Cloning and expression analysis of KfNCED1 from Kalidium foliatum (Chenopodiaceae) in response to NaCl treatment

Kalidium foliatum exhibits strong tolerance to salinity. NCED 1 from K. foliatum was cloned, and its expression level was analysed under salt treatment. Whole plants were subjected to 0, 150, 200, 250, 300, 350 and 400 mM NaCl for 72 h. Total RNA was extracted from the leaves of 4-month-old K. foliatum seedlings. The full-length KfNCED 1 cDNA was cloned with reverse transcription PCR, the KfNCED 1 expression levels were analysed with semiquantitative RT-PCR, and the ABA contents from K. foliatum were examined. The KfNCED 1 cDNA was 1500 bp long and included an open reading frame that encoded a polypeptide of 499 amino acids. The deduced amino acid sequence of KfNCED 1 had significant similarity with that of AiNCED1 . Semiquantitative RT-PCR showed that the expression of KfNCED 1 could be markedly induced by salt treatment.

[1]  P. Ahmad,et al.  Physiological, biochemical, and antioxidant properties of two genotypes of Vicia faba grown under salinity stress , 2019, Pakistan Journal of Botany.

[2]  Peipei Xu,et al.  Functional characterization of the BnNCED3 gene in Brassica napus. , 2017, Plant science : an international journal of experimental plant biology.

[3]  M. S. Hossain,et al.  Tuning of Redox Regulatory Mechanisms, Reactive Oxygen Species and Redox Homeostasis under Salinity Stress , 2016, Front. Plant Sci..

[4]  K. R. Reddy,et al.  Abscisic Acid and Abiotic Stress Tolerance in Crop Plants , 2016, Front. Plant Sci..

[5]  Chao,et al.  Overexpression of an ABA biosynthesis gene using a stress-inducible promoter enhances drought resistance in petunia , 2015, Horticulture Research.

[6]  F. Ma,et al.  Cloning and expression of two 9-cis-epoxycarotenoid dioxygenase genes during fruit development and under stress conditions from Malus , 2014, Molecular Biology Reports.

[7]  S. H. Wani,et al.  Biotechnology and Abiotic Stress Tolerance in Rice , 2014 .

[8]  Jing Zhang,et al.  Exogenous ABA induces drought tolerance in upland rice: the role of chloroplast and ABA biosynthesis-related gene expression on photosystem II during PEG stress , 2014, Acta Physiologiae Plantarum.

[9]  B. Teh,et al.  Abscisic acid perception and signaling: structural mechanisms and applications , 2014, Acta Pharmacologica Sinica.

[10]  R. Mehrotra,et al.  Abscisic acid and abiotic stress tolerance - different tiers of regulation. , 2014, Journal of plant physiology.

[11]  Yang-Dong Guo,et al.  The role of abscisic acid in fruit ripening and responses to abiotic stress. , 2013, Journal of experimental botany.

[12]  I. Hwang,et al.  ABA homeostasis and signaling involving multiple subcellular compartments and multiple receptors , 2013, Plant Cell Reports.

[13]  Zhanjing Huang,et al.  A Novel ABA-Responsive TaSRHP Gene from Wheat Contributes to Enhanced Resistance to Salt Stress in Arabidopsis thaliana , 2013, Plant Molecular Biology Reporter.

[14]  J. Bailey-Serres,et al.  Waterproofing Crops: Effective Flooding Survival Strategies1 , 2012, Plant Physiology.

[15]  Jukon Kim,et al.  Characterization of the stress-inducible OsNCED3 promoter in different transgenic rice organs and over three homozygous generations , 2012, Planta.

[16]  David Seung,et al.  Circadian clock-dependent gating in ABA signalling networks , 2012, Protoplasma.

[17]  R. Beachy,et al.  Induction of 9-cis-epoxycarotenoid dioxygenase in Arabidopsis thaliana seeds enhances seed dormancy , 2011, Proceedings of the National Academy of Sciences.

[18]  J. Schroeder,et al.  Evolution of Abscisic Acid Synthesis and Signaling Mechanisms , 2011, Current Biology.

[19]  H. Rolletschek,et al.  ABA biosynthesis and degradation contributing to ABA homeostasis during barley seed development under control and terminal drought-stress conditions. , 2011, Journal of experimental botany.

[20]  Jianhua Zhang,et al.  ABA controls H₂O₂ accumulation through the induction of OsCATB in rice leaves under water stress. , 2011, Plant & cell physiology.

[21]  M. Aftab,et al.  Cloning and Expression , 2011 .

[22]  K. Venkatesan,et al.  Restoration of saline land by halophytes for Indian soils , 2007 .

[23]  D. Kim,et al.  Transgenic Plants of Creeping Bent Grass Harboring the Stress Inducible Gene, 9-cis-epoxycarotenoid dioxygenase, are Highly Tolerant to Drought and NaCl Stress , 2005, Plant Growth Regulation.

[24]  E. Nambara,et al.  Abscisic acid biosynthesis and catabolism. , 2005, Annual review of plant biology.

[25]  K. Cline,et al.  Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family. , 2003, The Plant journal : for cell and molecular biology.

[26]  B. Milborrow.  The pathway of biosynthesis of abscisic acid in vascular plants: a review of the present state of knowledge of ABA biosynthesis. , 2001, Journal of experimental botany.

[27]  J. Zeevaart,et al.  Characterization of the 9-cis-epoxycarotenoid dioxygenase gene family and the regulation of abscisic acid biosynthesis in avocado. , 2000, Plant physiology.

[28]  A. Thompson,et al.  Abscisic acid biosynthesis in tomato: regulation of zeaxanthin epoxidase and 9-cis-epoxycarotenoid dioxygenase mRNAs by light/dark cycles, water stress and abscisic acid , 2000, Plant Molecular Biology.

[29]  D. McCarty,et al.  Characterization of the ABA-deficient tomato mutant notabilis and its relationship with maize Vp14. , 1999, The Plant journal : for cell and molecular biology.

[30]  D. McCarty,et al.  Specific oxidative cleavage of carotenoids by VP14 of maize. , 1997, Science.

[31]  Dekan der Mathematisch-Naturwissenschaftlichen,et al.  Functional characterization of , 2014 .

[32]  Peter J. Schaap,et al.  Molecular characterization of the , 1997 .