Potential Linkages Between the Inner and Outer Cellular States of Human Induced Pluripotent Stem Cells

We analyzed both RNA profile to reveal gene expression changes and glycan profile to identify structural changes in glycans between four parental somatic cell (SC) lines and nine hiPSC lines that were originally established. The sum of these information by a combination of standard statistical techniques and a network approach showed significant differences in expression between the iPSCs and SCs. Subsequent network analysis of the gene expression and glycan signatures revealed glycan transfer network associated with known epitopes for differentiation. The present study is the first to uncover the relationships between gene expression patterns and cell surface changes in hiPSCs, and reinforces the importance of the cell surface to identify established iPSCs from SCs. The Fourth International Conference on Computational Systems Biology (ISB2010) Suzhou, China, September 9–11, 2010 Copyright © 2010 ORSC & APORC, pp. 381–388

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