Based on the elastic foundation model and wave theory as well as fluid mechanics theory, considered of the influence of flow velocity in the pipe, vertical force of buried water supply pipeline are analyzed under the action of seismic wave. The bending moment and stress formula are derived, and the vertical stress calculation model of water supply pipeline under the action of seismic wave was established. The influence of different diameter, flow velocity, soil and earthquake intensity on the water supply pipeline is analyzed by numerical examples. Keywords-water supply pipeline; seismic wave; stress; calculation model; flow velocity