Apparent diffusion coefficient: a quantitative parameter for in vivo tumor characterization.

PURPOSE The purpose of the this study was to evaluate the potential of diffusion weighted imaging (DWI) to distinguish different tissue compartments in early, intermediate and advanced tumor stages. MATERIALS AND METHODS Twenty-two male mice were induced with squamous cell tumor (SCCVII) and scanned with a clinical 1.5 T scanner. T1-SE, T2-FSE, diffusion weighted Line-Scan-MRI and contrast enhanced T1-SE were obtained from mice with early (tumor volume 10-100 mm(3)), intermediate (200-600 mm(3)), advanced tumors (600-1000 mm(3)) and tumor necrosis (>1500 mm(3)). The apparent diffusion coefficient (ADC) of different tumor compartments was calculated offline with a pixel-by-pixel method. The animals were sacrificed immediately after scanning and histopathologic correlation was performed. RESULTS In early stages of tumor development, tumors appeared homogeneous on diffusion weighted images with an ADC of 0.64+/-0.06 x 10(-3) mm(2)/s. With tumor progression the ADC in the rim areas of tumor increased significantly (intermediate stage: 0.70+/-0.11 x 10(-3) mm(2)/s; advanced stage: 0.88+/-0.11 x 10(-3) mm(2)/s; tumor necrosis 1.03+/-0.06 x 10(-3) mm(2)/s), whereas the ADC in viable tumor remained constant. Histologically the areas with an increased ADC correlated well with areas of necrosis (reduced cell density). CONCLUSION The ADC is a non-invasive technique to monitor changes in the biological structure of tumor tissue during tumor progression. Thus, DWI is a potential diagnostic tool for in-vivo tissue characterization.

[1]  D. Bihan,et al.  Differentiation of benign versus pathologic compression fractures with diffusion-weighted MR imaging: a closer step toward the "holy grail" of tissue characterization? , 1998 .

[2]  A. Herneth,et al.  Wertigkeit der diffusionsgewichteten MRT in der Beurteilung von Knochenmarkveränderungen bei Wirbelkörpermetastasen , 2000, Der Radiologe.

[3]  A. J. Kumar,et al.  Magnetic resonance imaging of benign spinal lesions simulating metastasis: role of diffusion-weighted imaging. , 2000, Topics in magnetic resonance imaging : TMRI.

[4]  Olav Haraldseth,et al.  Measurement of cell density and necrotic fraction in human melanoma xenografts by diffusion weighted magnetic resonance imaging , 2000, Magnetic resonance in medicine.

[5]  J. Gore,et al.  Theoretical Model for Water Diffusion in Tissues , 1995, Magnetic resonance in medicine.

[6]  D. Bihan,et al.  Molecular diffusion, tissue microdynamics and microstructure , 1995 .

[7]  Diffusion-weighted imaging of acute vertebral compressions: specific diagnosis of benign versus malignant pathologic fractures. , 2001, AJNR. American journal of neuroradiology.

[8]  H. Ostertag,et al.  Percutaneous bone biopsy, techniques and indications , 2004, European Radiology.

[9]  J C Gore,et al.  Diffusion‐weighted imaging in tissues: Theoretical models , 1995, NMR in biomedicine.

[10]  R. Damadian Tumor Detection by Nuclear Magnetic Resonance , 1971, Science.

[11]  R. Brasch,et al.  Characteristics of gadolinium-DTPA complex: a potential NMR contrast agent. , 1984, AJR. American journal of roentgenology.

[12]  R. Turner,et al.  Diffusion MR imaging: clinical applications. , 1992, AJR. American journal of roentgenology.

[13]  D. Le Bihan,et al.  Separation of diffusion and perfusion in intravoxel incoherent motion MR imaging. , 1988, Radiology.

[14]  L. Hedlund,et al.  Mechanism of Detection of Acute Cerebral Ischemia in Rats by Diffusion‐Weighted Magnetic Resonance Microscopy , 1992, Stroke.

[15]  M Hoehn-Berlage,et al.  High resolution quantitative relaxation and diffusion mri of three different experimental brain tumors in rat , 1995, Magnetic resonance in medicine.

[16]  P. Lang,et al.  Advances in MR imaging of pediatric musculoskeletal neoplasms. , 1998, Magnetic resonance imaging clinics of North America.

[17]  K. Svoboda,et al.  Time-dependent diffusion of water in a biological model system. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[18]  G Adam,et al.  Diffusion-weighted MR imaging for differentiation of benign fracture edema and tumor infiltration of the vertebral body. , 2001, AJR. American journal of roentgenology.

[19]  M Takahashi,et al.  Contrast enhancement of intracranial lesions: conventional T1-weighted spin-echo versus fast spin-echo MR imaging techniques. , 1999, AJNR. American journal of neuroradiology.

[20]  M. Gaeta,et al.  CT and MRI findings of mucin-containing tumors and pseudotumors of the thorax: pictorial review , 2002, European Radiology.

[21]  M. Wendland,et al.  Osteogenic sarcoma: noninvasive in vivo assessment of tumor necrosis with diffusion-weighted MR imaging. , 1998, Radiology.

[22]  R. Mulholland,et al.  Closed vertebral biopsy. , 1983, The Journal of bone and joint surgery. British volume.

[23]  M. Reiser,et al.  Musculoskeletal neoplasms: static and dynamic Gd-DTPA--enhanced MR imaging. , 1989, Radiology.

[24]  Rakesh K. Jain,et al.  Transport of molecules across tumor vasculature , 2004, Cancer and Metastasis Reviews.