Translational initiation in prokaryotes.

INTRODUCTION 365 BIOCHEMISTRY OF TRANSLATIONAL INITIATION 367 A CONCEPTUAL FRAMEWORK: RIBOSOME BINDING SITE STRENGTHS 373 THE NATURE OF RIBOSOME BINDING SITES 374 A First Approximation 374 Three Approaches to a Higher Order Approximation 380 Genetics and biochemistry 380 Statistics .......•••••••••.••..•.••.•...•.•........•....•••••••••••••••••• •.•••••••••• •.........•.• 382 DETERMINANTS: SEQUENCE AND/OR STRUCTURE ... 388 Pathways to Initiation 388 Unstructured Signals 389 Structured Initiation Regions 389 TRANSLATIONAL REGULATION 392 Unstructured RNAs 393 RNAs Whose Structures Seem Obvious 394 Complex Translational Regulation 396