The aim of this study was to evaluate the amount of dust in supply air ducts in recently installed ventilation systems. The samples for the determination of dust accumulation were collected from supply air ducts in 18 new buildings that have been constructed according to two different cleanliness control levels classified as category P1 (low oil residues and protected against contaminations) and category P2, as defined in the Classification of Indoor Climate, Construction and Building Materials. In the ducts installed according to the requirements of cleanliness category P1 the mean amount of accumulated dust was 0.9 g/m2 (0.4-2.9 g/m2), and in the ducts installed according to the cleanliness category P2 it was 2.3 g/m2 (1.2-4.9 g/m2). A significant difference was found in the mean amounts of dust between ducts of categories P1 and P2 (P < 0.008). The cleanliness control procedure in category P1 proved to be a useful and effective tool for preventing dust accumulation in new air ducts during the construction process. Additionally, the ducts without residual oil had lower amounts of accumulated dust indicating that the demand for oil free components in the cleanliness classification is reasonable.