Isolation and Identification of Inhibitory Compounds from Morus alba cv. Kuksang on α-amylase and α-glucosidase

The objective of this research was to evaluate the inhibitory activities of phenolic compounds isolated from mulberry (Morus alba) leaves of 109 types against α-amylase and α-glucosidase. The inhibitory activity of the water extracts from Morus alba cv. Kuksang against α-amylase and α-glucosidase were determined as 93.8% and 48.7% respectively. The total phenolic content of extracts from Morus alba cv. Kuksang was 9.7±0.2 mg/g soluble in water and 14.3±0.2 mg/g soluble in ethanol. The inhibitory activity of the water extracts from Morus alba cv. Kuksang at 200 μg/ml phenolics concentration against α-amylase and α-glucosidase were determined as 100% and 82.6% respectively. The purification of inhibitory compounds was carried out by Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient elution procedure by nomal phase type (EtOH→distilled water) and reverse phase type (distilled water→MeOH). The quercetin was confirmed to be the chemical structure of the inhibitory compound against α-amylase and α-glucosidase by spectroscopic analysis of FAB-MS, NMR and IR spectrum.

[1]  Moon,et al.  A Study on The Relation between Psychological Stress and Stress Hormone, Nutritional Status of Patients with Non-Insulin Dependent Diabetes Mellitus , 1996 .

[2]  R. Armon,et al.  In vitro inhibition of Helicobacter pylori by extracts of thyme. , 1996, The Journal of applied bacteriology.

[3]  G. Reeck,et al.  alpha-Amylase inhibitors from wheat: amino acid sequences and patterns of inhibition of insect and human alpha-amylases. , 1996, Insect biochemistry and molecular biology.

[4]  N. Markwick,et al.  α-Amylase Activities in Larval Midgut Extracts from Four Species of Lepidoptera (Tortricidae and Gelechiidae): Response to pH and to Inhibitors from Wheat, Barley, Kidney Bean, and Streptomyces , 1996 .

[5]  P. Midolo,et al.  In vitro inhibition of Helicobacter pylori NCTC 11637 by organic acids and lactic acid bacteria. , 1995, The Journal of applied bacteriology.

[6]  G. Hasçelik,et al.  The bactericidal activity of tea against Helicobacter pylori , 1994 .

[7]  N. G. Nair,et al.  Lactobacillus acidophilus inhibits growth of Campylobacter pylori in vitro , 1989, Journal of clinical microbiology.

[8]  G. Nonaka.  Isolation and structure elucidation of tannins , 1989 .

[9]  T. Matsuo,et al.  The Chemical Structure of Kaki-tannin from Immature Fruit of the Persimmon (Diospyros kaki L.) , 1978 .

[10]  M. S. Blois,et al.  Antioxidant Determinations by the Use of a Stable Free Radical , 1958, Nature.

[11]  H. Fuwa.  A NEW METHOD FOR MICRODETERMINATION CF AMYLASE ACTIVITY BY THE USE OF AMYLOSE AS THE SUBSTRATE , 1954 .

[12]  A. C. Hulme.  The isolation of chlorogenic acid from the apple fruit. , 1953, The Biochemical journal.

[13]  R. Skadsen,et al.  Molecular cloning and characterization of a gibberellin-inducible, putative α-glucosidase gene from barley , 2004, Plant Molecular Biology.

[14]  김선재,et al.  Antimicrobial Effect of Methanol Extracts from Some Medicinal Herbs and the Content of Phenolic Compounds , 2004 .

[15]  Rhee,et al.  Inhibitory Effects of Proanthocyanidin Extracted from Distylium racemosum on α-Amylase and α-Glucosidase Activities , 2004 .

[16]  De Pratt,et al.  Phenolic compounds in food and their effects on health II , 1992 .

[17]  L. G. Ensminger.  The Association of Official Analytical Chemists , 1976 .