Identification of the tetracycline resistance promoter and repressor in transposon Tn10

The structural and regulatory functions encoding tetracycline resistance in transposon Tn10 lie within a 2,700-base pair region. Using recombinant plasmids with different deoxyribonucleic acid sequences adjacent to a HincII site in this region, we located the promoter controlling the expression of tetracycline resistance. These various sequences conferred altered levels of tetracycline resistance. Plasmids containing deletions of a 695-base pair HincII fragment were constitutive and showed the loss of a 23,000-dalton tetracycline-inducible polypeptide, thus identifying the repressor and the location of its gene.

[1]  S. Levy,et al.  Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[2]  D. Court,et al.  Promoter for the establishment of repressor synthesis in bacteriophage lambda. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[3]  G. Bogosian,et al.  DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor. , 1980, Nucleic acids research.

[4]  R. Brent,et al.  The lexA gene product represses its own promoter. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[5]  W. Reznikoff,et al.  The inverted repeats of Tn5 are functionally different , 1980, Cell.

[6]  F. Heffron,et al.  DNA sequence analysis of the transposon Tn3: Three genes and three sites involved in transposition of Tn3 , 1979, Cell.

[7]  S. Cohen,et al.  Identification and characterization of a self-regulated repressor of translocation of the Tn3 element. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[8]  F. Bolivar,et al.  Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313. , 1979, Nucleic acids research.

[9]  R. Wells,et al.  Cloning and localization of the in vitro functional origin of replication of bacteriophage T7 DNA. , 1979, The Journal of biological chemistry.

[10]  W. Reznikoff,et al.  Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10 , 1979, Journal of bacteriology.

[11]  M. Calos,et al.  DNA sequence for a low-level promoter of the lac repressor gene and an ‘up’ promoter mutation , 1978, Nature.

[12]  P. Seeburg,et al.  Interaction of RNA polymerase with promoters from bacteriophage fd. , 1977, European journal of biochemistry.

[13]  F. Sanger,et al.  Nucleotide sequence of bacteriophage φX174 DNA , 1977, Nature.

[14]  W. Gilbert,et al.  A new method for sequencing DNA. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[15]  H. Yang,et al.  Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[16]  K. Sugimoto,et al.  Sequence of promoter for coat protein gene of bacteriophage fd , 1976, Nature.

[17]  M. Ptashne,et al.  Novel properties of a restriction endonuclease isolated from Haemophilus parahaemolyticus. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[18]  D. Pribnow,et al.  Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites. , 1975, Journal of molecular biology.

[19]  M. Ptashne,et al.  Lambda repressor turns off transcription of its own gene. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[20]  D. Botstein,et al.  Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. , 1975, Journal of molecular biology.

[21]  F. Blattner,et al.  Hybridization of labeled RNA to DNA in agarose gels. , 1975, Nucleic acids research.

[22]  J. M. Robertson,et al.  The characteristics of eleven mutants of R-factor R57 constitutive for tetracycline resistance, selected and tested in Escherichia coli K12. , 1975, Genetical research.

[23]  E. S. Anderson,et al.  A simple method for the preparation of large quantities of pure plasmid DNA. , 1975, Biochimica et biophysica acta.

[24]  D. Pribnow.  Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[25]  T. Maniatis,et al.  Nucleotide sequence of the rightward operator of phage lambda. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[26]  C. Gray,et al.  Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[27]  W. Reznikoff,et al.  Genetic regulation: the Lac control region. , 1975, Science.

[28]  S. Baumberg,et al.  Control regions within the argECBH gene cluster of Escherichia coli K12 , 1972, Molecular and General Genetics MGG.

[29]  A. Guha.  Divergent orientation of transcription from the biotin locus of Escherichia coli. , 1971, Journal of molecular biology.

[30]  M Mandel,et al.  Calcium-dependent bacteriophage DNA infection. , 1970, Journal of molecular biology.

[31]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[32]  K. Arima,et al.  Specificity and Mechanism of Tetracycline Resistance in a Multiple Drug Resistant Strain of Escherichia coli , 1966, Journal of bacteriology.

[33]  Elmer S. West.  From the U. S. A. , 1965 .

[34]  M Ptashne,et al.  Sequence of a repressor-binding site in the DNA of bacteriophage lamda. , 1974, Nature.

[35]  Jeffrey H. Miller.  Experiments in molecular genetics , 1972 .