The nucleotide sequence of apple mosaic virus coat protein gene has no similarity with other Bromoviridae coat protein genes.

A double-stranded cDNA was synthesized from in vitro polyadenylated apple mosaic virus (ApMV) RNA 3 using oligo(dT) and sequence-specific primers, and was cloned into plasmid vectors. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA 4. ApMV RNA 4 was found to contain an open reading frame (ORF) of 666 nucleotides, which was flanked by 5' and 3' non-translated sequences of 55 and 264 nucleotides, respectively. The ORF encoding the coat protein was identified by comparing the predicted amino acid sequence with that obtained from direct protein microsequencing of the native viral coat protein. The ORF encodes a protein with an M(r) of 25,056. The nucleotide sequence of the ApMV coat protein gene showed no similarity to those of alfalfa mosaic virus, tobacco streak virus (TSV), brome mosaic virus or cucumber mosaic virus. The predicted amino acid sequence of the amino-terminal region of the ApMV coat protein is basic, rich in cysteine residues and may contain a zinc finger motif similar to that found in TSV.

[1]  T. Hall,et al.  Complete nucleotide sequence of alfalfa mosaic virus RNA3. , 1983, Nucleic acids research.

[2]  R. Dildrop,et al.  Microsequence analysis of peptides and proteins , 1983 .

[3]  J. Johnson,et al.  A "zinc-finger"-type binding domain in tobacco streak virus coat protein. , 1989, Virology.

[4]  P. Ahlquist,et al.  Complete nucleotide sequence of brome mosaic virus RNA3. , 1981, Journal of molecular biology.

[5]  W. Fiers,et al.  Addition by ATP: RNA adenylyltransferase from Escherichia coli of 3'-linked oligo(A) to bacteriophage Qbeta RNA and its effect on RNA replication. , 1976, Biochimica et biophysica acta.

[6]  L. Vloten-Doting.  Coat protein is required for infectivity of tobacco streak virus: biological equivalence of the coat proteins of tobacco streak and alfalfa mosaic viruses. , 1975 .

[7]  B. Hoffman,et al.  A simple and very efficient method for generating cDNA libraries. , 1983, Gene.

[8]  V. Pallás,et al.  Nucleotide sequence of apple mosaic ilarvirus RNA 4. , 1994, The Journal of general virology.

[9]  J. Bol,et al.  Complete nucleotide sequence of alfalfa mosaic virus RNA 4. , 1980, Nucleic Acids Research.

[10]  Claude M. Fauquet,et al.  The classification and nomenclature of viruses , 1976, Archives of Virology.

[11]  L. Lane,et al.  Tricornaviridae - a proposed family of plant viruses with tripartite, single-stranded RNA genomes. , 1981, Intervirology.

[12]  E C Koper-Zwarthoff,et al.  Nucleotide sequence of the putative recognition site for coat protein in the RNAs of alfalfa mosaic virus and tobacco streak virus. , 1980, Nucleic acids research.

[13]  G. Veeneman,et al.  Complete nucleotide sequence of alfalfa mosaic virus RNA 2. , 1983, Nucleic acids research.

[14]  L. van Vloten-Doting.  Coat protein is required for infectivity of tobacco streak virus: biological equivalence of the coat proteins of tobacco streak and alfalfa mosaic viruses. , 1975, Virology.

[15]  G. Mink,et al.  Evaluation of agarose gel electrophoresis for resolving nucleoprotein components of Prunus necrotic ringspot virus , 1989 .

[16]  J. Bol,et al.  Complete nucleotide sequence of tobacco streak virus RNA 3. , 1984, Nucleic acids research.

[17]  L. Vloten-Doting,et al.  Plant Covirus Systems: Three-Component Systems , 1977 .

[18]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[19]  M. Fournier,et al.  cDNA library construction from small amounts of unfractionated RNA: association of cDNA synthesis with polymerase chain reaction amplification. , 1990, Analytical biochemistry.

[20]  J. Bol,et al.  Complete nucleotide sequence of alfalfa mosaic virus RNA 1. , 1983, Nucleic acids research.

[21]  Claude M. Fauquet,et al.  Classification and nomenclature of viruses. Fifth report of the International Committee on Taxonomy of Viruses. , 1991 .

[22]  F. Collins,et al.  Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. , 1991, Nucleic acids research.

[23]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[24]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[25]  R. Symons,et al.  Cucumber mosaic virus RNA 3. Determination of the nucleotide sequence provides the amino acid sequences of protein 3A and viral coat protein. , 2005, European journal of biochemistry.

[26]  S. L. Berger,et al.  First-strand cDNA synthesis primed with oligo(dT). , 1987, Methods in enzymology.

[27]  O. Sequeira.  Purification and serology of an Apple mosaic virus. , 1967 .