Vertebrate hepatic lipase genes and proteins: a review supported by bioinformatic studies.

Hepatic lipase (gene: LIPC; enzyme: HL; E.C.3.1.1.3) is one of three members of the triglyceride lipase family that contributes to vascular lipoprotein degradation and serves a dual role in triglyceride hydrolysis and in facilitating receptor-mediated lipoprotein uptake into the liver. Amino acid sequences, protein structures, and gene locations for vertebrate LIPC (or Lipc for mouse and rat) genes and proteins were sourced from previous reports and vertebrate genome databases. Lipc was distinct from other neutral lipase genes (Lipg encoding endothelial lipase and Lpl encoding lipoprotein lipase [LPL]) and was located on mouse chromosome 9 with nine coding exons on the negative strand. Exon 9 of human LIPC and mouse and rat Lipc genes contained "stop codons" in different positions, causing changes in C-termini length. Vertebrate HL protein subunits shared 58%-97% sequence identities, including active, signal peptide, disulfide bond, and N-glycosylation sites, as well as proprotein convertase ("hinge") and heparin binding regions. Predicted secondary and tertiary structures revealed similarities with the three-dimensional structure reported for horse and human pancreatic lipases. Potential sites for regulating LIPC gene expression included CpG islands near the 5″-untranslated regions of the mouse and rat LIPC genes. Phylogenetic analyses examined the relationships and potential evolutionary origins of the vertebrate LIPC gene family with other neutral triglyceride lipase gene families (LIPG and LPL). We conclude that the triglyceride lipase ancestral gene for vertebrate neutral lipase genes (LIPC, LIPG, and LPL) predated the appearance of fish during vertebrate evolution.

[1]  J. VandeBerg,et al.  Vertebrate endothelial lipase: comparative studies of an ancient gene and protein in vertebrate evolution , 2011, Genetica.

[2]  E. Rimm,et al.  Association between hepatic lipase -514 C/T promoter polymorphism and myocardial infarction is modified by history of hypercholesterolemia and waist circumference. , 2010, Nutrition, metabolism, and cardiovascular diseases : NMCD.

[3]  Li Meng,et al.  Association of LIPC -250G>A polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations , 2010, Lipids in Health and Disease.

[4]  David W. Williamson,et al.  Polymorphisms in the hepatic lipase gene affect plasma HDL-cholesterol levels in a Turkish population[S] , 2010, Journal of Lipid Research.

[5]  P. Ridker,et al.  Forty-Three Loci Associated with Plasma Lipoprotein Size, Concentration, and Cholesterol Content in Genome-Wide Analysis , 2009, PLoS genetics.

[6]  Thomas J Petty,et al.  Identification of the Active Form of Endothelial Lipase, a Homodimer in a Head-to-Tail Conformation* , 2009, The Journal of Biological Chemistry.

[7]  D. Rader,et al.  Lipases as modulators of atherosclerosis in murine models. , 2007, Current drug targets.

[8]  M. Benton,et al.  Rocks and clocks: calibrating the Tree of Life using fossils and molecules. , 2007, Trends in ecology & evolution.

[9]  Bronwen L. Aken,et al.  Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences , 2007, Nature.

[10]  S. Brunak,et al.  Locating proteins in the cell using TargetP, SignalP and related tools , 2007, Nature Protocols.

[11]  J. Thierry-Mieg,et al.  AceView: a comprehensive cDNA-supported gene and transcripts annotation , 2006, Genome Biology.

[12]  B. Foeger,et al.  Hepatic lipase polymorphism and increased risk of peripheral arterial disease , 2005, Journal of internal medicine.

[13]  Jean L. Chang,et al.  Initial sequence of the chimpanzee genome and comparison with the human genome , 2005, Nature.

[14]  Colin N. Dewey,et al.  Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution , 2004, Nature.

[15]  D. Rader,et al.  Structural basis of endothelial lipase tropism for HDL , 2004, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[16]  H. Brewer,et al.  The Ligand-binding Function of Hepatic Lipase Modulates the Development of Atherosclerosis in Transgenic Mice* , 2004, Journal of Biological Chemistry.

[17]  M. Hutz,et al.  Association between −250G/A polymorphism of the hepatic lipase gene promoter and coronary artery disease and HDL‐C levels in a Southern Brazilian population , 2004, Clinical genetics.

[18]  Lisa M. D'Souza,et al.  Genome sequence of the Brown Norway rat yields insights into mammalian evolution , 2004, Nature.

[19]  Somasekar Seshagiri,et al.  The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. , 2003, Genome research.

[20]  C. Ballantyne,et al.  Endothelial lipase is a major genetic determinant for high-density lipoprotein concentration, structure, and metabolism , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[21]  Jonathan C. Cohen.  Endothelial lipase: direct evidence for a role in HDL metabolism. , 2003, The Journal of clinical investigation.

[22]  Colin N. Dewey,et al.  Initial sequencing and comparative analysis of the mouse genome. , 2002 .

[23]  L. Martinez,et al.  Hepatic lipase: structure/function relationship, synthesis, and regulation. , 2002, Journal of lipid research.

[24]  Tom H. Pringle,et al.  The human genome browser at UCSC. , 2002, Genome research.

[25]  J. V. Moran,et al.  Initial sequencing and analysis of the human genome. , 2001, Nature.

[26]  Liam J. McGuffin,et al.  The PSIPRED protein structure prediction server , 2000, Bioinform..

[27]  R. Sendak,et al.  Binding of hepatic lipase to heparin. Identification of specific heparin-binding residues in two distinct positive charge clusters. , 2000, Journal of lipid research.

[28]  Sungshin Y. Choi,et al.  Cloning of a Unique Lipase from Endothelial Cells Extends the Lipase Gene Family* , 1999, The Journal of Biological Chemistry.

[29]  C. Haudenschild,et al.  The role of hepatic lipase in lipoprotein metabolism and atherosclerosis. , 1999, Current opinion in lipidology.

[30]  D. Rader,et al.  A novel endothelial-derived lipase that modulates HDL metabolism , 1999, Nature Genetics.

[31]  VivianeNicaud,et al.  Hepatic Lipase Gene Polymorphisms Influence Plasma HDL Levels , 1997 .

[32]  J. Taylor,et al.  Hepatic lipase is abundant on both hepatocyte and endothelial cell surfaces in the liver. , 1997, Journal of lipid research.

[33]  S. Grundy,et al.  A hepatic lipase (LIPC) allele associated with high plasma concentrations of high density lipoprotein cholesterol. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[34]  R. Willemsen,et al.  Hepatic lipase is localized at the parenchymal cell microvilli in rat liver. , 1997, The Biochemical journal.

[35]  N. Maeda,et al.  Analysis of Protein Structure-Function in Vivo , 1996, The Journal of Biological Chemistry.

[36]  M. Hayden,et al.  Premature atherosclerosis in patients with familial chylomicronemia caused by mutations in the lipoprotein lipase gene. , 1996, The New England journal of medicine.

[37]  J. Philo,et al.  Human Hepatic Lipase Subunit Structure Determination* , 1996, The Journal of Biological Chemistry.

[38]  K. Dugi,et al.  Human Hepatic and Lipoprotein Lipase: The Loop Covering the Catalytic Site Mediates Lipase Substrate Specificity (*) , 1995, The Journal of Biological Chemistry.

[39]  H. van Tilbeurgh,et al.  The 2.46 A resolution structure of the pancreatic lipase-colipase complex inhibited by a C11 alkyl phosphonate. , 1994, Biochemistry.

[40]  G. Liu,et al.  Lipoprotein lipase and hepatic lipase: the role of asparagine-linked glycosylation in the expression of a functional enzyme. , 1994, Journal of lipid research.

[41]  Yves Van de Peer,et al.  TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment , 1994, Comput. Appl. Biosci..

[42]  C Cambillau,et al.  Horse pancreatic lipase. The crystal structure refined at 2.3 A resolution. , 1994, Journal of molecular biology.

[43]  J. Wölle,et al.  Functional role of N-linked glycosylation in human hepatic lipase: asparagine-56 is important for both enzyme activity and secretion. , 1993, Journal of lipid research.

[44]  J. Hokanson,et al.  Effect of hepatic lipase on LDL in normal men and those with coronary artery disease. , 1993, Arteriosclerosis and thrombosis : a journal of vascular biology.

[45]  R. Davis,et al.  Domain exchange: characterization of a chimeric lipase of hepatic lipase and lipoprotein lipase. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[46]  E. Boerwinkle,et al.  Role of apolipoprotein E and B gene variation in determining response of lipid, lipoprotein, and apolipoprotein levels to increased dietary cholesterol. , 1991, American journal of human genetics.

[47]  H. Netter,et al.  Characterization of cDNA encoding the mouse hepatic triglyceride lipase and expression by in vitro translation , 1991, FEBS letters.

[48]  H. Brewer,et al.  Identification of two separate allelic mutations in the lipoprotein lipase gene of a patient with the familial hyperchylomicronemia syndrome. , 1991, The Journal of biological chemistry.

[49]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[50]  J. Kobayashi,et al.  Isolation and characterization of the human hepatic lipase gene. , 1990, The Journal of biological chemistry.

[51]  M. Sensel,et al.  Isolation and characterization of clones for the rat hepatic lipase gene upstream regulatory region. , 1990, Biochimica et biophysica acta.

[52]  F. Winkler,et al.  Structure of human pancreatic lipase , 1990, Nature.

[53]  L. Chan,et al.  Structure of the human hepatic triglyceride lipase gene. , 1989, Biochemistry.

[54]  M. Schafer,et al.  Isolation and cDNA sequence of human postheparin plasma hepatic triglyceride lipase. , 1988, The Journal of biological chemistry.

[55]  D. Ledbetter,et al.  Human hepatic lipase. Cloned cDNA sequence, restriction fragment length polymorphisms, chromosomal localization, and evolutionary relationships with lipoprotein lipase and pancreatic lipase. , 1988, The Journal of biological chemistry.

[56]  R. Sprengel,et al.  Human hepatic triglyceride lipase: cDNA cloning, amino acid sequence and expression in a cultured cell line. , 1987, Differentiation; research in biological diversity.

[57]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[58]  T. Kirchgessner,et al.  Human lipoprotein lipase complementary DNA sequence. , 1987, Science.

[59]  A. Bensadoun,et al.  Characterization of the intracellular processing and secretion of hepatic lipase in FU5AH rat hepatoma cells. , 1987, Biochimica et biophysica acta.

[60]  M. Komaromy,et al.  Cloning of rat hepatic lipase cDNA: evidence for a lipase gene family. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[61]  T. Olivecrona,et al.  Mouse preheparin plasma contains high levels of hepatic lipase with low affinity for heparin. , 1986, Biochimica et biophysica acta.

[62]  J. Felsenstein.  CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP , 1985, Evolution; international journal of organic evolution.

[63]  K. Holsinger.  The Neutral Theory of Molecular Evolution. , 2012 .

[64]  N. Seidah,et al.  Proprotein convertases [corrected] are responsible for proteolysis and inactivation of endothelial lipase. , 2005, The Journal of biological chemistry.

[65]  Ryan D. Morin,et al.  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). , 2004, Genome research.

[66]  Torsten Schwede,et al.  The SWISS-MODEL Repository of annotated three-dimensional protein structure homology models , 2004, Nucleic Acids Res..

[67]  T. A. Hall,et al.  BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT , 1999 .

[68]  R. Hegele,et al.  Human hepatic lipase mutations and polymorphisms , 1992, Human mutation.

[69]  M. Nei,et al.  The neighbor-joining method , 1987 .