Profiling the phenolic compounds of Artemisia pectinata by HPLC-PAD-MSn.

An HPLC-PAD-MS(n) method was employed to profile the phenolic compounds of the aerial part of Artemisia pectinata (Neopallasia pectinata), a plant with no previous reports concerning its phenolic constituents. Three isomers of trans-caffeoylquinic acid accompanied by cis-5-caffeoylquinic acid, six isomers of trans-dicaffeoylquinic acid, two isomers of methyl trans-dicaffeoylquinate (including one new isomer), a trans-caffeoylferuloylquinic acid and three flavanoids were identified unambiguously by analysis of their UV and MS(n) spectra in comparison with standard compounds that were isolated from natural sources, or synthesised, or were surrogate standards (green coffee extract). Other compounds were identified by analysis of their UV and MSn data in comparison with those reported in the literature. MS(n) experiments also suggested the presence of groups of dicaffeoylquinic acid glycosides, caffeoylquinic acid diglycosides, caffeoylquinic acid glycosides and quinic acid diglycosides.

[1]  J. Harnly,et al.  A screening method for the identification of glycosylated flavonoids and other phenolic compounds using a standard analytical approach for all plant materials. , 2007, Journal of agricultural and food chemistry.

[2]  M. Clifford,et al.  Profiling the chlorogenic acids and other caffeic acid derivatives of herbal chrysanthemum by LC-MSn. , 2007, Journal of agricultural and food chemistry.

[3]  M. Clifford,et al.  Profiling the chlorogenic acids of aster by HPLC-MS(n). , 2006, Phytochemical analysis : PCA.

[4]  K. Kubomura,et al.  Identification of the Major Polyphenols in Boysenberry Leaves and Their Suppressive Effect on Carbon Tetrachloride-Induced Liver Injury in Mice , 2006 .

[5]  M. Clifford,et al.  Discriminating between the six isomers of dicaffeoylquinic acid by LC-MS(n). , 2005, Journal of agricultural and food chemistry.

[6]  A. Suksamrarn,et al.  Antimycobacterial activity and cytotoxicity of flavonoids from the flowers ofChromolaena odorata , 2004, Archives of pharmacal research.

[7]  M. Claeys,et al.  Mass spectrometry in the structural analysis of flavonoids. , 2004, Journal of mass spectrometry : JMS.

[8]  D. Ferreira,et al.  Antioxidant effect of flavonoids on DCF production in HL‐60 cells , 2003, Phytotherapy research : PTR.

[9]  Carles Codina,et al.  Identification of phenolic compounds in artichoke waste by high-performance liquid chromatography-tandem mass spectrometry. , 2003, Journal of chromatography. A.

[10]  M. Clifford,et al.  Hierarchical scheme for LC-MSn identification of chlorogenic acids. , 2003, Journal of agricultural and food chemistry.

[11]  A. Galal,et al.  O-demethylation and sulfation of 7-methoxylated flavanones by Cunninghamella elegans. , 2003, Chemical & pharmaceutical bulletin.

[12]  L. Watson,et al.  Molecular phylogeny of Subtribe Artemisiinae (Asteraceae), including Artemisia and its allied and segregate genera , 2002, BMC Evolutionary Biology.

[13]  N. Nakamura,et al.  Inhibitory effects on HIV-1 protease of tri-p-coumaroylspermidine from Artemisia caruifolia and related amides. , 2001, Chemical & pharmaceutical bulletin.

[14]  W. Robinson,et al.  New and facile method of preparation of the anti-HIV-1 agent, 1,3-dicaffeoylquinic acid , 2001 .

[15]  K. Hase,et al.  Four di-O-caffeoyl quinic acid derivatives from propolis. Potent hepatoprotective activity in experimental liver injury models. , 1996, Biological & pharmaceutical bulletin.

[16]  Q. Jia,et al.  Dicaffeoylquinic acid inhibitors of human immunodeficiency virus integrase: inhibition of the core catalytic domain of human immunodeficiency virus integrase. , 1996, Molecular pharmacology.

[17]  Q. Jia,et al.  Inhibitors of HIV-1 replication that inhibit HIV integrase , 1996 .

[18]  N. B. Perry,et al.  Antiviral and antifungal flavonoids, plus a triterpene, from Hebe cupressoides. , 1994, Planta medica.