Fine‐tuning the prediction of sequences cleaved by signal peptidase II: A curated set of proven and predicted lipoproteins of Escherichia coli K‐12

A curated set of 81 proven and 44 predicted lipoproteins of Escherichia coli K‐12 was defined with the combined use of a literature survey, a variety of predictive tools and human expertise. The well‐documented Gram‐negative proteome of E. coli K‐12 was chosen to assess how the different approaches complement each other and to ensure a stable definition of a consistent set of lipoproteins. The results of detailed analysis of such proteins at the level of a single proteome are presented, corroborated and rationalized.