Targeted genome modification of crop plants using a CRISPR-Cas system

1. Jinek, M. et al. Science 337, 816–821 (2012). 2. Cho, S.W., Kim, S., Kim, J.M. & Kim, J.S. Nat. Biotechnol. 31, 230–232 (2013). 3. Cong, L. et al. Science 339, 819–823 (2013). 4. Mali, P. et al. Science 339, 823–826 (2013). 5. Hwang, W.Y. et al. Nat. Biotechnol. 31, 227–229 (2013). 6. Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L.A. Nat. Biotechnol. 31, 233–239 (2013). 7. Wang, H. et al. Cell 153, 910–918 (2013). 8. Geurts, A.M. et al. Science 325, 433 (2009). 9. Tong, C., Li, P., Wu, N.L., Yan, Y. & Ying, Q.L. Nature 467, 211–213 (2010). 10. Tesson, L. et al. Nat. Biotechnol. 29, 695–696 (2011). 11. Wu, H. & Zhang, Y. Genes Dev. 25, 2436–2452 (2011). 12. Gu, T.P. et al. Nature 477, 606–610 (2011). 13. Dawlaty, M.M. et al. Dev. Cell 24, 310–323 (2013). revision process of this work, an independent study reported the simultaneous generation of multiple mutations in mice7. Our work, together with the mice work, demonstrates that it should be feasible to produce genetargeted models in rodents and probably other mammalian species using the CRISPRCas systems.

[1]  J. Doudna,et al.  A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity , 2012, Science.

[2]  Jeffry D. Sander,et al.  Efficient In Vivo Genome Editing Using RNA-Guided Nucleases , 2013, Nature Biotechnology.

[3]  Feng Zhang,et al.  CRISPR-assisted editing of bacterial genomes , 2013, Nature Biotechnology.

[4]  R. Barrangou,et al.  CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes , 2007, Science.

[5]  Kunling Chen,et al.  TALENs: customizable molecular DNA scissors for genome engineering of plants. , 2013, Journal of genetics and genomics = Yi chuan xue bao.

[6]  Xiaojun Zhu,et al.  Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos , 2013, Cell Research.

[7]  J. Keith Joung,et al.  High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells , 2013, Nature Biotechnology.

[8]  Feng Zhang,et al.  High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases , 2010, Proceedings of the National Academy of Sciences.

[9]  R. Barrangou,et al.  Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria , 2012, Proceedings of the National Academy of Sciences.

[10]  D. Voytas,et al.  Rapid and efficient gene modification in rice and Brachypodium using TALENs. , 2013, Molecular plant.

[11]  Rudolf Jaenisch,et al.  One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering , 2013, Cell.

[12]  T. Cathomen,et al.  RNA guides genome engineering , 2013, Nature Biotechnology.

[13]  Le Cong,et al.  Multiplex Genome Engineering Using CRISPR/Cas Systems , 2013, Science.