Amino acid and hexose transport of normal and simian virus 40-transformed human cells.

Studies on amino acid and hexose transport were performed on human WI-38 cells and WI-38 SV40-transformed cells (VA13A and VA13-2RA). Depending upon cell line or conditions, either no difference or a relative decrease in initial uptake by transformed cells was found. Under similar growth conditions, transformed hamster cells (PyBHK-21/C13) had increased uptake, compared with the normal hamster cells (BHK-21/C13). The normal and transformed human cells were also similar in sialic acid content and agglutinated when treated with concanavalin A.

[1]  D. Wallach,et al.  Amino Acid and Sugar Transport in Cells Permissively Infected with Simian Virus 40 , 1975 .

[2]  J. Pontén,et al.  Lectin agglutinability of non‐neoplastic and neoplastic human lymphoid cells in vitro , 1975, International journal of cancer.

[3]  M. Siddiqi,et al.  Studies on the uptake of 2‐deoxy‐d‐glucose in normal and malignant rat epithelial liver cells in culture , 1975, International journal of cancer.

[4]  S. Venuta,et al.  Effects of glucose starvation on normal and rous sarcoma virus-transformed chick cells. , 1975, Journal of the National Cancer Institute.

[5]  A. H. Romano,et al.  Phosphorylation but not transport of sugars is enhanced in virus‐transformed mouse 3T3 cells , 1975, Journal of cellular physiology.

[6]  R. Kletzien,et al.  Induction of sugar transport in chick embryo fibroblasts by hexose starvation. Evidence for transcriptional regulation of transport. , 1975, The Journal of biological chemistry.

[7]  M. Patterson.  Alterations in membrane structure and function associated with neoplastic transformation in vitro. , 1974, Journal of the National Cancer Institute.

[8]  J. Pontén,et al.  Agglutination of normal and neoplastic human cells by concanavalin A and ricinus communis agglutinin , 1974, International journal of cancer.

[9]  M Hatanaka,et al.  Transport of sugars in tumor cell membranes. , 1974, Biochimica et biophysica acta.

[10]  Toshio Kuroki,et al.  Kinetics of uptake of 2‐deoxy‐d‐glucose and 2‐aminoisobutyric acid in chemically transformed cells , 1974, International journal of cancer.

[11]  S. Bose,et al.  Growth-and density-dependent inhibition of deoxyglucose transport in Balb 3T3 cells and its absence in cells transformed by murine sarcoma virus. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M. Weber.  Hexose transport in normal and in Rous sarcoma virus-transformed cells. , 1973, The Journal of biological chemistry.

[13]  R. Huebner,et al.  Problems in interpretation of experimental evidence of cell transformation. , 1973, Journal of the National Cancer Institute.

[14]  K. Isselbacher.  Increased uptake of amino acids and 2-deoxy-D-glucose by virus-transformed cells in culture. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[15]  A. Pardee,et al.  Transport of amino acids by confluent and nonconfluent 3T3 and polyoma virus-transformed 3T3 cells growing on glass cover slips. , 1969, The Journal of biological chemistry.

[16]  L. Rosenberg,et al.  Extracellular space estimation in rat kidney slices using C saccharides and phlorizin. , 1962, The American journal of physiology.

[17]  K. Piez,et al.  The intracellular amino acid concentrations required for protein synthesis in cultured human cells. , 1961, The Journal of biological chemistry.

[18]  Oliver H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[19]  M. Barile.  Mycoplasma Contamination of Cell Cultures: Incidence, Source, Prevention, and Problems of Elimination , 1973 .