论文信息 - The action of phospholipase A and lipid solvents on Murray Valley encephalitis virus.

The action of phospholipase A and lipid solvents on Murray Valley encephalitis virus.

SUMMARY: Phospholipase A (100 μfg./ml.) acting at 37° for 30 min. decreased the infective titre of crude or purified Murray Valley encephalitis (MVE) virus from 7 or 8 log to less than 1 log. This inactivated virus yielded 2 log of infective ‘ribonucleic acid’ (‘RNA’) when it was treated with phenol. Ether, chloroform or butanol acting at 0–4° each decreased the infectivity of crude or purified MVE virus. Again the inactivated virus yielded ‘RNA’ with an infective titre higher than the titre of the inactivated virus. Infective ‘RNA’ prepared from crude MVE virus was not inactivated by ether, chloroform or butanol. High concentrations of phospholipase A preparations destroyed ‘RNA’, possibly due to traces of contaminating ribo-nuclease. The results suggest that intact viral phospholipid is necessary for infectivity of the virus particle.

G. Ada | S. Anderson

[1] E. North,et al. Factors involved in the action of venoms against bacterial toxins in experimental animals. , 1960, British journal of experimental pathology.

[2] R. Morton. Separation and Purification of Enzymes Associated with Insoluble Particles , 1950, Nature.

[3] C. Andrewes,et al. The susceptibility of viruses to ethyl ether. , 1949, Journal of general microbiology.

[4] W. Hammon. MOSQUITOES AND ENCEPHALITIS IN THE YAKIMA VALLEY, WASHINGTON , 1944 .

[5] W. Reeves,et al. Mosquitoes and Encephalitis in the Yakima Valley, Washington II. Methods for Collecting Arthropods and for Isolating Western Equine and St. Louis Viruses , 1942 .

[6] F. Burnet,et al. The Action of Certain Surface-Active Agents on Viruses. , 1940 .

[7] D. Sharp,et al. Isolation and Properties of the Equine Encephalomyelitis Virus (Eastern Strain) , 1943 .