Effects of Temperature and Illuminating Conditions on Regeneration and Development of Bulblets in Scale Culture of Seven Lilium Spp.

Excised scales of lily bulbs were kept at 15°C and 25°C, and under continuous light or dark to study the regeneration and development of bulblets. The seven species were examined : Lilium rubellum Baker, L. speciosum Thunb. 'Uchida', L. nobilissimum Makino, L. formosanum Wallace, L. longiflorum Thunb. 'Hinomoto', L. maculatum Thunb., and the Asiatic hybrid L. X 'Benisugata'. 1. Generally, more bulblets were regenerated at 25°C than at 15°C in all Lilium spp : bulblets of L. rubellum and L. X 'Benisugata' were formed equally well at 15°C and 25°C. Regardless of temperatures, more bulblets of L. formosanum, L. longiflorum 'Hinomoto', and L. X 'Benisugata' regenerated in the light more than they did in the dark. 2. Regenerated bulblets grew better under light at 25°C than at 15°C and the light stimulated the formation of scaly leaves from bulblets of all species and cultivars, except in L. nobilissimum. In L. nobilissimum, bulblets failed to form scaly leaves under any cultural conditions. Growth of bulblets of L. formosanum, L. longiflorum 'Hinomoto', and L. maculatum was promoted in darkness, whereas the bulblets of L. rubellum and L. nobilissimum grew best under light at 25°C. 3. Bulblets regenerated at 15°C tended to rot during cold treatments compared with those regenerated at 25°C, and the latter bulblets sprouted more frequently than the former ones after transplantation in a greenhouse.

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