Breath-Analysis Using Mid-Infrared Tunable Laser Spectroscopy

Breath analysis is becoming an attractive technique for the noninvasive monitoring of various molecular constituents in exhaled breath. Nitric oxide (NO) is a widely studied biomarker in breath and is known to indicate the lower airway inflammation associated with asthma. A mid-IR laser-based spectroscopic sensor and associated electronics were designed to measure exhaled NO simultaneously with CO2. To evaluate the sensor performance, a large observational clinical field study consisting of more than 2200 volunteers was performed over a period of two years. The generated distributions of the measured exhaled NO and CO2 levels were found to be log-normal and Gaussian, respectively. In addition, preliminary results of measuring exhaled nitrous oxide (N2O) are given as well as suggestions for future applications in infectious disease.

[1]  C. Krehbiel,et al.  Measurement of exhaled nitric oxide in beef cattle using tunable diode laser absorption spectroscopy. , 2007, Applied optics.

[2]  Frank K. Tittel,et al.  Sub-ppbv nitric oxide concentration measurements using cw thermoelectrically cooled quantum cascade laser-based integrated cavity output spectroscopy , 2006 .

[3]  A. Cho,et al.  Spectroscopic detection of biological NO with a quantum cascade laser , 2001, Applied physics. B, Lasers and optics.

[4]  David Smith,et al.  Quantification of acetaldehyde released by lung cancer cells in vitro using selected ion flow tube mass spectrometry. , 2003, Rapid communications in mass spectrometry : RCM.

[5]  Miles J Padgett,et al.  Development of high-resolution real-time sub-ppb ethane spectroscopy and some pilot studies in life science. , 2005, Applied optics.

[6]  L R Narasimhan,et al.  Correlation of breath ammonia with blood urea nitrogen and creatinine during hemodialysis , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[7]  Khosrow Namjou,et al.  Nitric oxide breath testing by tunable-diode laser absorption spectroscopy: application in monitoring respiratory inflammation. , 2002, Applied optics.

[8]  A. Clifford,et al.  Quantitative analysis by gas chromatography of volatile carbonyl compounds in expired air from mice and human. , 1997, Journal of chromatography. B, Biomedical sciences and applications.

[9]  K. Namjou,et al.  Determination of exhaled nitric oxide distributions in a diverse sample population using tunable diode laser absorption spectroscopy , 2006 .