Quantitative Label-Free Animal Sperm Imaging by Means of Digital Holographic Microscopy

A digital holographic microscope (DHM) has been employed in the retrieval and analysis of morphological images of bovine's sperm cells. Digital holography is a noncontact technique capable of investigating the shape of the sample without altering its characteristics and has been used for the first time in retrieving quantitative morphological information of sperm cells. Different spermatozoa have been analyzed by means of this technique allowing us to obtain 3-D images with precise topographical details and valuable information about morphological defects, provided with biological considerations. Moreover, by making use of a microfluidic system, the digital holographic technique has been employed to analyze unstained spermatozoa in their natural physiological surroundings. Detailed information on morphological images of spermatozoa acquired by DHM is expected to provide a better understanding of various reproductive pathways, which, in turn, can help in improving infertility management. This could constitute the basis of an alternative method for the zoothecnic industry aimed at the investigation of morphological features and the sorting of the motile sperm cells.

[1]  P. Ferraro,et al.  Controlling images parameters in the reconstruction process of digital holograms , 2004, IEEE Journal of Selected Topics in Quantum Electronics.

[2]  J. Berg,et al.  Studies on surface wettability of poly(dimethyl) siloxane (PDMS) and glass under oxygen-plasma treatment and correlation with bond strength , 2005, Journal of Microelectromechanical Systems.

[3]  Koel Chaudhury,et al.  Atomic force microscopy: a powerful tool for high-resolution imaging of spermatozoa , 2005, Journal of nanobiotechnology.

[4]  A. Galli,et al.  Some Variability Factors in the Cytomorphological Analysis of Frozen Bull Semen/Einige Variabilitäts‐Faktoren der cytomorphologischen Untersuchung von tiefgefrorenem Bullensperma , 2009, Andrologia.

[5]  Chun-Min Lo,et al.  High-resolution quantitative phase-contrast microscopy by digital holography. , 2005, Optics express.

[6]  Bahram Javidi,et al.  Extended focused image in microscopy by digital Holography. , 2005, Optics express.

[7]  D. Garner.  Flow cytometric sexing of mammalian sperm. , 2006, Theriogenology.

[8]  H. Kreuzer,et al.  Immersion digital in-line holographic microscopy. , 2006, Optics letters.

[9]  G. Coppola,et al.  A digital holographic microscope for complete characterization of microelectromechanical systems , 2004 .

[10]  E. S. E. Hafez,et al.  Reproduction in farm animals , 1974 .

[11]  Pietro Ferraro,et al.  Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging. , 2003, Applied optics.

[12]  Zeev Zalevsky,et al.  Superresolution digital holographic microscopy for three-dimensional samples. , 2008, Optics express.

[13]  Giancarlo Pedrini,et al.  Aberration compensation in digital holographic reconstruction of microscopic objects , 2001 .

[14]  W. Marsden.  I and J , 2012 .

[15]  D. Franken.  The clinical significance of sperm-zona pellucida binding. , 1998, Frontiers in bioscience : a journal and virtual library.

[16]  A Finizio,et al.  Correct-image reconstruction in the presence of severe anamorphism by means of digital holography. , 2001, Optics letters.

[17]  P. Ferraro,et al.  Quantitative phase-contrast microscopy by a lateral shear approach to digital holographic image reconstruction. , 2006, Optics letters.

[18]  Christian Depeursinge,et al.  Simultaneous cell morphometry and refractive index measurement with dual-wavelength Digital Holographic Microscopy , 2008 .

[19]  E. Cuche,et al.  Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. , 2005, Optics letters.

[20]  E. Cuche,et al.  Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. , 1999, Applied optics.

[21]  Etienne Cuche,et al.  Digital holographic microscopy: a new optical imaging technique to investigate cellular dynamics , 2006, SPIE Photonics Europe.

[22]  R. Stephenson.  A and V , 1962, The British journal of ophthalmology.

[23]  E. Cuche,et al.  Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy. , 2005, Optics express.

[24]  K. Badizadegan,et al.  Live cell refractometry using microfluidic devices. , 2006, Optics letters.

[25]  R. Kiss,et al.  Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration. , 2006, Journal of biomedical optics.

[26]  Bahram Javidi,et al.  Automatic identification of biological microorganisms using three-dimensional complex morphology. , 2006, Journal of biomedical optics.

[27]  H Ohzu,et al.  Hybrid holographic microscopy free of conjugate and zero-order images. , 1999, Applied optics.

[28]  F. Dubois,et al.  Improved three-dimensional imaging with a digital holography microscope with a source of partial spatial coherence. , 1999, Applied optics.

[29]  U. Schnars,et al.  Direct recording of holograms by a CCD target and numerical reconstruction. , 1994, Applied optics.

[30]  M. Allen,et al.  The natural subcellular surface structure of the bovine sperm cell. , 1995, Journal of structural biology.

[31]  Bahram Javidi,et al.  Three-dimensional imaging and recognition of microorganism using single-exposure on-line (SEOL) digital holography. , 2005, Optics express.