Microfluidic cell culture system studies and computational fluid dynamics

Microfluidic platforms are microfabricated tools that are gaining popularity for studying of cellular biology. These platforms can allow precise control of the environment surrounding individual cells and have achieved amazing progress in application to cell culture in recent several years. After a brief description of the background and the major characteristics of the microfluidic chip and the corresponding advantages for cell culture, the development of cell patterning techniques is discussed. The microfluidic cell culture platforms are classified into: (i) single cell-type culture; and, (ii) multiple cell-type culture (i.e., cell coculture). Several microfluidic cell culture systems integrated with other functionalities using microfabricated technology are reviewed. This review also aims at discussing the rationale of using computational fluid dynamics (CFD) in microfluidic cell culture system studies, as fluid flow processes and phenomena have direct implications on cellular response such as migration and/or proliferation.

[1]  Mats Nilsson,et al.  Thermoplastic microfluidic platform for single-molecule detection, cell culture, and actuation. , 2005, Analytical chemistry.

[2]  J. D. Felske,et al.  Microfluidic chip to produce temperature jumps for electrophysiology. , 2008, Analytical chemistry.

[3]  M. Schenkel,et al.  Charles University in Prague Faculty of Mathematics and Physics , 2013 .

[4]  Investigating the Effects of Fluid Shear Forces on Cellular Responses to Profiled Surfaces in-Vitro: A Computational and Experimental Investigation , 2007, 2007 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[5]  A. Scherer,et al.  Applications of microfluidics for neuronal studies , 2007, Journal of the Neurological Sciences.

[6]  Man Bock Gu,et al.  Fabrication of a bio-MEMS based cell-chip for toxicity monitoring. , 2007, Biosensors & bioelectronics.

[7]  D. Beebe,et al.  Cell culture models in microfluidic systems. , 2008, Annual review of analytical chemistry.

[8]  Ali Khademhosseini,et al.  A simple soft lithographic route to fabrication of poly(ethylene glycol) microstructures for protein and cell patterning. , 2004, Biomaterials.

[9]  H. Kimura,et al.  An integrated microfluidic system for long-term perfusion culture and on-line monitoring of intestinal tissue models. , 2008, Lab on a chip.

[10]  Andrew J deMello,et al.  Micro- and nanofluidic systems for high-throughput biological screening. , 2009, Drug discovery today.

[11]  S. Quake,et al.  Versatile, fully automated, microfluidic cell culture system. , 2007, Analytical chemistry.

[12]  D. Beebe,et al.  Microenvironment design considerations for cellular scale studies. , 2004, Lab on a chip.

[13]  Hanry Yu,et al.  A novel 3D mammalian cell perfusion-culture system in microfluidic channels. , 2007, Lab on a chip.

[14]  Cheng-Hsien Liu,et al.  Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap. , 2006, Lab on a chip.

[15]  David J. Odde,et al.  Micro-Patterning of Animal Cells on PDMS Substrates in the Presence of Serum without Use of Adhesion Inhibitors , 2004, Biomedical microdevices.

[16]  Luke P. Lee,et al.  A novel high aspect ratio microfluidic design to provide a stable and uniform microenvironment for cell growth in a high throughput mammalian cell culture array. , 2005, Lab on a chip.

[17]  K. Jensen,et al.  Cells on chips , 2006, Nature.

[18]  Cheng-Wey Wei,et al.  Elucidating in vitro cell-cell interaction using a microfluidic coculture system , 2006, Biomedical microdevices.

[19]  David J. Beebe,et al.  Insect Cell Culture in Microfluidic Channels , 2002 .

[20]  L. Griffith,et al.  A microfabricated array bioreactor for perfused 3D liver culture. , 2002, Biotechnology and bioengineering.

[21]  Vernella Vickerman,et al.  Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imaging. , 2008, Lab on a chip.

[22]  D. Beebe,et al.  Microfluidic technology for assisted reproduction. , 2002, Theriogenology.

[23]  Laurent Griscom,et al.  In situ micropatterning technique by cell crushing for co-cultures inside microfluidic biochips , 2008, Biomedical microdevices.

[24]  D W Hutmacher,et al.  Bioreactor studies and computational fluid dynamics. , 2009, Advances in biochemical engineering/biotechnology.

[25]  Hanry Yu,et al.  A practical guide to microfluidic perfusion culture of adherent mammalian cells. , 2007, Lab on a chip.

[26]  Mengsu Yang,et al.  Microfluidics technology for manipulation and analysis of biological cells , 2006 .

[27]  Dmytryshyn.  Microfluidic cell culture systems and cellular analysis , 2011 .

[28]  Harmeet Singh,et al.  Computational fluid dynamics for improved bioreactor design and 3D culture. , 2008, Trends in biotechnology.

[29]  B Lepioufle,et al.  Study of osteoblastic cells in a microfluidic environment. , 2006, Biomaterials.

[30]  Luke P. Lee,et al.  Integrated microfluidic cell culture and lysis on a chip. , 2007, Lab on a chip.

[31]  Kahp Yang Suh,et al.  Cell research with physically modified microfluidic channels: a review. , 2008, Lab on a chip.

[32]  Gwo-Bin Lee,et al.  A Microfluidic System for Automatic Cell Culture , 2007 .

[33]  Dieter Trau,et al.  In-situ measurement of cellular microenvironments in a microfluidic device. , 2009, Lab on a chip.

[34]  Luke P. Lee,et al.  Nanoliter scale microbioreactor array for quantitative cell biology , 2006, Biotechnology and bioengineering.

[35]  Y Wakamoto,et al.  On-chip culture system for observation of isolated individual cells. , 2001, Lab on a chip.

[36]  Lung-Jieh Yang,et al.  The micro patterning of glutaraldehyde (GA)-crosslinked gelatin and its application to cell-culture. , 2005, Lab on a chip.

[37]  Xingyu Jiang,et al.  A method for patterning multiple types of cells by using electrochemical desorption of self-assembled monolayers within microfluidic channels. , 2007, Angewandte Chemie.

[38]  T. Park,et al.  Integration of Cell Culture and Microfabrication Technology , 2003, Biotechnology progress.

[39]  Luke P. Lee,et al.  Continuous perfusion microfluidic cell culture array for high-throughput cell-based assays. , 2005, Biotechnology and bioengineering.

[40]  D W Hutmacher,et al.  Flow modelling within a scaffold under the influence of uni-axial and bi-axial bioreactor rotation. , 2005, Journal of biotechnology.

[41]  Hsian-Rong Tseng,et al.  An integrated microfluidic culture device for quantitative analysis of human embryonic stem cells. , 2009, Lab on a chip.