Generation of 10,154 expressed sequence tags from a leafy gametophyte of a marine red alga, Porphyra yezoensis.

A total of 10,154 5'-end expressed sequence tags (EST) were established from the normalized and size-selected cDNA libraries of a marine red alga, Porphyra yezoensis. Among the ESTs, 2140 were unique species, and the remaining 8014 were grouped into 1127 species. Database search of the 3267 non-redundant ESTs by BLAST algorithm showed that the sequences of 1080 species (33.1%) have similarity to those of registered genes from various organisms including higher plants, mammals, yeasts, and cyanobacteria, while 2187 (66.9%) are novel. Codon usage analysis in the coding regions of 101 non-redundant EST groups showing significant similarity to known genes indicated the higher GC contents at the third position of codons (79.4%) than the first (62.2%) and the second position (45.0%), suggesting that the genome has been exposed to high GC pressure during evolution. The sequence data of individual ESTs are available at the web site http://www.kazusa.or.jp/en/plant/porphyra/EST/.

[1]  K. Nakanishi,et al.  AXENIC TISSUE CULTURE AND MORPHOGENESIS IN PORPHYRA YEZOENSIS (BANGIALES, RHODOPHYTA) , 1998 .

[2]  Y. Kitade,et al.  Porphyra monospore system (Bangiales, Rhodophyta): A model for the developmental biology of marine plants , 1998 .

[3]  Y. Nakamura,et al.  Generation of 7137 non-redundant expressed sequence tags from a legume, Lotus japonicus. , 2000, DNA research : an international journal for rapid publication of reports on genes and genomes.

[4]  Y. Nakamura,et al.  A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. , 1999, DNA research : an international journal for rapid publication of reports on genes and genomes.

[5]  M. Hirai,et al.  Expression of a Soybean (Glycine max [L.] Merr.) Seed Storage Protein Gene in Transgenic Arabidopsis thaliana and Its Response to Nutritional Stress and to Abscisic Acid Mutations , 1994, Plant physiology.

[6]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[7]  M. Reith,et al.  A High-Resolution Gene Map of the Chloroplast Genome of the Red Alga Porphyra purpurea. , 1993, The Plant cell.

[8]  T. Watanabe,et al.  Structural features of a gene encoding the vacuolar H+-ATPase c subunit from a marine red alga, Porphyra yezoensis. , 1999, DNA research : an international journal for rapid publication of reports on genes and genomes.

[9]  Eugen C. Buehler,et al.  Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana , 1999, Nature.

[10]  D. Bouchez,et al.  AGO1 defines a novel locus of Arabidopsis controlling leaf development , 1998, The EMBO journal.

[11]  R. Simon,et al.  The CONSTANS gene of arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors , 1995, Cell.

[12]  M. Soares,et al.  Normalization and subtraction: two approaches to facilitate gene discovery. , 1996, Genome research.

[13]  T. Sasaki,et al.  Toward cataloguing all rice genes: large-scale sequencing of randomly chosen rice cDNAs from a callus cDNA library. , 1994, The Plant journal : for cell and molecular biology.

[14]  D. Kapraun,et al.  Karyology and cytophotometric estimation of inter- and intraspecific nuclear DNA variation in four species of Porphyra (Rhodophyta) , 1991 .

[15]  M. Ohme,et al.  Tetrad analysis in conchospore germlings of Porphyra yezoensis (Rhodophyta, Bangiales) , 1988 .

[16]  M. Reith,et al.  ISOLATION AND CHARACTERIZATION OF PHASE‐SPECIFIC COMPLEMENTARY DNAs FROM SPOROPHYTES AND GAMETOPHYTES OF PORPHYRA PURPUREA (RHODOPHYTA) USING SUBTRACTED COMPLEMENTARY DNA LIBRARIES 1 , 1994 .

[17]  Y. Kitade,et al.  A METHOD FOR EXTRACTION OF HIGH MOLECULAR WEIGHT DNA FROM THE MACROALGA PORPHYRA YEZOENSIS (RHODOPHYTA) 1, 2 , 1996 .

[18]  P. Rouzé,et al.  An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana. , 1993, The Plant journal : for cell and molecular biology.

[19]  B. Gregory,et al.  The Arabidopsis dwarf 1 Mutant Is Defective in the Conversion of 24-Methylenecholesterol to Campesterol in Brassinosteroid Biosynthesis 1 , 1999 .

[20]  K. Yamato,et al.  Expressed sequence tags from immature female sexual organ of a liverwort, Marchantia polymorpha. , 1999, DNA research : an international journal for rapid publication of reports on genes and genomes.

[21]  N. Saga,et al.  Cryopreservation of clonal gametophytic thalli of Porphyra (Rhodophyta) , 1996 .