The use of FTA cards for preserving unfixed cytological material for high‐throughput molecular analysis

Novel high‐throughput molecular technologies have made the collection and storage of cells and small tissue specimens a critical issue. The FTA card provides an alternative to cryopreservation for biobanking fresh unfixed cells. The current study compared the quality and integrity of the DNA obtained from 2 types of FTA cards (Classic and Elute) using 2 different extraction protocols (“Classic” and “Elute”) and assessed the feasibility of performing multiplex mutational screening using fine‐needle aspiration (FNA) biopsy samples.

[1]  L. Staudt,et al.  Mechanisms of Disease , 2010 .

[2]  G. da Cunha Santos,et al.  Maximizing the yield of lymph node cytology , 2011, Cancer cytopathology.

[3]  P. Wesseling,et al.  Preparing pathology for personalized medicine: possibilities for improvement of the pre‐analytical phase , 2011, Histopathology.

[4]  C. Flowers,et al.  Novel agents for diffuse large B-cell lymphoma , 2011, Expert opinion on investigational drugs.

[5]  M. Tsao,et al.  Detection of EGFR and KRAS mutations in fine‐needle aspirates stored on Whatman FTA cards , 2010, Cancer cytopathology.

[6]  P. Meltzer,et al.  Archival fine-needle aspiration cytopathology (FNAC) samples: untapped resource for clinical molecular profiling. , 2010, The Journal of molecular diagnostics : JMD.

[7]  G. da Cunha Santos,et al.  Targeted use of fluorescence in situ hybridization (FISH) in cytospin preparations , 2010, Cancer cytopathology.

[8]  G. da Cunha Santos,et al.  Classifying B‐cell non‐Hodgkin lymphoma by using MIB‐1 proliferative index in fine‐needle aspirates , 2010, Cancer cytopathology.

[9]  S. Aparicio,et al.  Does massively parallel DNA resequencing signify the end of histopathology as we know it? , 2010, The Journal of pathology.

[10]  S. Paik,et al.  A rapid, sensitive, reproducible and cost-effective method for mutation profiling of colon cancer and metastatic lymph nodes , 2010, BMC Cancer.

[11]  Ulf Gyllensten,et al.  Use of FTA card for dry collection, transportation and storage of cervical cell specimen to detect high-risk HPV. , 2009, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology.

[12]  W. Quint,et al.  Detection and Genotyping of Human Papillomavirus in Self-Obtained Cervicovaginal Samples by Using the FTA Cartridge: New Possibilities for Cervical Cancer Screening , 2009, Journal of Clinical Microbiology.

[13]  Iúri Drumond Louro,et al.  Simplified buccal DNA extraction with FTA Elute Cards. , 2009, Forensic science international. Genetics.

[14]  S. Boerner,et al.  Equivalence of laser scanning cytometric and flow cytometric immunophenotyping of lymphoid lesions in cytologic samples. , 2008, American journal of clinical pathology.

[15]  F. Nielsen,et al.  Collection of Blood, Saliva, and Buccal Cell Samples in a Pilot Study on the Danish Nurse Cohort: Comparison of the Response Rate and Quality of Genomic DNA , 2007, Cancer Epidemiology Biomarkers & Prevention.

[16]  S. Mas,et al.  DNA cards: determinants of DNA yield and quality in collecting genetic samples for pharmacogenetic studies. , 2007, Basic & clinical pharmacology & toxicology.

[17]  Hongbin He,et al.  Improved technique that allows the performance of large-scale SNP genotyping on DNA immobilized by FTA technology. , 2007, Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases.

[18]  A. Lopes,et al.  The feasibility of storing ovarian tumor cells on databasing paper: establishing a library of ovarian cancer DNA , 2006, International Journal of Gynecologic Cancer.

[19]  R. Scott,et al.  Buccal DNA Collection: Comparison of Buccal Swabs with FTA Cards , 2006, Cancer Epidemiology Biomarkers & Prevention.

[20]  Jitender Yadav,et al.  Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues , 2005, Virology Journal.

[21]  Alexis B. Carter,et al.  Use of FTA gene guard filter paper for the storage and transportation of tumor cells for molecular testing. , 2002, Archives of pathology & laboratory medicine.

[22]  D. Fox,et al.  Paper-based archiving of mammalian and plant samples for RNA analysis. , 2000, BioTechniques.

[23]  P. Chomczyński,et al.  Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. , 1997, BioTechniques.