Spatial comparison between wall shear stress measures and porcine arterial endothelial permeability.

A better understanding of how hemodynamic factors affect the integrity and function of the vascular endothelium is necessary to appreciate more fully how atherosclerosis is initiated and promoted. A novel technique is presented to assess the relation between fluid dynamic variables and the permeability of the endothelium to macromolecules. Fully anesthetized, domestic swine were intravenously injected with the albumin marker Evans blue dye, which was allowed to circulate for 90 min. After the animals were euthanized, silicone casts were made of the abdominal aorta and its iliac branches. Pulsatile flow calculations were subsequently made in computational regions derived from the casts. The distribution of the calculated time-dependent wall shear stress in the external iliac branches was directly compared on a point-by-point basis with the spatially varying in vivo uptake of Evans blue dye in the same arteries. The results indicate that in vivo endothelial permeability to albumin decreases with increasing time-average shear stress over the normal range. Additionally, endothelial permeability increases slightly with oscillatory shear index.

[1]  C. J. Schwartz,et al.  Focal 3 H-cholesterol uptake in the pig aorta. , 1971, Atherosclerosis.

[2]  H. Luginbühl,et al.  The domestic pig: a model for experimental atherosclerosis. , 1971, Atherosclerosis.

[3]  R. Schroter,et al.  Atheroma and arterial wall shear - Observation, correlation and proposal of a shear dependent mass transfer mechanism for atherogenesis , 1971, Proceedings of the Royal Society of London. Series B. Biological Sciences.

[4]  D. J. Patel,et al.  Effect of tensile and shear stress on intimal permeability of the left coronary artery in dogs. , 1973, Atherosclerosis.

[5]  C. Caro.  Transport of 14C-4-cholesterol between perfusing serum and dog common carotid artery: a shear dependent process. , 1974, Cardiovascular research.

[6]  R. Nerem,et al.  Transendothelial transport of 131I-albumin. , 1976, Biorheology.

[7]  Simultaneous accumulation of Evans blue dye and albumin in the canine aortic wall. , 1977, The American journal of physiology.

[8]  T. M. Hollis,et al.  Influence of locally altered in vivo shear stress on aortic histamine-forming capacity and aortic albumin uptake. , 1981, Blood vessels.

[9]  G. Hutchins,et al.  Arterial geometry affects hemodynamics. A potential risk factor for athersoclerosis. , 1983, Atherosclerosis.

[10]  D. Ku,et al.  Pulsatile Flow and Atherosclerosis in the Human Carotid Bifurcation: Positive Correlation between Plaque Location and Low and Oscillating Shear Stress , 1985, Arteriosclerosis.

[11]  M. Debakey,et al.  Patterns of Atherosclerosis and their Surgical Significance , 1985, Annals of surgery.

[12]  M. H. Friedman,et al.  Variations in geometry and shear rate distribution in casts of human aortic bifurcations. , 1989, Journal of biomechanics.

[13]  D D Duncan,et al.  The effect of compliance on wall shear in casts of a human aortic bifurcation. , 1990, Journal of biomechanical engineering.

[14]  J. Tarbell,et al.  Endothelial albumin permeability is shear dependent, time dependent, and reversible. , 1991, The American journal of physiology.

[15]  B. Nordestgaard,et al.  Aortic permeability to LDL as a predictor of aortic cholesterol accumulation in cholesterol-fed rabbits. , 1992, Arteriosclerosis and thrombosis : a journal of vascular biology.

[16]  C. M. Waters,et al.  Flow‐induced modulation of the permeability of endothelial cells cultured on microcarrier beads , 1996, Journal of cellular physiology.

[17]  D. Ku,et al.  Pulsatile flow in the human left coronary artery bifurcation: average conditions. , 1996, Journal of biomechanical engineering.

[18]  D Kaiser,et al.  Lack of hemodynamic forces triggers apoptosis in vascular endothelial cells. , 1997, Biochemical and biophysical research communications.

[19]  B. Rutt,et al.  Hemodynamics of human carotid artery bifurcations: computational studies with models reconstructed from magnetic resonance imaging of normal subjects. , 1998, Journal of vascular surgery.

[20]  M H Friedman,et al.  Variability of the planarity of the human aortic bifurcation. , 1998, Medical engineering & physics.

[21]  C Kleinstreuer,et al.  Relation between non-uniform hemodynamics and sites of altered permeability and lesion growth at the rabbit aorto-celiac junction. , 1999, Atherosclerosis.

[22]  J. Henderson,et al.  Effect of alterations in femoral artery flow on abdominal vessel hemodynamics in swine. , 1999, Biorheology.

[23]  T. David,et al.  Computational Models of Blood Flow in the Circle of Willis , 2001, Computer methods in biomechanics and biomedical engineering.

[24]  J. Henderson,et al.  Effect of periodic alterations in shear on vascular macromolecular uptake. , 2000, Biorheology.

[25]  B. McManus,et al.  Endothelial cell apoptosis: biochemical characteristics and potential implications for atherosclerosis. , 2001, Journal of molecular and cellular cardiology.

[26]  P. Lin,et al.  Shear stress regulates occludin and VEGF expression in porcine arterial endothelial cells. , 2002, The Journal of surgical research.

[27]  K Affeld,et al.  Atherosclerosis and flow in carotid arteries with authentic geometries. , 2002, Biorheology.

[28]  M. H. Friedman.  Variability of 3D arterial geometry and dynamics, and its pathologic implications. , 2002, Biorheology.

[29]  C. P. Winlove,et al.  Effects of Perfusion Rate on Permeability of Frog and Rat Mesenteric Microvessels to Sodium Fluorescein , 2002, The Journal of physiology.

[30]  G. Truskey,et al.  Effect of Fluid Shear Stress on the Permeability of the Arterial Endothelium , 2002, Annals of Biomedical Engineering.

[31]  A. Hazel,et al.  Method for Assessing the Need for Case-Specific Hemodynamics: Application to the Distribution of Vascular Permeability , 2000, Annals of Biomedical Engineering.