Laser Speckle Contrast Imaging of Collateral Blood Flow during Acute Ischemic Stroke

Collateral vasculature may provide an alternative route for blood flow to reach the ischemic tissue and partially maintain oxygen and nutrient support during ischemic stroke. However, much about the dynamics of stroke-induced collateralization remains unknown. In this study, we used laser speckle contrast imaging to map dynamic changes in collateral blood flow after middle cerebral artery occlusion in rats. We identified extensive anastomatic connections between the anterior and middle cerebral arteries that develop after vessel occlusion and persist for 24 hours. Augmenting blood flow through these persistent yet dynamic anastomatic connections may be an important but relatively unexplored avenue in stroke therapy.

[1]  M. Moskowitz,et al.  Dynamic Imaging of Cerebral Blood Flow Using Laser Speckle , 2001, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[2]  D. Liebeskind.  Collateral circulation. , 2003, Stroke.

[3]  K. Hossmann.  Pathophysiology and Therapy of Experimental Stroke , 2006, Cellular and Molecular Neurobiology.

[4]  W. J. Tom,et al.  Robust flow measurement with multi-exposure speckle imaging. , 2008, Optics express.

[5]  R. Uflacker,et al.  Interim report of the SENTIS trial: cerebral perfusion augmentation via partial aortic occlusion in acute ischemic stroke. , 2008, The Journal of cardiovascular surgery.

[6]  G. Paxinos,et al.  The Rat Brain in Stereotaxic Coordinates , 1983 .

[7]  Donald D Duncan,et al.  Can laser speckle flowmetry be made a quantitative tool? , 2008, Journal of the Optical Society of America. A, Optics, image science, and vision.

[8]  W. Schaper,et al.  Collateral Circulation , 1993, Springer US.

[9]  A. Green.  Pharmacological approaches to acute ischaemic stroke: reperfusion certainly, neuroprotection possibly , 2008, British journal of pharmacology.

[10]  M. Moskowitz,et al.  Laser Speckle Flowmetry for the Study of Cerebrovascular Physiology in Normal and Ischemic Mouse Cortex , 2004, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[11]  T. Murphy,et al.  Two-Photon Imaging during Prolonged Middle Cerebral Artery Occlusion in Mice Reveals Recovery of Dendritic Structure after Reperfusion , 2008, The Journal of Neuroscience.

[12]  D. Liebeskind.  Collaterals in acute stroke: beyond the clot. , 2005, Neuroimaging clinics of North America.

[13]  David A. Boas,et al.  Mild Induced Hypertension Improves Blood Flow and Oxygen Metabolism in Transient Focal Cerebral Ischemia , 2008, Stroke.

[14]  J. Wahr,et al.  Partial Intra‐Aortic Occlusion Improves Perfusion Deficits and Infarct Size Following Focal Cerebral Ischemia , 2009, Journal of neuroimaging : official journal of the American Society of Neuroimaging.

[15]  K. Todd,et al.  Patency of Cerebral Microvessels after Focal Embolic Stroke in the Rat , 2001, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[16]  F Viñuela,et al.  Impact of collateral flow on tissue fate in acute ischaemic stroke , 2007, Journal of Neurology, Neurosurgery, and Psychiatry.