Evaluation of sampling tools for environmental sampling of bacterial endospores from porous and nonporous surfaces

Aims:  Having and executing a well‐defined and validated sampling protocol is critical following a purposeful release of a biological agent for response and recovery activities, for clinical and epidemiological analysis and for forensic purposes. The objective of this study was to address the need for validated sampling and analysis methods called out by the General Accounting Office and others to systematically compare the collection efficiency of various swabs and wipes for collection of bacterial endospores from five different surfaces, both porous and nonporous. This study was also designed to test the collection and extraction solutions used for endospore recovery from swabs and wipes.

[1]  Linda D. Stetzenbach,et al.  Enhanced Detection of Surface-Associated Bacteria in Indoor Environments by Quantitative PCR , 2001, Applied and Environmental Microbiology.

[2]  Bruce Budowle,et al.  Toward a System of Microbial Forensics: from Sample Collection to Interpretation of Evidence , 2005, Applied and Environmental Microbiology.

[3]  J R Puleo,et al.  Wipe-rinse technique for quantitating microbial contamination on large surfaces , 1979, Applied and environmental microbiology.

[4]  L. J. Rose,et al.  Evaluation of a Macrofoam Swab Protocol for the Recovery of Bacillus anthracis Spores from a Steel Surface , 2006, Applied and Environmental Microbiology.

[5]  Misty J. Hein,et al.  Surface Sampling Methods for Bacillus anthracis Spore Contamination , 2002, Emerging infectious diseases.

[6]  Bruce Budowle,et al.  Building Microbial Forensics as a Response to Bioterrorism , 2003, Science.

[7]  R W Edwards,et al.  Microbiological sampling of surfaces. , 1968, The Journal of applied bacteriology.

[8]  John E. Brockmann,et al.  Evaluation of a Wipe Surface Sample Method for Collection of Bacillus Spores from Nonporous Surfaces , 2006, Applied and Environmental Microbiology.

[9]  Patricia Cruz,et al.  Evaluation of the Biological Sampling Kit (BiSKit) for Large-Area Surface Sampling , 2004, Applied and Environmental Microbiology.

[10]  Bruce Budowle,et al.  Quality Sample Collection, Handling, and Preservation for an Effective Microbial Forensics Program , 2006, Applied and Environmental Microbiology.

[11]  Paul Mead,et al.  Environmental Sampling for Spores of Bacillus anthracis , 2002, Emerging infectious diseases.

[12]  Bette Jensen,et al.  Swab Materials and Bacillus anthracis Spore Recovery from Nonporous Surfaces , 2004, Emerging infectious diseases.