et al.  Rationalizing membrane protein overexpression. , 2006, Trends in biotechnology.

[60]  D. Drew,et al.  Optimization of membrane protein overexpression and purification using GFP fusions , 2006, Nature Methods.

[61]  Eric Gouaux,et al.  Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. , 2006, Structure.

[62]  Carl W. Gunderson,et al.  Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system. , 2006, BioTechniques.

[63]  G. Friso,et al.  A scalable, GFP‐based pipeline for membrane protein overexpression screening and purification , 2005, Protein science : a publication of the Protein Society.

[64]  P. Loll.  Membrane protein structural biology: the high throughput challenge. , 2003, Journal of structural biology.

[65]  Dirk-Jan Slotboom,et al.  Lactococcus lactis as host for overproduction of functional membrane proteins. , 2003, Biochimica et biophysica acta.

[66]  G. von Heijne,et al.  Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli , 2001, FEBS letters.

[67]  J. Masson,et al.  Role of Sterols in Modulating the Human μ-Opioid Receptor Function in Saccharomyces cerevisiae * , 2000, The Journal of Biological Chemistry.

[68]  M. Runswick,et al.  Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over‐production of the b subunit of F1Fo ATP synthase , 2000, FEBS letters.

[69]  J. Walker,et al.  Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. , 1996, Journal of molecular biology.

[70]  P. A. Pedersen,et al.  Expression in High Yield of Pig α1β1 Na,K-ATPase and Inactive Mutants D369N and D807N in Saccharomyces cerevisiae(*) , 1996, The Journal of Biological Chemistry.

[71]  C. Scorer,et al.  Foreign gene expression in yeast: a review , 1992, Yeast.

[72]  M. Dreyfus,et al.  Bacteriophage T7 RNA polymerase travels far ahead of ribosomes in vivo , 1992, Journal of bacteriology.

[73]  J. Drews,et al.  Peptide chain initiation with chemically formylated Met-tRNAs from E. coli and yeast. , 1973, Biochemical and biophysical research communications.