Study on the novel composite film composed of chitosan and poly(γ-benzyl L-glutamate)-co-poly(glutamic acid)

The novel composite film was prepared by chitosan and poly(γ-benzyl L-glutamate)-co-poly(glutamic acid),which were crosslinked by nontoxic crosslinking reagents of 1-ethyl-(dimethylaminopropyl) carbodiimide(EDC) and N-hydroxysuccinimide(NHS).The structure of the composite film was characterized by FTIR and XRD and the properties of water absorbency and in vitro biocompatibility were studied at the same time.The results indicated that the chitosan and PBLG-co-PGA were successfully crosslinked and the intrinsic crystal structures of the both materials were destroyed because of the intermolecular crosslinking and hydrogen-band action.The change of crystalline structures had an effect on the properties of the film.The results of culturing chondrocytes showed that there was the higher cell adherent ratio and the better cell configuration on the composite film than the pure chitosan film,which indicated that the composite film could become a promising material used in biomedical field.