Overrepresentation of a Gene Family Encoding Extracytoplasmic Solute Receptors in Bordetella

ABSTRACT A family of genes that are likely to encode extracytoplasmic solute receptors is strongly overrepresented in several β-proteobacteria, including Bordetella pertussis. This gene family, of which members have been called bug genes, contains some examples that are contained within polycistronic operons coding for tripartite uptake transporters of the TTT family, while the vast majority are “orphan” genes. Proteomic and functional analyses demonstrated that several of these genes are expressed in B. pertussis, and one is involved in citrate uptake. The bug genes probably form an ancient family that has been subjected to a large expansion in a restricted phylogenic group.

[1]  R. Gross,et al.  Identification and genomic organization of gene loci negatively controlled by the virulence regulatory BvgAS two-component system in Bordetella bronchiseptica , 2002, Molecular Genetics and Genomics.

[2]  M. Simmonds,et al.  Genome sequence of Yersinia pestis, the causative agent of plague , 2001, Nature.

[3]  C. Locht,et al.  Role of ADP-Ribosyltransferase Activity of Pertussis Toxin in Toxin-Adhesin Redundancy with Filamentous Hemagglutinin duringBordetella pertussis Infection , 2001, Infection and Immunity.

[4]  C. Locht,et al.  Subtilisin‐like autotransporter serves as maturation protease in a bacterial secretion pathway , 2001, The EMBO journal.

[5]  U. Göbel,et al.  Bordetella petrii sp. nov., isolated from an anaerobic bioreactor, and emended description of the genus Bordetella. , 2001, International journal of systematic and evolutionary microbiology.

[6]  C. Guzmán,et al.  The virulence factors of Bordetella pertussis: a matter of control. , 2001, FEMS microbiology reviews.

[7]  B. Barrell,et al.  Massive gene decay in the leprosy bacillus , 2001, Nature.

[8]  C. Locht,et al.  Bordetella pertussis, molecular pathogenesis under multiple aspects. , 2001, Current opinion in microbiology.

[9]  S. Falkow,et al.  mig-14 Is a Horizontally Acquired, Host-Induced Gene Required for Salmonella enterica Lethal Infection in the Murine Model of Typhoid Fever , 2000, Infection and Immunity.

[10]  E. Willery,et al.  New Virulence-Activated and Virulence-Repressed Genes Identified by Systematic Gene Inactivation and Generation of Transcriptional Fusions in Bordetella pertussis , 2000, Journal of bacteriology.

[11]  C. Locht,et al.  Genomics of Bordetella pertussis toxins. , 2000, International journal of medical microbiology : IJMM.

[12]  I. Paulsen,et al.  Microbial genome analyses: comparative transport capabilities in eighteen prokaryotes. , 2000, Journal of molecular biology.

[13]  I. Paulsen,et al.  Paralogous genes encoding transport proteins in microbial genomes. , 1999, Research in microbiology.

[14]  C. Hoogland,et al.  '98 Escherichia coli SWISS‐2DPAGE database update , 1998, Electrophoresis.

[15]  B. Barrell,et al.  Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence , 1998, Nature.

[16]  A. Goffeau,et al.  The complete genome sequence of the Gram-positive bacterium Bacillus subtilis , 1997, Nature.

[17]  D. Kelly,et al.  TRAP transporters: a new family of periplasmic solute transport systems encoded by the dctPQM genes of Rhodobacter capsulatus and by homologs in diverse gram-negative bacteria , 1997, Journal of bacteriology.

[18]  J. H. Hannah,et al.  Heparin-inhibitable lectin activity of the filamentous hemagglutinin adhesin of Bordetella pertussis , 1994, Infection and immunity.

[19]  M H Saier,et al.  Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria , 1993, Microbiological reviews.

[20]  J. Mekalanos,et al.  Repressor binding to a regulatory site in the DNA coding sequence is sufficient to confer transcriptional regulation of the vir-repressed genes (vrg genes) in Bordetella pertussis , 1993, Journal of bacteriology.

[21]  C. Locht,et al.  Common accessory genes for the Bordetella pertussis filamentous hemagglutinin and fimbriae share sequence similarities with the papC and papD gene families. , 1992, The EMBO journal.

[22]  S. Knapp,et al.  Evidence that modulation requires sequences downstream of the promoters of two vir-repressed genes of Bordetella pertussis , 1990, Journal of bacteriology.

[23]  W. Kay,et al.  Genetic regulation of the tricarboxylate transport operon (tctI) of Salmonella typhimurium , 1989, Journal of bacteriology.

[24]  W. Kay,et al.  Expression of the divergent tricarboxylate transport operon (tctI) of Salmonella typhimurium , 1988, Journal of bacteriology.

[25]  W. Boos,et al.  Cloning and properties of the Salmonella typhimurium tricarboxylate transport operon in Escherichia coli , 1988, Journal of bacteriology.

[26]  C. Kay,et al.  Tricarboxylate-binding proteins of Salmonella typhimurium. Purification, crystallization, and physical properties. , 1984, The Journal of biological chemistry.

[27]  F. von Wintzingerode,et al.  Evolutionary trends in the genus Bordetella. , 2001, Microbes and infection.

[28]  V. DiRita,et al.  Bacterial virulence gene regulation: an evolutionary perspective. , 2000, Annual review of microbiology.

[29]  C. Kay,et al.  Tricarboxylate-binding Proteins of Salmonella typhimurium , 1984 .