Spatial coding of olfactory information in the antennal lobe of Drosophila melanogaster

[3H]2-deoxyglucose autoradiography has revealed foci in the antennal lobe of Drosophila melanogaster that are active during olfactory stimulation. Labelling in the receptor axons and the lobe interneurons can be observed. Each class of odors stimulates activity in a specific subset of antennal glomeruli. This defines the activity domain, which is recognizably distinct, although overlapping, for different classes of volatile chemicals. Unilateral stimulation with attractants predominantly results in excitation of the ipsilateral glomeruli. Collaterals of the receptor neurons to the contralateral lobe stimulate no visible postsynaptic activity. On the other hand, unilateral stimulation with repellent odors, such as benzaldehyde, results in an equally strong activity in both antennal lobes.

[1]  Martin H. Teicher,et al.  Functional development of the olfactory bulb and a unique glomerular complex in the neonatal rat , 1982, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[2]  I. Bülthoff,et al.  Freeze-substitution of Drosophila heads for subsequent [3H]2-deoxyglucose autoradiography , 1985, Journal of Neuroscience Methods.

[3]  M. Reivich,et al.  THE [14C]DEOXYGLUCOSE METHOD FOR THE MEASUREMENT OF LOCAL CEREBRAL GLUCOSE UTILIZATION: THEORY, PROCEDURE, AND NORMAL VALUES IN THE CONSCIOUS AND ANESTHETIZED ALBINO RAT 1 , 1977, Journal of neurochemistry.

[4]  R. Chase,et al.  Responses to odors mapped in snail tentacle and brain by [14C]-2- deoxyglucose autoradiography , 1985, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[5]  J S Kauer,et al.  Mapping of odor-related neuronal activity in the olfactory bulb by high-resolution 2-deoxyglucose autoradiography. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[6]  M. E. Power.  The antennal centers and their connections within the brain of Drosophila melanogaster , 1946, The Journal of comparative neurology.

[7]  E. Adrian,et al.  Sensory messages and sensation; the response of the olfactory organ to different smells. , 1953, Acta physiologica Scandinavica.

[8]  G. Shepherd,et al.  Functional organization of rat olfactory bulb analysed by the 2‐deoxyglucose method , 1979, The Journal of comparative neurology.

[9]  L. Astic,et al.  Spatial distribution of [14C]2-deoxyglucose uptake in the olfactory bulbs of rats stimulated with two different odours , 1980, Brain Research.

[10]  E. Buchner,et al.  Anatomical Localization of Functional Activity in Flies Using 3H-2-Deoxy-d-Glucose , 1983 .

[11]  V. Rodrigues.  Olfactory behavior of Drosophila melanogaster. , 1980, Basic life sciences.

[12]  Erich Buchner,et al.  [3H]2-Deoxyglucose mapping of odor-induced neuronal activity in the antennal lobes of Drosophila melanogaster , 1984, Brain Research.

[13]  O. Siddiqi.  Neurogenetics of olfaction in Drosophila melanogaster , 1987 .

[14]  N. Strausfeld.  Atlas of an Insect Brain , 1976, Springer Berlin Heidelberg.

[15]  H. K. Hartline,et al.  INHIBITORY INTERACTION OF RECEPTOR UNITS IN THE EYE OF LIMULUS , 1957, The Journal of general physiology.

[16]  Martin H. Teicher,et al.  Suckling pheromone stimulation of a modified glomerular region in the developing rat olfactory bulb revealed by the 2-deoxyglucose method , 1980, Brain Research.

[17]  J. Rospars,et al.  The deutocerebrum of the cockroach Blaberus craniifer Burm. Spatial organization of the sensory glomeruli. , 1980, Journal of neurobiology.

[18]  F R Sharp,et al.  Laminar analysis of 2-deoxyglucose uptake in olfactory bulb and olfactory cortex of rabbit and rat. , 1977, Journal of neurophysiology.

[19]  J. Hildebrand,et al.  Olfactory interneurons in the moth Manduca sexta: response characteristics and morphology of central neurons in the antennal lobes , 1981, Proceedings of the Royal Society of London. Series B. Biological Sciences.