Current and future prospects for the use of pulsed electric field in the meat industry

ABSTRACT Pulsed electric field (PEF) is a novel non-thermal technology that has recently attracted the attention of meat scientists and technologists due to its ability to modify membrane structure and enhance mass transfer. Several studies have confirmed the potential of pulsed electric field for improving meat tenderness in both pre-rigor and post-rigor muscles during aging. However, there is a high degree of variability between studies and the underlying mechanisms are not clearly understood. While some studies have suggested physical disruption as the main cause of PEF induced tenderness, enzymatic nature of the tenderization seems to be the most plausible mechanism. Several studies have suggested the potential of PEF to mediate the tenderization process due to its membrane altering properties causing early release of calcium ions and early activation of the calpain proteases. However, experimental research is yet to confirm this postulation. Recent studies have also reported increased post-mortem proteolysis in PEF treated muscles during aging. PEF has also been reported to accelerate curing, enhance drying and reduce the numbers of both pathogens and spoilage organisms in meat, although that demands intense processing conditions. While tenderization, meat safety and accelerated curing appears to be the areas where PEF could provide attractive options in meat processing, further research is required before the application of PEF becomes a commercial reality in the meat industry. It needs to deal with carcasses which vary biochemically and in composition (muscle, fat, and bones). This review critically evaluates the published reports on the topic with the aim of reaching a clear understanding of the possible applications of PEF in the meat sector in addition to providing some insight on critical issues that need to be addressed for the technology to be a practical option for the meat industry.

[1]  Da‐Wen Sun,et al.  Enhancement of Food Processes by Ultrasound: A Review , 2015, Critical reviews in food science and nutrition.

[2]  Stefan Toepfl,et al.  Pulsed Electric Field Processing of Orange Juice: A Review on Microbial, Enzymatic, Nutritional, and Sensory Quality and Stability. , 2013, Comprehensive reviews in food science and food safety.

[3]  M. Morzel,et al.  Chemical oxidation decreases proteolytic susceptibility of skeletal muscle myofibrillar proteins. , 2006, Meat science.

[4]  A. McAloon,et al.  Cost Analysis and Environmental Impact of Pulsed Electric Fields and High Pressure Processing in Comparison with Thermal Pasteurization , 2014, Food and Bioprocess Technology.

[5]  P. Franks,et al.  Physical Interventions to Manipulate Texture and Tenderness of Fresh Meat: A Review , 2014 .

[6]  D. Troy,et al.  Effects of electrical stimulation, chilling temperature and hot-boning on the tenderness of bovine muscles. , 2006, Meat science.

[7]  Eugène Vorobiev,et al.  Plasmolysis of sugarbeet : Pulsed electric fields and thermal treatment , 2007 .

[8]  V. Santé-Lhoutellier,et al.  Effect of oxidation on in vitro digestibility of skeletal muscle myofibrillar proteins. , 2007, Journal of agricultural and food chemistry.

[9]  A. Bekhit,et al.  Manipulation of Meat Quality: Electrical Stimulation and Pulsed Electric Field , 2017 .

[10]  W. Hamilton,et al.  Effects of high electric fields on microorganisms: II. Mechanism of action of the lethal effect , 1967 .

[11]  J. Sheridan,et al.  The ineffectiveness of organic acids, freezing and pulsed electric fields to control Escherichia coli O157:H7 in beef burgers , 2002, Letters in applied microbiology.

[12]  James C. Weaver,et al.  Theory of Electroporation , 1989 .

[13]  Alaa El-Din A. Bekhit,et al.  Effect of Pulsed Electric Field Treatment on Cold-Boned Muscles of Different Potential Tenderness , 2014, Food and Bioprocess Technology.

[14]  A. Bekhit,et al.  Effect of low and high pulsed electric field on the quality and nutritional minerals in cold boned beef M. longissimus et lumborum , 2017 .

[15]  D. Knorr,et al.  Investigating the potential of polyphenol oxidase as a temperature-time-indicator for pulsed electric field treatment , 2012 .

[16]  Y. Lan,et al.  Reduction of Escherichia coli O157:H7 on goat meat surface with pulsed dc square wave signal , 2006 .

[17]  Eduardo Puértolas,et al.  Electrotechnologies applied to valorization of by-products from food industry: Main findings, energy and economic cost of their industrialization , 2016 .

[18]  James G. Lyng,et al.  Shelf life and sensory attributes of a fruit smoothie-type beverage processed with moderate heat and pulsed electric fields , 2010 .

[19]  S. Toepfl,et al.  Review: Potential of High Hydrostatic Pressure and Pulsed Electric Fields for Energy Efficient and Environmentally Friendly Food Processing , 2006 .

[20]  R. Buckow,et al.  Opportunities and challenges in pulsed electric field processing of dairy products , 2014 .

[21]  O. Martín‐Belloso,et al.  Effects of High-Intensity Pulsed Electric Fields Processing Parameters on the Chlorophyll Content and Its Degradation Compounds in Broccoli Juice , 2014, Food and Bioprocess Technology.

[22]  Wei Zhao,et al.  Investigation of the protein-protein aggregation of egg white proteins under pulsed electric fields. , 2009, Journal of agricultural and food chemistry.

[23]  A. Borderías,et al.  Use of microbial transglutaminase and sodium alginate in the preparation of restructured fish models using cold gelation: Effect of frozen storage , 2010 .

[24]  E. Gayán,et al.  Inactivation of Salmonella Typhimurium and Staphylococcus aureus by pulsed electric fields in liquid whole egg , 2010 .

[25]  B. Wowk Electric and magnetic fields in cryopreservation. , 2012, Cryobiology.

[26]  L. Chernomordik,et al.  Reversible electrical breakdown of lipid bilayers: formation and evolution of pores. , 1988, Biochimica et biophysica acta.

[27]  Zhengxing Chen,et al.  Inactivation of soybean lipoxygenase in soymilk by pulsed electric fields. , 2008, Food chemistry.

[28]  E. Vorobiev,et al.  Pulsed electric field assisted aqueous extraction of colorants from red beet , 2011 .

[29]  J. Kerry,et al.  Impact of ingredient replacers on the physicochemical properties and sensory quality of reduced salt and fat black puddings. , 2016, Meat science.

[30]  M. C. Pina-Pérez,et al.  Effect of pulsed electric fields (PEF) combined with natural antimicrobial by-products against S. Typhimurium , 2016 .

[31]  P. Bremer,et al.  Reduction of bacterial counts and inactivation of enzymes in bovine whole milk using pulsed electric fields , 2014 .

[32]  A. Wiktor,et al.  Drying Kinetics of Apple Tissue Treated by Pulsed Electric Field , 2013 .

[33]  S. Töpfl,et al.  Pulsed Electric Fields (PEF) for Permeabilization of Cell Membranes in Food- and Bioprocessing – Applications, Process and Equipment Design and Cost Analysis. , 2006 .

[34]  K. Patel,et al.  Pulsed Electric Field Processing in Food Technology , 2015 .

[35]  M. Cano,et al.  Impact of food matrix and processing on the in vitro bioaccessibility of vitamin C, phenolic compounds, and hydrophilic antioxidant activity from fruit juice-based beverages , 2015 .

[36]  Francesco Noci,et al.  An assessment of the effect of pulsed electrical fields on tenderness and selected quality attributes of post rigour beef muscle. , 2013, Meat science.

[37]  E. S. Park,et al.  Origins of the Sensitivity of Molecular Vibrations to Electric Fields: Carbonyl and Nitrosyl Stretches in Model Compounds and Proteins , 2002 .

[38]  James C. Weaver,et al.  Electroporation of cells and tissues , 2000 .

[39]  J. Kerry,et al.  Consumer perception and the role of science in the meat industry. , 2010, Meat science.

[40]  E. Ortega-Rivas,et al.  Critical Issues Pertaining to Application of Pulsed Electric Fields in Microbial Control and Quality of Processed Fruit Juices , 2011 .

[41]  Eduardo Puértolas,et al.  Evolution of polyphenolic compounds in red wine from Cabernet Sauvignon grapes processed by pulsed electric fields during aging in bottle , 2010 .

[42]  Gustavo V. Barbosa-Cánovas,et al.  Inactivation of Microorganisms in a Semisolid Model Food Using High Voltage Pulsed Electric Fields , 1994 .

[43]  S Jeyamkondan,et al.  Pulsed electric field processing of foods: a review. , 1999, Journal of food protection.

[44]  James G. Lyng,et al.  The effect of pulsed electric field pre-treatments prior to deep-fat frying on quality aspects of potato fries , 2015 .

[45]  F. Barba,et al.  New Approaches for the Use of Non-conventional Cell Disruption Technologies to Extract Potential Food Additives and Nutraceuticals from Microalgae , 2015, Food Engineering Reviews.

[46]  F. Dunshea,et al.  Systematic review of emerging and innovative technologies for meat tenderisation. , 2017, Meat science.

[47]  E. Vorobiev,et al.  Temperature enhanced electroporation under the pulsed electric field treatment of food tissue , 2005 .

[48]  D. Knorr,et al.  High pressure, thermal and pulsed electric-field-induced structural changes in selected food allergens. , 2010, Molecular nutrition & food research.

[49]  J. Kerry,et al.  Salt reduction strategies in processed meat products – A review , 2017 .

[50]  F. Barba,et al.  Current and New Insights in the Sustainable and Green Recovery of Nutritionally Valuable Compounds from Stevia rebaudiana Bertoni. , 2015, Journal of agricultural and food chemistry.

[51]  M. V. van Boekel,et al.  Pulsed electric field processing of different fruit juices: impact of pH and temperature on inactivation of spoilage and pathogenic micro-organisms. , 2014, International journal of food microbiology.

[52]  Francesco Noci,et al.  An assessment of the impact of pulsed electric fields processing factors on oxidation, color, texture, and sensory attributes of turkey breast meat. , 2015, Poultry science.

[53]  A. Martı́neza,et al.  Effect of PEF and heat pasteurization on the physical–chemical characteristics of blended orange and carrot juice , 2005 .

[54]  A. Bekhit,et al.  Effect of Pulsed Electric Field Treatment on the Eating and Keeping Qualities of Cold-Boned Beef Loins: Impact of Initial pH and Fibre Orientation , 2015, Food and Bioprocess Technology.

[55]  Gustavo V. Barbosa-Cánovas,et al.  INACTIVATION OF ESCHERICHIA COLI SUSPENDED IN LIQUID EGG USING PULSED ELECTRIC FIELDS , 1997 .

[56]  E. Vorobiev,et al.  Acceleration of soluble matter extraction from chicory with pulsed electric fields , 2010 .

[57]  V. Santé-Lhoutellier,et al.  Effect of meat cooking on physicochemical state and in vitro digestibility of myofibrillar proteins. , 2008, Journal of agricultural and food chemistry.

[58]  Kevin Kantono,et al.  Effect of chilled and freezing pre-treatments prior to pulsed electric field processing on volatile profile and sensory attributes of cooked lamb meats , 2016 .

[59]  James G. Lyng,et al.  The use of pulsed electric fields for accelerating the salting of pork , 2014 .

[60]  Pedro Elez-Martínez,et al.  Pulsed Electric Field Processing , 2012 .

[61]  Charis M. Galanakis,et al.  The Effects of Conventional and Non-conventional Processing on Glucosinolates and Its Derived Forms, Isothiocyanates: Extraction, Degradation, and Applications , 2014, Food Engineering Reviews.

[62]  Dietrich Knorr,et al.  Evaluation of quality changes of blueberry juice during refrigerated storage after high-pressure and pulsed electric fields processing , 2012 .

[63]  Xiaosong Hu,et al.  Reduction of diazinon and dimethoate in apple juice by pulsed electric field treatment. , 2012, Journal of the science of food and agriculture.

[64]  M. S. Brewer,et al.  EFFECT OF HIGH‐INTENSITY PULSED ELECTRIC FIELDS ON SURVIVAL OF ESCHERICHIA COLI K‐12 SUSPENDED IN MEAT INJECTION SOLUTIONS , 2007 .

[65]  Gintautas Saulis,et al.  Electroporation of Cell Membranes: The Fundamental Effects of Pulsed Electric Fields in Food Processing , 2010 .

[66]  S. Toepfl,et al.  Applications of Pulsed Electric Fields Technology for the Food Industry , 2006 .

[67]  A. Bekhit,et al.  Effect of pulsed electric field treatment on hot-boned muscles of different potential tenderness. , 2015, Meat science.

[68]  Francisco J. Barba,et al.  Innovative Technologies for Food Preservation , 2018 .

[69]  S. Leong,et al.  Effect of pulsed electric fields on the structure and frying quality of “kumara” sweet potato tubers , 2017 .

[70]  D. Knorr,et al.  Pulsed electric fields and their impact on the diffusion characteristics of potato slices , 2011 .

[71]  A. Bekhit,et al.  Effect of low and high pulsed electric field processing on macro and micro minerals in beef and chicken , 2018 .

[72]  Henry Jaeger,et al.  Basics for Modeling of Pulsed Electric Field Processing of Foods , 2011 .

[73]  Harjinder Singh,et al.  Microstructure and protein digestibility of beef: The effect of cooking conditions as used in stews and curries , 2014 .

[74]  Judith Evans,et al.  The use of supercooling for fresh foods: A review , 2015 .

[75]  J. Raso,et al.  Improvements in the aqueous extraction of polyphenols from borage (Borago officinalis L.) leaves by pulsed electric fields: Pulsed electric fields (PEF) applications , 2015 .

[76]  Phil Bremer,et al.  Effect of freezing as pre-treatment prior to pulsed electric field processing on quality traits of beef muscles , 2015 .

[77]  Francesco Noci,et al.  Effect of pulsed electric field treatments at various stages during conditioning on quality attributes of beef longissimus thoracis et lumborum muscle. , 2015, Meat science.

[78]  S. Condón,et al.  Microbiological Aspects Related to the Feasibility of PEF Technology for Food Pasteurization , 2014, Critical reviews in food science and nutrition.

[79]  M. Gudmundsson,et al.  Effect of electric field pulses on microstructure of muscle foods and roes , 2001 .

[80]  M. Griffiths,et al.  Processing temperature, alcohol and carbonation levels and their impact on pulsed electric fields (PEF) mitigation of selected characteristic microorganisms in beer , 2011 .

[81]  D. J. Morgan,et al.  Combined effect of temperature and pulsed electric fields on apple juice peroxidase and polyphenoloxidase inactivation. , 2008, Food chemistry.

[82]  J. Raso,et al.  Effects of Pulsed Electric Field on Yield Extraction and Quality of Olive Oil , 2013, Food and Bioprocess Technology.

[83]  Phil Bremer,et al.  Effects of pH, temperature and pulsed electric fields on the turbidity and protein aggregation of ovomucin-depleted egg white. , 2017, Food research international.

[84]  Dietrich Knorr,et al.  4 – Overview of Pulsed Electric Field Processing for Food , 2005 .

[85]  S. Toepfl,et al.  Mass Transport Improvement by PEF - Applications in the Area of Extraction and Distillation , 2012 .

[86]  M. Ngadi,et al.  Synergistic effect of temperature and pulsed electric field on inactivation of Escherichia coli O157:H7 and Salmonella enteritidis in liquid egg yolk , 2007 .

[87]  G. Geesink,et al.  Contribution of postmortem muscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system. , 2006, Meat science.

[88]  Volker Heinz,et al.  Overview of Pulsed Electric Fields Processing for Food , 2005 .

[89]  Thomas Ohlsson,et al.  Simulation of the temperature increase in pulsed electric field (PEF) continuous flow treatment chambers , 2002 .

[90]  A. Bekhit,et al.  Effect of repeated pulsed electric field treatment on the quality of hot-boned beef loins and topsides. , 2016, Meat science.

[91]  G. Ferrari,et al.  Metal release from stainless steel electrodes of a PEF treatment chamber: Effects of electrical parameters and food composition , 2012 .

[92]  E. Stadtman,et al.  Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions. , 1993, Annual review of biochemistry.

[93]  M. Griffiths,et al.  Cross-protective effects of temperature, pH, and osmotic and starvation stresses in Escherichia coli O157:H7 subjected to pulsed electric fields in milk , 2011 .

[94]  R. Singh,et al.  A model stomach system to investigate disintegration kinetics of solid foods during gastric digestion. , 2008, Journal of food science.

[95]  S. Toepfl,et al.  Effect of High-Intensity Electric Field Pulses on Solid Foods , 2014 .

[96]  Flavien Pillet,et al.  Cell wall as a target for bacteria inactivation by pulsed electric fields , 2016, Scientific Reports.

[97]  Fumiyoshi Tochikubo,et al.  Efficient sterilization of bacteria by pulse electric field in micro-gap , 2008 .

[98]  I. Oey,et al.  Impact of pulsed electric fields and post‐mortem vacuum ageing on beef longissimus thoracis muscles , 2014 .

[99]  Dietrich Knorr,et al.  Impact of temperature on lethality and energy efficiency of apple juice pasteurization by pulsed electric fields treatment , 2003 .

[100]  G. Barbosa‐Cánovas,et al.  Effect of processing parameters on inactivation of Bacillus cereus spores in milk using pulsed electric fields , 2012 .

[101]  Wei Zhao,et al.  Recent advances in the action of pulsed electric fields on enzymes and food component proteins , 2012 .

[102]  Gauri S. Mittal,et al.  Pasteurization of Milk Using Pulsed Electrical Field and Antimicrobials , 2002 .

[103]  M. Hanna,et al.  Enhanced anthocyanin extraction from red cabbage using pulsed electric field processing. , 2010, Journal of food science.

[104]  A. Gaspar,et al.  Action of microbial transglutaminase (MTGase) in the modification of food proteins: a review. , 2015, Food chemistry.

[105]  Mahendra Pal,et al.  Pulsed Electric Field Processing: An Emerging Technology for Food Preservation , 2017 .

[106]  Soojin Jun,et al.  Emerging pulsed electric field (PEF) and static magnetic field (SMF) combination technology for food freezing , 2015 .

[107]  I. Arvanitoyannis Novel Food Processing Technologies , 2006 .

[108]  J. Weaver,et al.  Theory of electroporation: A review , 1996 .

[109]  R P Joshi,et al.  Improved energy model for membrane electroporation in biological cells subjected to electrical pulses. , 2002, Physical review. E, Statistical, nonlinear, and soft matter physics.

[110]  A. Bekhit,et al.  Effect of Repeated Pulsed Electric Field Treatment on the Quality of Cold-Boned Beef Loins and Topsides , 2015, Food and Bioprocess Technology.

[111]  A. Bekhit,et al.  Quality and Nutritional Minerals in Chicken Breast Muscle Treated with Low and High Pulsed Electric Fields , 2017, Food and Bioprocess Technology.

[112]  P. Butz,et al.  Extraction of anthocyanins from grape by-products assisted by ultrasonics, high hydrostatic pressure or pulsed electric fields: A comparison , 2008 .

[113]  T J Mason,et al.  Power ultrasound in meat processing. , 2015, Meat science.

[114]  Ulrich Zimmermann,et al.  Effects of external electrical fields on cell membranes , 1976 .

[115]  Dietrich Knorr,et al.  High intensity pulsed electric fields applied for food preservation , 2007 .

[116]  James C. Weaver,et al.  Electroporation: a unified, quantitative theory of reversible electrical breakdown and mechanical rupture in artificial planar bilayer membranes☆ , 1991 .

[117]  F. Barba,et al.  “Ice” juice from apples obtained by pressing at subzero temperatures of apples pretreated by pulsed electric fields , 2016 .

[118]  J. Teissié,et al.  Evidence for conduction of protons along the interface between water and a polar lipid monolayer. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[119]  S. Töpfl,et al.  Application of pulsed electric fields to improve mass transfer in dry cured meat products , 2007 .

[120]  Wei Zhao,et al.  Lethal and sublethal injury and kinetics of Escherichia coli, Listeria monocytogenes and Staphylococcus aureus in milk by pulsed electric fields , 2013 .

[121]  S. Jun,et al.  Effects of pulsed electric field (PEF) and oscillating magnetic field (OMF) combination technology on the extension of supercooling for chicken breasts , 2017 .

[122]  I. Díaz,et al.  Influence of high pressure application on the nutritional, sensory and microbiological characteristics of sliced skin vacuum packed dry-cured ham. Effects along the storage period , 2011 .

[123]  D. J. Morgan,et al.  Effectiveness of combined Pulsed Electric Field (PEF) and Manothermosonication (MTS) for the control of Listeria innocua in a smoothie type beverage , 2012 .

[124]  W. Lewandowski,et al.  Influence of pulsed electric field on the survival of Yersinia enterocolitica in minced beef meat : assessment of microbiological activity of selected cell lines of bacteria under influence of physical-chemical factors , 2012 .

[125]  J. Lyng,et al.  Impact of pulsed light on colour, carotenoid, polyacetylene and sugar content of carrot slices , 2017 .

[126]  Eugène Vorobiev,et al.  Effects of Pulsed Electric Fields on Cabernet Sauvignon Grape Berries and on the Characteristics of Wines , 2014, Food and Bioprocess Technology.

[127]  Volker Heinz,et al.  Pulsed electric fields technology for the food industry : fundamentals and applications , 2006 .

[128]  I. Oey,et al.  Thermal properties of milk fat, xanthine oxidase, caseins and whey proteins in pulsed electric field-treated bovine whole milk. , 2016, Food chemistry.

[129]  E. Gayán,et al.  Design of a combined process for the inactivation of Salmonella Enteritidis in liquid whole egg at 55°C. , 2011, International journal of food microbiology.

[130]  A. Wiktor,et al.  Pulsed Electric Field Pretreatment for Osmotic Dehydration of Apple Tissue: Experimental and Mathematical Modeling Studies , 2014 .

[131]  A. Bekhit,et al.  Effect of pulsed electric field on the proteolysis of cold boned beef M. Longissimus lumborum and M. Semimembranosus. , 2015, Meat science.

[132]  James G. Lyng,et al.  Efficacy of pulsed electric fields for the inactivation of indicator microorganisms and foodborne pathogens in liquids and raw chicken , 2012 .

[133]  Wei Zhao,et al.  Effects of pulsed electric fields processing on stability of egg white proteins , 2014 .

[134]  Zhengxing Chen,et al.  Effects of pulsed electric fields on physicochemical properties of soybean protein isolates , 2007 .

[135]  Filipa V. M. Silva,et al.  Pulsed Electric Field continuous pasteurization of different types of beers , 2015 .

[136]  V. Santé-Lhoutellier,et al.  Digestion study of proteins from cooked meat using an enzymatic microreactor. , 2009, Meat science.

[137]  A. Bekhit,et al.  Exogenous Proteases for Meat Tenderization , 2014, Critical reviews in food science and nutrition.

[138]  R. Mendes,et al.  The influence of fish age, salt level, and MTGase addition on the quality of gels prepared from unwashed mince of Farmed Meagre (Argyrosomus regius) , 2014, Food science and technology international = Ciencia y tecnologia de los alimentos internacional.