The influence of chitin structure on its enzymatic deacetylation

The possibility of producing chitosan by enzymatic deacetylation of chitin has been the subject of numerous investigations over the last twenty years, but to date no satisfactory method has been developed. In this paper the influence of chitin chain conformation and chitin particle crystallinity on the enzymatic deacetylation of chitin is investigated to determine the relative importance of these two factors on the process. It is shown that the high crystallinity of chitin is the main obstacle to converting chitin to chitosan by enzymatic deacetylation.

[1]  A. Górak,et al.  Review of the application of ionic liquids as solvents for chitin , 2012 .

[2]  M. Jaworska.  Kinetics of enzymatic deacetylation of chitosan , 2012, Cellulose.

[3]  J. Kennedy,et al.  Purification and characterization of chitin deacetylase from Scopulariopsis brevicaulis , 2006 .

[4]  Suobo Zhang,et al.  Chitin and chitosan dissolved in ionic liquids as reversible sorbents of CO2 , 2006 .

[5]  M. Rinaudo,et al.  Chitin and chitosan: Properties and applications , 2006 .

[6]  T. Ahamed,et al.  Peripheral enzymatic deacetylation of chitin and reprecipitated chitin particles. , 2006, Bioresource technology.

[7]  C. Alfonso,et al.  Purification of a heat-stable chitin deacetylase from Aspergillus nidulans and its role in cell wall degradation , 2004, Current Microbiology.

[8]  M. Jaworska,et al.  Influence of chitosan characteristics on polymer properties. I: Crystallographic properties , 2003 .

[9]  W. Stevens,et al.  Shrimp chitin as substrate for fungal chitin deacetylase , 2001, Applied Microbiology and Biotechnology.

[10]  M. Jaworska,et al.  The influence of supplemental components in nutrient medium on chitosan formation by the fungus Absidia orchidis , 2001, Applied Microbiology and Biotechnology.

[11]  D Kafetzopoulos,et al.  Chitin deacetylases: new, versatile tools in biotechnology. , 2000, Trends in biotechnology.

[12]  K. Tokuyasu,et al.  Purification and characterization of extracellular chin deacetylase from Colletotrichum lindemuthianum. , 1996, Bioscience, biotechnology, and biochemistry.

[13]  Dimitris Kafetzopoulos,et al.  Chitin deacetylation by enzymatic means: monitoring of deacetylation processes , 1995 .

[14]  C. A. Smolders,et al.  Surface amination of poly(acrylonitrile) , 1994 .

[15]  J. Arul,et al.  Effect of chitosan and other polyions on chitin deacetylase inRhizopus stolonifer , 1992 .