A method for simultaneous echo planar imaging of hyperpolarized ¹³C pyruvate and ¹³C lactate.

A rapid echo planar imaging sequence for dynamic imaging of [1-(13)C] lactate and [1-(13)C] pyruvate simultaneously was developed. Frequency-based separation of these metabolites was achieved by spatial shifting in the phase-encoded direction with the appropriate choice of echo spacing. Suppression of the pyruvate-hydrate and alanine resonances is achieved through an optimized spectral-spatial RF waveform. Signal sampling efficiency as a function of pyruvate and lactate excitation angle was simulated using two site exchange models. Dynamic imaging is demonstrated in a transgenic mouse model, and phantom validations of the RF pulse frequency selectivity were performed.

[1]  Peder E. Z. Larson,et al.  Multiband excitation pulses for hyperpolarized 13C dynamic chemical-shift imaging. , 2008, Journal of magnetic resonance.

[2]  John Kurhanewicz,et al.  Analysis of cancer metabolism by imaging hyperpolarized nuclei: prospects for translation to clinical research. , 2011, Neoplasia.

[3]  P. Jezzard,et al.  Correction for geometric distortion in echo planar images from B0 field variations , 1995, Magnetic resonance in medicine.

[4]  S J Kohler,et al.  Imaging considerations for in vivo 13C metabolic mapping using hyperpolarized 13C‐pyruvate , 2009, Magnetic resonance in medicine.

[5]  Charles H Cunningham,et al.  Spectral–spatial excitation for rapid imaging of DNP compounds , 2011, NMR in biomedicine.

[6]  M. Thaning,et al.  Real-time metabolic imaging. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[7]  Michael Lustig,et al.  Pulse sequence for dynamic volumetric imaging of hyperpolarized metabolic products. , 2008, Journal of magnetic resonance.

[8]  M. Lustig,et al.  Fast dynamic 3D MR spectroscopic imaging with compressed sensing and multiband excitation pulses for hyperpolarized 13C studies , 2011, Magnetic resonance in medicine.

[9]  P. Larson,et al.  Multi-band frequency encoding method for metabolic imaging with hyperpolarized [1-(13)C]pyruvate. , 2011, Journal of magnetic resonance.

[10]  Graham A Wright,et al.  Rapid multislice imaging of hyperpolarized 13C pyruvate and bicarbonate in the heart , 2010, Magnetic resonance in medicine.

[11]  Jürgen Hennig,et al.  Fast multiecho balanced SSFP metabolite mapping of 1H and hyperpolarized 13C compounds , 2009, Magnetic Resonance Materials in Physics, Biology and Medicine.

[12]  John Kurhanewicz,et al.  Analysis of hyperpolarized dynamic 13C lactate imaging in a transgenic mouse model of prostate cancer. , 2010, Magnetic resonance imaging.

[13]  Craig R. Malloy,et al.  Hyperpolarized 13C allows a direct measure of flux through a single enzyme-catalyzed step by NMR , 2007, Proceedings of the National Academy of Sciences.

[14]  K. Nagashima,et al.  Optimum pulse flip angles for multi-scan acquisition of hyperpolarized NMR and MRI. , 2008, Journal of magnetic resonance.

[15]  Ilwoo Park,et al.  Kinetic modeling of hyperpolarized 13C1-pyruvate metabolism in normal rats and TRAMP mice. , 2010, Journal of magnetic resonance.

[16]  Adam B Kerr,et al.  Investigation of tumor hyperpolarized [1‐13C]‐pyruvate dynamics using time‐resolved multiband RF excitation echo‐planar MRSI , 2010, Magnetic resonance in medicine.

[17]  F. Gallagher,et al.  Tumor imaging using hyperpolarized 13C magnetic resonance spectroscopy , 2011, Magnetic resonance in medicine.

[18]  K. Brindle,et al.  Kinetic Modeling of Hyperpolarized 13C Label Exchange between Pyruvate and Lactate in Tumor Cells* , 2011, The Journal of Biological Chemistry.

[19]  Jan H. Ardenkjær-Larsen,et al.  Molecular imaging with endogenous substances , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[20]  Thomas M Grist,et al.  Least‐squares chemical shift separation for 13C metabolic imaging , 2007, Journal of magnetic resonance imaging : JMRI.

[21]  Albert P. Chen,et al.  Compressed sensing for resolution enhancement of hyperpolarized 13C flyback 3D-MRSI. , 2008, Journal of magnetic resonance.

[22]  F. Jolesz,et al.  Gradient-Echo Imaging Considerations for Hyperpolarized 129Xe MR , 1996, Journal of magnetic resonance. Series B.

[23]  R. Matusik,et al.  Prostate cancer in a transgenic mouse. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[24]  J. Ardenkjær-Larsen,et al.  Increase in signal-to-noise ratio of > 10,000 times in liquid-state NMR , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[25]  A. Macovski,et al.  Variable-rate selective excitation , 1988 .

[26]  J. Pauly,et al.  Simultaneous spatial and spectral selective excitation , 1990, Magnetic resonance in medicine.

[27]  G. Radda,et al.  In vivo assessment of pyruvate dehydrogenase flux in the heart using hyperpolarized carbon-13 magnetic resonance , 2008, Proceedings of the National Academy of Sciences.

[28]  John M Pauly,et al.  Hyperpolarized C‐13 spectroscopic imaging of the TRAMP mouse at 3T—Initial experience , 2007, Magnetic resonance in medicine.

[29]  Rebekah McLaughlin,et al.  Magnetization transfer measurements of exchange between hyperpolarized [1‐13C]pyruvate and [1‐13C]lactate in a murine lymphoma , 2010, Magnetic resonance in medicine.

[30]  L Zhao,et al.  Gradientecho imaging considerations for hyperpolarized ^ Xe MR , 1996 .

[31]  Ilwoo Park,et al.  Hyperpolarized 13C magnetic resonance metabolic imaging: application to brain tumors. , 2010, Neuro-oncology.

[32]  D M Spielman,et al.  Optimization of fast spiral chemical shift imaging using least squares reconstruction: Application for hyperpolarized 13C metabolic imaging , 2007, Magnetic resonance in medicine.

[33]  Koichi Oshio,et al.  Phase errors in multi‐shot echo planar imaging , 1994, Magnetic resonance in medicine.

[34]  Jan Henrik Ardenkjaer-Larsen,et al.  Metabolic imaging by hyperpolarized 13C magnetic resonance imaging for in vivo tumor diagnosis. , 2006, Cancer research.

[35]  Albert P. Chen,et al.  Hyperpolarized 13C lactate, pyruvate, and alanine: noninvasive biomarkers for prostate cancer detection and grading. , 2008, Cancer research.