Annals of Clinical Microbiology and Antimicrobials Low-cost Rapid Detection of Rifampicin Resistant Tuberculosis Using Bacteriophage in Kampala, Uganda

Background: Resistance to anti-tuberculosis drugs is a serious public health problem. Multi-drug resistant tuberculosis (MDR-TB), defined as resistance to at least rifampicin and isoniazid, has been reported in all regions of the world. Current phenotypic methods of assessing drug susceptibility of M. tuberculosis are slow. Rapid molecular methods to detect resistance to rifampicin have been developed but they are not affordable in some high prevalence countries such as those in sub Saharan Africa. A simple multi-well plate assay using mycobacteriophage D29 has been developed to test M. tuberculosis isolates for resistance to rifampicin. The purpose of this study was to investigate the performance of this technology in Kampala, Uganda.

[1]  J. Palomino,et al.  Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in Mycobacterium tuberculosis , 2006, Annals of Clinical Microbiology and Antimicrobials.

[2]  Madhukar Pai,et al.  A commercial line probe assay for the rapid detection of rifampicin resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis , 2005, BMC infectious diseases.

[3]  J. Palomino,et al.  In-House Phage Amplification Assay Is a Sound Alternative for Detecting Rifampin-Resistant Mycobacterium tuberculosis in Low-Resource Settings , 2005, Antimicrobial Agents and Chemotherapy.

[4]  S. Narayanan,et al.  Comparison of DNA sequencing, PCR-SSCP and PhaB assays with indirect sensitivity testing for detection of rifampicin resistance in Mycobacterium tuberculosis. , 2003, The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease.

[5]  C. Prat,et al.  Utility of an In-House Mycobacteriophage-Based Assay for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Clinical Isolates , 2003, Journal of Clinical Microbiology.

[6]  L. Blumberg,et al.  Rapid indication of multidrug-resistant tuberculosis from liquid cultures using FASTPlaqueTB-RIF, a manual phage-based test. , 2002, The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease.

[7]  S. Niemann,et al.  Frequency of rpoB Mutations Inside and Outside the Cluster I Region in Rifampin-Resistant Clinical Mycobacterium tuberculosis Isolates , 2001, Journal of Clinical Microbiology.

[8]  I. Bastian,et al.  Detection of rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as an initial indicator of multidrug resistance. , 2000, The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease.

[9]  H. Miörner,et al.  Evaluation of a colorimetric assay based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) for rapid detection of rifampicin resistance in Mycobacterium tuberculosis. , 1998, The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease.

[10]  K. Yanagihara,et al.  Rapid prediction of rifampin susceptibility of Mycobacterium tuberculosis. , 1997, American journal of respiratory and critical care medicine.

[11]  F. Drobniewski,et al.  Evaluation of a new rapid bacteriophage-based method for the drug susceptibility testing of Mycobacterium tuberculosis , 1997, Nature Medicine.

[12]  A. Telenti,et al.  Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis , 1993, The Lancet.

[13]  J. T. Crawford,et al.  Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology , 1993, Journal of clinical microbiology.

[14]  R. McNerney,et al.  Micro-well phage replication assay for screening mycobacteria for resistance to rifampin and streptomycin. , 2001, Methods in molecular medicine.

[15]  P. Kiepiela,et al.  Rapid screening of Mycobacterium tuberculosis for susceptibility to rifampicin and streptomycin. , 2000, The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease.